Abstract
Herbivorous mammal dung supports a large variety of fimicolous fungi and the number of new genera and species is constantly increasing. Dung is a complex ecosystem and to win the struggle for life, these fungi produce a plethora of bioactive secondary metabolites to compete with other organisms. Fimicolous fungi and their bioactive metabolites are mostly evaluated for a possible use in medicine. Very little information is available about their possible exploitation in agriculture against plant pathogens. Three fimicolous isolates of Cleistothelebolus nipigonensis, Neogymnomyces virgineus and Rodentomyces reticulatus, collected from dung of different herbivorous were investigated. Only the organic extracts of Solid State Fermentation (SSF) cultures of C. nipigonensis and N. virgineus grown on rye flour showed strong antifungal activity against fungal pathogens of some important crops. The purification of the organic extracts allowed us to obtain different fractions and the isolation of some pure metabolites and their preliminary chemical and biological characterization are reported in this manuscript.
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Bartlett, D. W., Clough, J. M., Godwin, J. R., Hall, A. A., Hamer, M., & Parr-Dobrzanski, B. (2002). The strobilurin fungicides. Pest Management Science, 58, 649–662.
Berger, S., & Braun, S. (2004). 200 and more basic NMR experiments: A practical course (1st ed.). Weinheim: Wiley-VCH.
Bills, G. F., Gloer, J. B., & An, Z. (2013). Coprophilous fungi: antibiotic discovery and functions in an underexplored arena of microbial defensive mutualism. Current Opinion in Microbiology, 16, 1–17.
Breitmaier, E., & Voelter, W. (1987). Carbon-13 NMR spectroscopy (pp. 183–280). Weinheim: VCH.
Burges, A. (1958). Microorganisms in the soil. Hutchinson University London.
Domınguez, J. M., Martın, J. J. (2005). Sordarins: inhibitors of fungal elongation factor-2. In A Z. Marcel Dekker (Ed.) Handbook of Industrial Mycology. (pp. 335–353).
Doveri, F. (2004). Fungi fimicoli italici. AMB Fondazione Centro Studi Micologici.
Doveri, F., Pecchia, S., Sarrocco, S., Minnocci, F., & Vannacci, G. (2010). Rodentomyces, a new hypocrealean genus from Italy. Fungal Diversity, 42(1), 57–69.
Doveri, F., Pecchia, S., Vergara, M., Sarrocco, S., & Vannacci, G. (2012). A comparative study and relationship with Onygenales of Neogymnomyces virgineus, a new keratinolytic species from dung. Fungal Diversity, 52(1), 13–34.
Doveri, F., Sarrocco, S., & Vannacci, G. (2013). Studies on three rare coprophilous Plectomycetes from Italy. Mycotaxon, 124, 279–300.
Garret, S. D. (1963). Soil fungi and soil fertility. Oxford: Pergamon Press.
Gloer, G. B. (2007). Applications of fungal ecology in thes for new bioactive natural products. Environmental and microbial relationships. The Mycota, 4, 257–283.
Kendrick, B. (2000). The fifth kingdom (3rd ed.). Newburyport: Focus Publishing, R. Pullins Co.
Lehr, N. A., Meffert, A., Antelo, L., Sterner, O., Anke, H., & Weber, R. W. S. (2006). Antomoebins, myrocin B and the basis of antifungal antibiosis in the coprophilous fungus Stilbella erythrocephala (syn. S. fimetaria). FEMS Microbiology Ecology, 55, 105–112.
Mudur, S. V., Gloer, J. B., & Wicklow, D. T. (2006). Sporminarins A and B: antifungal metabolites from a fungicolous isolate of Sporormiella minimoides. Journal of Antibiotics, 59, 500–506.
Pasricha, R., Kumar, R. N., & Mukerji, K. G. (1994). Succession of coprophilous fungi in relation to chemical composition of the substrate. Ukrainian Botanical Journal, 51, 94–99.
Pretsch, E., Bühlmann, P., & Affolter, C. (2000). Structure determination of organic compounds – tables of spectral data (3rd ed., pp. 161–243). Berlin: Springer.
Richardson, M. J. (2001). Diversity and occurrence of coprophilous fungi. Mycological Research, 15, 387–482.
Ridderbusch, D. C., Weber, R. W. S., Anke, T., & Sterner, O. (2004). Tulasnein and podospirone from the coprophilous xylariaceous fungus Podosordaria tulasnei. Zeitschrift für Naturforschung C. A Journal of Biosciences, 59, 379–383.
Weber, R. W. S., Meffert, A., Anke, H., & Sterner, O. (2005). Production of sordarin and related metabolites by the coprophilous fungus Podospora pleiospora in submerged culture and in its natural substrate. Mycological Research, 109, 619–626.
Wicklow, D. T. (1981). Interference competition and the organization of fungal communities. In D. T. Wicklow & G. C. Carroll (Eds.), The fungal community. Its organization and role in the ecosystem (pp. 351–375). New York: 1′vlarcel Dekker.
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This paper is dedicated to Maurizio Forti, recently passed away.
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Sarrocco, S., Diquattro, S., Avolio, F. et al. Bioactive metabolites from new or rare fimicolous fungi with antifungal activity against plant pathogenic fungi. Eur J Plant Pathol 142, 61–71 (2015). https://doi.org/10.1007/s10658-014-0589-0
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DOI: https://doi.org/10.1007/s10658-014-0589-0