Abstract
Recently, anthracnose has become a major problem in papaya production and postharvest stages. The occurrence of both Colletotrichum gloeosporioides and Colletotrichum capsici has been demonstrated in this crop. The differential response of these pathogens to fungicides has highlighted the need to use rapid and accurate techniques to identify them. Thus, the objective of this study was to reveal the genetic diversity of Colletotrichum isolates in Mexican papaya fields. C. gloeosporioides-and C. capsici-specific primers were successfully used to detect the pathogens from different papaya parts. A combination of morphological characters, molecular techniques and pathogenicity tests were used to characterize 37 isolates from different localities of five papaya-producing states. Analyses of the 5.8-ITS region and arbitrarily primed-PCR revealed intraspecific groups; most of the isolates within these groups have the same geographical location and morphological characteristics. Knowledge of the genetic diversity of Colletotrichum spp. in Mexican papaya fields will facilitate the identification of the pathogen population in this crop in order to select the appropriate fungicide to control anthracnose, as well as to improve genetic resistance breeding programs.
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Abbreviations
- AP-PCR:
-
Arbitrarily primed-polymerase chain reaction
- d. a. i.:
-
Days after inoculation
- ITS:
-
Internal transcribed spacers
References
Adaskaveg, J. E., & Hartin, R. J. (1997). Characterization of Colletotrichum acutatum isolates causing anthracnose of almond and peach in California. Phytopathology, 87, 979–987.
Afanador-Kafuri, L., Minz, D., Maymon, M., & Freeman, S. (2003). Characterization of Colletotrichum isolates from Tamarillo, Passiflora and Mango in Colombia and identification of a unique species from the genus. Phytopathology, 93, 579–587.
Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215, 403–410.
Bailey, J. A., & Jeger, M. J. (1992). Colletotrichum: Biology, pathology and control. Wallingford: CAB.
Cannon, P. F., Bridge, P. D., & Monte, E. (2000). Linking the past, present, and future of Colletotrichum systematics. In D. Prusky, S. Freeman, & M. B. Dickman (Eds.), Colletotrichum host specificity, pathology and host–pathogen interaction (pp. 1–20). St. Paul, Minnesota, USA: The American Phytopathological Society.
Felsestein, J. (1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39, 783–791.
Freeman, S., Katan, T., & Shabi, E. (1998). Characterization of Colletotrichum species responsible for anthracnose diseases of various fruits. Plant Disease, 82, 596–605.
Hammer, Ø., Harper, D. A. T., & Ryan, P. D. (2001). PAST: paleontological statistics software package for education and data analysis. Paleontologia Electronica, 4, 9.
Howard, C. M., Maas, J. L., Chandler, C. K., & Albregts, E. E. (1992). Anthracnose of strawberry caused by the Colletotrichum complex in Florida. Plant Disease, 76, 976–981.
Ihaka, R., & Gentleman, R. (1996). R: a language for data analysis and graphics. Journal of Computational and Graphical Statistics, 5, 299–314.
Johnston, P. R., & Jones, D. (1997). Relationships among Colletotrichum isolates from fruit-rots assessed using rDNA sequences. Mycologia, 89, 420–430.
Kanchana-Udomkan, C., Taylor, P. W. J., & Mongkolporn, O. (2004). Development of a bioassay to study anthracnose infection of Capsicumm chinense Jacq. fruit caused by Colletotrichum capsici. Thai Journal of Agriculture Science, 37, 293–297.
Mahuku, G. S., & Riascus, J. J. (2004). Virulence and molecular diversity within Colletotrichum lindemuthianum isolates from Andean and Mesoamerican bean varieties and regions. European Journal of Plant Pathology, 110, 253–263.
Mills, P., Sreenivasaprasad, S., & Brown, A. E. (1992). Detection and differentiation of Colletotrichum gloeosporioides isolates using PCR. FEMS Microbiology Letters, 98, 137–143.
Nguyen, T. H. P., Säll, T., Bryngelsson, T., & Liljeroth, E. (2009). Variation among Colletotrichum gloeosporioides isolates from infected coffee berries at different locations in Vietnam. Plant Pathology, 58, 898–909.
Photita, W., Taylor, P. W. J., Ford, R., Hyde, K. D., & Lumyong, S. (2005). Morphological and molecular characterization of Colletotrichum species from herbaceous plants in Thailand. Fungal Diversity, 18, 117–133.
Rampersad, S. N. (2011). Molecular and phenotypic characterization of Colletotrichum species associated with anthracnose disease of papaya in Trinidad. Plant Disease, 95, 1244–1254.
Sangdee, A., Sachan, S., & Khankhum, S. (2011). Morphological, pathological and molecular variability of Colletotrichum capsici causing anthracnose of chilli in the North-east of Thailand. African Journal of Microbiology Research, 5, 4368–4372.
Schiller, M., Lübeck, M., Sundelin, T., Campos Meléndez, L. F., Danielsen, S., Jensen, D. F., & Madriz Ordeñana, K. (2006). Two subpopulations of Colletotrichum acutatum are responsible for anthracnose in strawberry and leatherleaf fern in Costa Rica. European Journal of Plant Pathology, 116, 107–118.
