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Melatonin can Ameliorate Radiation-Induced Oxidative Stress and Inflammation-Related Deterioration of Bone Quality in Rat Femur

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ABSTRACT

The aim of the present study was to evaluate the radioprotective effects of melatonin on the biomechanical properties of bone in comparison to amifostine (WR-2721). Forty Sprague Dawley rats were divided equally into 5 groups namely; control (C), irradiation (R; single dose of 50 Gy), irradiation + WR-2721 (R + WR-2721; irradiation + 200 mg/kg WR-2721) radiation + melatonin 25 mg/kg (R + M25; irradiation + 25 mg/kg melatonin), and radiation + melatonin 50 mg/kg (R + M50; irradiation + 50 mg/kg melatonin). In order to measure extrinsic (organ-level mechanical properties of bone; the ultimate strength, deformation, stiffness, energy absorption capacity) and intrinsic (tissue-level mechanical properties of bone; ultimate stress, ultimate strain, elastic modulus, toughness) features of the bone, a three-point bending (TPB) test was performed for biomechanical evaluation. In addition, a bone mineral density (BMD) test was carried out. The BMD and extrinsic properties of the diaphyseal femur were found to be significantly higher in the R + M25 group than in group R (p < 0.05). A significant increase was observed in R + M50 (p < 0.05) in comparison to group R in the cross-sectional area of the femoral shaft and elastic modulus parameter. The protective effect of melatonin was similar to that of WR-2721. Thus, biomechanical quality of irradiated bone can be ameliorated by free radical scavenger melatonin.

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Author information

Authors and Affiliations

  1. Department of Biophysics, Faculty of Medicine, Gaziantep University, Gaziantep, TR-27310, Turkey

    Zelal Ünlü Çakir & Can Demirel

  2. Department of Radiation Oncology, Kolan International Hospital, Istanbul, TR-34000, Turkey

    Sevil Cagiran Kilciksiz

  3. Department of Biophysics, Faculty of Medicine, Gaziosmanpaşa University, Tokat, TR-60000, Turkey

    Serkan Gürgül

  4. Department of Radiation Oncology, Faculty of Medicine, Dicle University, Diyarbakır, TR-33169, Turkey

    S. Burhanedtin Zincircioğlu

  5. Department of Biophysics, Faculty of Medicine, Mersin University, Mersin, TR-33169, Turkey

    Nurten Erdal

Authors
  1. Zelal Ünlü Çakir
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  2. Can Demirel
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  3. Sevil Cagiran Kilciksiz
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  4. Serkan Gürgül
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Correspondence to Can Demirel.

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All experiments and protocols described in the present study were performed in accordance with the guidelines of the European Convention for the Protection of Vertebrate Animals used for Experimental and other Scientific Purposes and also approved by the Medical Faculty Experimentation Ethics Committee of Gaziantep University.

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The authors declare that they have no conflict of interest.

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Çakir, Z.Ü., Demirel, C., Kilciksiz, S.C. et al. Melatonin can Ameliorate Radiation-Induced Oxidative Stress and Inflammation-Related Deterioration of Bone Quality in Rat Femur. Inflammation 39, 1134–1140 (2016). https://doi.org/10.1007/s10753-016-0347-x

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  • DOI: https://doi.org/10.1007/s10753-016-0347-x

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