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Algicidal activity of polyunsaturated fatty acids derived from Ulva fasciata and U. pertusa (Ulvaceae, Chlorophyta) on phytoplankton

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Abstract

Isolation of algicidal compounds from Ulva fasciata revealed that the algicidal substances were the polyunsaturated fatty acids (PUFAs) as hexadeca-4,7,10,13-tetraenoic acid (HDTA) C16:4 n-3, octadeca-6,9,12,15-tetraenoic acid (ODTA) C18:4 n-3, α-linolenic acid (ALA) C18:3 n-3 and linoleic acid (LA) C18:2 n-6. The fatty acid composition of four species of Ulvaceae (U. fasciata, U. pertusa, U. arasakii and U. conglobota) was analyzed by capillary gas chromatography to investigate the relationship with the algicidal activity. The results indicate that highly algicidal species, U. fasciata and U. pertusa, showed higher contents of C16:4 n-3, C18:3 n-3, and C18:4 n-3. Concentrations of these PUFAs released from the seaweed in the culture medium were also analyzed. These PUFAs were found to be significantly active against Chattonella antiqua, C. marina, Fibrocapsa japonica, Heterosigma akashiwo, Karenia mikimotoi, moderately effective against Heterocapsa circularisquama, Prorocentrum minimum, P. sigmoides, Scrippsiella trochoidea, whereas low effective against Alexandrium catenella and Cochlodinium polykrikoides. It is suggested that the PUFAs are useful mitigation agents to remove several harmful effects without causing detrimental effects on surrounding marine living organisms.

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Acknowledgements

We thank to the Ministry of Education, Culture, Sports, Science and Technology of Japan having given a part of these research funds to study. A part of this study was also supported financially by the Nagasaki Prefecture Collaboration of Regional Entities for the Advancement of Technological Excellence, Japan Science and Technology Agency (JST). We also thank to member of research group of Kashima-shi Fishery Cooperation, Saga Prefecture which had cooperate to study it.

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Authors and Affiliations

  1. Graduate School of Science and Technology, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, 852-8521, Japan

    Mochammad Amin Alamsjah, Shotaro Hirao, Fumito Ishibashi & Yuji Fujita

  2. Faculty of Fisheries, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, 852-8521, Japan

    Tatsuya Oda

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  1. Mochammad Amin Alamsjah
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  2. Shotaro Hirao
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  3. Fumito Ishibashi
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  4. Tatsuya Oda
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  5. Yuji Fujita
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Correspondence to Fumito Ishibashi.

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Alamsjah, M.A., Hirao, S., Ishibashi, F. et al. Algicidal activity of polyunsaturated fatty acids derived from Ulva fasciata and U. pertusa (Ulvaceae, Chlorophyta) on phytoplankton. J Appl Phycol 20, 713–720 (2008). https://doi.org/10.1007/s10811-007-9257-5

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  • DOI: https://doi.org/10.1007/s10811-007-9257-5

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