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Effects of different dietary phospholipid levels on growth performance, fatty acid composition, PPAR gene expressions and antioxidant responses of blunt snout bream Megalobrama amblycephala fingerlings

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Abstract

A 60-day feeding trial was conducted to evaluate the effects of different levels of dietary phospholipid (PL) from soybean lecithin on growth performance, liver fatty acid composition, peroxisome proliferator-activated receptor (PPAR) gene expression levels and antioxidant responses of blunt snout bream fingerlings. Fish (average initial weight 0.35 ± 0.01 g) were fed five experimental diets containing the following inclusion levels of PL: 0, 2, 4, 6 and 8 %. Results showed that final body weight, weight gain and specific growth rate increased significantly (P < 0.05) as dietary PL level increased from 0 to 6 %, meanwhile the survival was not affected by dietary PL supplementation. Increasing dietary PL level significantly (P < 0.05) increased in 20:4n-6 content in neutral lipid of liver, indicating fish had the capacity to convert C18 to C20 and C22 by elongation and desaturation. The expression levels of PPAR-α and PPAR-γ and the activities of catalase, superoxide dismutase and glutathione peroxidase in liver were significantly (P < 0.05) increased, and liver thiobarbituric acid reactive substances value was decreased with dietary PL supplementation up to 6 % compared with the control. Therefore, it was concluded that supplementation of 6 % (18.8 g kg−1, polar lipid of diet) PL could improve growth performance of blunt snout bream fingerlings.

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Acknowledgments

This study was supported by the Fundamental Research Funds for the Central Universities (Project 2013PY074).

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Authors and Affiliations

  1. College of Fisheries, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, China

    Yang Li, Jian Gao & Songqian Huang

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  1. Yang Li
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  2. Jian Gao
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Correspondence to Jian Gao.

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Li, Y., Gao, J. & Huang, S. Effects of different dietary phospholipid levels on growth performance, fatty acid composition, PPAR gene expressions and antioxidant responses of blunt snout bream Megalobrama amblycephala fingerlings. Fish Physiol Biochem 41, 423–436 (2015). https://doi.org/10.1007/s10695-014-9994-8

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