Skip to main content
SpringerLink
Log in

Long-term culture of a cell population from Siberian sturgeon (Acipenser baerii) head kidney

  • Published:
Fish Physiology and Biochemistry Aims and scope Submit manuscript

Abstract

In vitro cultures of native fish cell lines are of great importance, both for basic research and applied science. In particular, there is strong demand for long-term growable cell lines from breeding fish, like sturgeon. Here, we describe the culture of cells from Siberian sturgeon (Acipenser baerii) head kidney. The cells have so far been cultured over a period of 12 months (24 passages). Cytochemical and immunocytochemical examination suggests that, in vitro, the cells exhibit markers that are indicative for different cell types. In particular, fat storing cells (adipocytes) were observed, and the expression of cytokeratins and glial fibrilar acidic protein (GFAP) can be concluded on the basis of immuncytochemical analysis. The observation of different morphologies additionally underlines the heterogeneity of the cell population and matches the typical behaviour of in vitro cultures of stem/progenitor cells. Different applications can be imagined.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Braun-Nesje R, Kapplan G, Seljelid R (1982) Rainbow trout macrophages in vitro: Morphology and phagocytic activity. Dev Comp Immunol 6:281–291

    Article  PubMed  CAS  Google Scholar 

  • Catton WT (1951) Blood cell formation in certain teleost fishes. Blood 6: 39–60

    PubMed  CAS  Google Scholar 

  • Dishon A, Perelberg A, Bisharan-Shieban J, Ilouze M, Davidovich M, Werker S, Kotler M (2005) Detection of carp interstitial nephritis and gill necrosis virus in fish droppings. Appl Environ Microbiol 71:7285–7291

    Article  PubMed  CAS  Google Scholar 

  • Fänge R (1986) Lymphoid organs in sturgeons (Acipenseridae). Vet Immunol Immunopathol 12:153–161

    Article  PubMed  Google Scholar 

  • Friedenstein AJ (1991) Osteogenic stem cells in the bone marrow. Bone Miner 7:243–272

    Google Scholar 

  • Holen E, Hamre K (2004) Towards obtaining long term embryonic stem cell like cultures from a marine flatfish, Scophtalmus maximus. Fish Physiol Biochem 29:245–252

    Article  Google Scholar 

  • Jiang Y, Jahagirdar BN, Reinhardt RL, Schwartz RE, Keene CD, Ortiz-Gonzalez XR, Reyes M, Lenvik T, Lund T, Blackstad M, Du J, Aldrich S, Lisberg A, Low WC, Largaespada DA, Verfaillie CM (2007) Pluripotency of mesenchymal stem cells derived from adult marrow. Nature 447(7146):880–881

    Article  Google Scholar 

  • Joerink M, Ribeiro CMS, Stet RJM, Hermsen T, Savelkoul HFJ, Wiegertjes GF (2006) Head kidney-derived macrophages of common carp (Cyprinus carpio L.) show plasticity and functional polarization upon differential stimulation. J Immunol 177(1):61

    PubMed  CAS  Google Scholar 

  • Kruse C, Grunweller A, Notbohm H, Kugler S, Purschke WG, Muller PK (1996) Evidence for a novel cytoplasmatic tRNA-protein complex containing the KH-multidomain protein vigilin. Biochem J 320:247–252

    PubMed  CAS  Google Scholar 

  • Kruse C, Birth M, Rohwedel J, Assmuth K, Goepel A, Wedel T (2004) Pluripotency of adult stem cells derived from human and rat pancreas. Appl Phys A 79:1617–1624

    Article  CAS  Google Scholar 

  • Lemischka IR, Raulet DH, Mulligan RC (1986) Developmental potential and dynamic behaviour of haematopoietic stem cells. Cell 45(6):917–927

    Article  PubMed  CAS  Google Scholar 

  • Raymond PA, Barthel LK, Bernardos RL, Perkowski JJ (2006) Molecular characterization of retinal stem cells and their niches in adult zebrafish. BMC Dev Biol 6:36

    Article  PubMed  Google Scholar 

  • Serafini M, Verfaillie CM (2006) Pluripotency in adult stem cells: state of the art. Semin Reprod Med 24(5):379–388, Review

    Article  PubMed  CAS  Google Scholar 

  • Siegl E, Albrecht S, Lüdtke B (1993) Long-term liquid culture of haematopoietic precursor cells from the head kidney and spleen of the rainbow trout (oncorhynchus mykiss). Comp Haematol Int 3:168–173

    Article  Google Scholar 

  • Zapata A, Amemiya CT (2000) Phylogeny of lower vertebrates and their immunological structures. Curr Top Microbiol Immunol 248:67–107

    PubMed  CAS  Google Scholar 

  • Zapata AG, Chiba A, Varos A (1997) Cells and tissues of the immune system of fish. In: Iwama G, Nakanishi T (eds) The fish immune system organism, pathogen and environment. Academic Press, San Diego, pp 1–61

    Chapter  Google Scholar 

  • Zuasti A, Ferrer C (1989) Haematopoiesis in the head kidney of Sparus auratus. Arch Histol Cytol 52:249–255

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by grants of WTSH (Wirtschaftsförderung und Technologietransfer Schleswig-Holstein). The authors would like to thank Dr. Daniel Rapoport and Mrs. Jennifer Kajahn for the revision of the manuscript.

Author information

Authors and Affiliations

  1. Fraunhofer Institute for Marine Biotechnology, Fraunhofer Society, Maria-Goeppert-Str. 1, 23562, Luebeck, Germany

    P. Ciba, S. Schicktanz, E. Klink & C. Kruse

  2. Research Institute for Agriculture and Fishery, Mecklenburg-Vorpommern, Südstr. 8, 18375, Born/Darß, Germany

    E. Anders

  3. Institute for Biosciences, The University of Rostock, Wismarsche Str. 8, 18055, Rostock, Germany

    E. Siegl & A. Stielow

Authors
  1. P. Ciba
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Schicktanz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. Anders
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. Siegl
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. Stielow
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. E. Klink
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. C. Kruse
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to C. Kruse.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ciba, P., Schicktanz, S., Anders, E. et al. Long-term culture of a cell population from Siberian sturgeon (Acipenser baerii) head kidney. Fish Physiol Biochem 34, 367–372 (2008). https://doi.org/10.1007/s10695-007-9196-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10695-007-9196-8

Keywords

Search

Navigation