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Genetic analysis and identification of DNA markers linked to a novel Phytophthora sojae resistance gene in the Japanese soybean cultivar Waseshiroge

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Abstract

The Glycine max (L.) Merr. cultivar Waseshiroge is highly resistant to several races of Phytophthora sojae in Japan. In order to determine which Rps gene might be present in Waseshiroge, 15 differential cultivars were challenged with 12 P. sojae isolates. None had a reaction pattern identical to that of Waseshiroge, indicating that Waseshiroge may contain a novel Rps gene. In order to characterize the inheritance of Waseshiroge resistance to P. sojae isolates, 98 F2 progeny and 94 F7:8 lines were produced from crosses between the susceptible cultivar Tanbakuro and Waseshiroge. Chi-square tests indicated that segregation fit a 3:1 ratio for resistance and susceptibility in two F2 sub-populations of 42 and 56 seedlings. This and a 46.27:1.46:46.27 (or 63:2:63) ratio for resistance: segregation: susceptibility among the 94 F7:8 lines indicated that resistance was controlled by a single dominant gene. DNA analyses were carried out on Tanbakuro, Waseshiroge and the 94 F7:8 lines, and a linkage map was constructed with 17 SSR markers and nine new primer pairs that amplify marker loci linked to Rps1 on soybean chromosome 3 (linkage group N). The closest markers, Satt009 and T000304487l, map to locations 0.9 and 1.6 cM on each side of the estimated position of the Rps gene, respectively. The results showed that the Rps gene in Waseshiroge is either allelic to Rps1, or resides at a tightly linked locus in a gene cluster. A three-way-contingency table analysis indicated that marker-assisted selection with the two flanking markers could be used in the development of new resistant cultivars.

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Acknowledgments

The authors thank Professor Dr. Anne E. Dorrance of The Ohio State University for valuable discussions and for providing P. sojae isolates from Ohio; Eiji Hinomoto for collecting diseased plants; Tomoko Okudaira, Kozue Akamatsu, Toshiharu Ohnishi, Sanae Shikata, Natsuko Ichieda, Atsuko Mineyama, Masanobu Kawai, and Seiko Mori for supporting this study; and Akiko Tazawa and Yoko Yamashita (Hokkaido Prefectural Plant Genetic Resource Center), as well as Randall L. Nelson (USDA-ARS) for providing seeds of the soybean differential cultivars. This work was supported by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Genomics for Agricultural Innovation, DD-3113), Development of mitigation and adaptation techniques to global warming in the sectors of agriculture, forestry, and fisheries (1005), and by a grant from the Hyogo Prefectural Government (Overseas training programs for young staff, 2007).

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Authors and Affiliations

  1. Hyogo Agricultural Institute for Agriculture, Forestry and Fisheries, 1533 Minamino-oka, Kasai, Hyogo, 679-0198, Japan

    T. Sugimoto, S. Yoshida, K. Watanabe, M. Aino & K. Tatsuda

  2. National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8602, Japan

    A. Kaga & M. Ishimoto

  3. NARO Institute of Crop Science, National Agriculture and Food Research Organization, Kannondai 2-1-18, Tsukuba, Ibaraki, 305-8518, Japan

    M. Hajika

  4. National Agricultural Research Center for Tohoku Region (NARCT), 4 Akahira, Shimo-kuriyagawa, Morioka, Iwate, 020-0198, Japan

    R. Yamamoto

  5. Tokyo University of Agriculture, Department of Applied Biology and Chemistry, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo, 156-8502, Japan

    K. Watanabe

  6. Division of Applied Life Sciences, Laboratory of Plant Nutrition, Graduate School of Agriculture, Kyoto University, Oiwake, Kitashirakawa, Sakyo-ku, Kyoto, 606-8502, Japan

    T. Matoh

  7. USDA-ARS, Soybean/Maize Germplasm, Pathology, and Genetics Research Unit, 232 National Soybean Research Center, 1101 W. Peabody Dr, Urbana, IL, 61801, USA

    D. R. Walker

  8. Kearneysville Tree Fruit Research and Education Center, West Virginia University, P.O. Box 609, Kearneysville, WV, 25430, USA

    A. R. Biggs

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  1. T. Sugimoto
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  2. S. Yoshida
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  7. K. Tatsuda
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  8. R. Yamamoto
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  9. T. Matoh
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  10. D. R. Walker
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Correspondence to T. Sugimoto.

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Sugimoto, T., Yoshida, S., Kaga, A. et al. Genetic analysis and identification of DNA markers linked to a novel Phytophthora sojae resistance gene in the Japanese soybean cultivar Waseshiroge. Euphytica 182, 133–145 (2011). https://doi.org/10.1007/s10681-011-0525-8

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