Shenoy, B. D., Jeewon, R., Lam, W. H., Bhat, D. J., Than, P. P., Taylor, P. W. J., & Hyde, K. D. (2007). Morpho-molecular characterization and epitypification of Colletotrichum capsici (Glomerellaceae, Sordariomycetes), the causative agent of anthracnose in chilli. Fungal Diversity, 27, 197–211.
Silva-Rojas, H. V., & Avila-Quezada, G. D. (2011). Phylogenetic and morphological identification of Colletotrichum boninense: a novel causal agent of anthracnose in avocado. Plant Pathology, 60, 899–908.
Sreenivasaprasad, S., & Talhinhas, P. (2005). Genotypic and phenotypic diversity in Colletotrichum acutatum, a cosmopolitan pathogen causing anthracnose on a wide range of hosts. Molecular Plant Pathology, 6, 361–378.
Sutton, B. C. (1992). The genus Glomerella and its anamorph Colletotrichum. In J. A. Bailey & J. J. Jeger (Eds.), Colletotrichum: biology, pathology and control (pp. 1–26). Wallingford: CAB.
Talhinhas, P., Sreenivasaprasad, S., Neves-Martins, J., & Oliveira, H. (2005). Molecular and phenotypic analyses reveal association of diverse Colletotrichum acutatum groups and a low level of C. gloeosporioides with olive anthracnose. Applied and Environmental Microbiology, 71, 2987–2998.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., & Kumar, S. (2011). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance and maximum parsimony methods. Molecular Biology and Evolution. Online [http://mbe.oxfordjournals.org/].
Tapia-Tussell, R., Lappe, P., Ulloa, M., Quijano-Ramayo, A., Caceres-Farfan, M., Larque-Saavedra, A., & Perez-Brito, D. (2006). A rapid and simple method for DNA extraction from yeasts and fungi isolated from Agave fourcroydes. Molecular Biotechnology, 33, 67–69.
Tapia-Tussell, R., Quijano-Ramayo, A., Cortes-Velazquez, A., Lappe, P., Larque-Saavedra, A., & Perez-Brito, D. (2008). PCR-based detection and characterization of the fungal pathogens Colletotrichum gloeosporioides and Colletotrichum capsici causing anthracnose in papaya (Carica papaya L.) in the Yucatan Peninsula. Molecular Biotechnology, 40, 293–298.
Tarnowski, T. L. B., & Ploetz, R. C. (2010). First report of Colletotrichum capsici causing postharvest anthracnose on papaya in South Florida. Plant Disease, 94, 1065.
Than, P. P., Jeewon, R., Hyde, K. D., Pongsupasamit, S., Mongkolporn, O., & Taylor, P. W. J. (2008). Characterization and pathogenicity of Colletotrichum species associated with anthracnose disease on chilli (Capsicum spp.) in Thailand. Plant Pathology, 57, 562–572.
Torres-Calzada, C., Tapia-Tussell, R., Quijano-Ramayo, A., Martin-Mex, R., Rojas-Herrera, R., Higuera-Ciapara, I., & Perez-Brito, D. (2011). A species-specific polymerase chain reaction assay for rapid and sensitive detection of Colletotrichum capsici. Molecular Biotechnology, 49, 48–55.
Torres-Calzada, C., Tapia-Tussell, R., Nexticapan-Garcez, A., Matin-Mex, R., Quijano-Ramayo, A., Cortés-Velázquez, A., Higuera-Ciapara, I., & Perez-Brito, D. (2011). First report of Colletotrichum capsici causing anthracnose in Jatropha curcas in Yucatan, Mexico. New Disease Reports, 23, 6.
Van Hemelrijck, W., Debode, J., Heungens, K., Maes, M., & Creemers, P. (2010). Phenotypic and genetic characterization of Colletotrichum isolates from Belgian strawberry fields. Plant Pathology, 59, 853–861.
White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M. A. Innis, D. H. Gelfand, J. J. Shinsky, & T. J. White (Eds.), PCR protocols: A guide to methods and applications (pp. 315–322). San Diego: Academic.
Whitelaw-Weckert, M. A., Curtin, S. J., Huang, R., Steel, C. C., Blanchard, C. L., & Roffey, P. E. (2007). Phylogenetic relationships and pathogenicity of Colletotrichum acutatum isolates from grape in subtropical Australia. Plant Pathology, 56, 448–463.
Acknowledgments
This research was supported by the Quintana Roo Produce Foundation through Grant 23-2009-1712. The authors thank Rodolfo Martin-Mex and Angel Nexticapan-Garcez for their valuable help in sample collection. Anuar Magaña-Alvarez and Gamaliel Itza-Kuk are thanked for their excellent technical assistance.
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Torres-Calzada, C., Tapia-Tussell, R., Higuera-Ciapara, I. et al. Morphological, pathological and genetic diversity of Colletotrichum species responsible for anthracnose in papaya (Carica papaya L). Eur J Plant Pathol 135, 67–79 (2013). https://doi.org/10.1007/s10658-012-0065-7
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DOI: https://doi.org/10.1007/s10658-012-0065-7