Skip to main content
SpringerLink
Log in

Interactive effects of simultaneous ozone and fluoranthene fumigation on the eco-physiological status of the evergreen conifer, Japanese red pine (Pinus densiflora Sieb et. Zucc.)

  • Published:
Ecotoxicology Aims and scope Submit manuscript

Abstract

Forest decline has long been attributed to air pollution and acid rain/fog, with ozone having a record for damaging trees. This study investigated eco-physiological changes on Japanese red pine caused by simultaneous fumigation of O3 (O) and fluoranthene (F) over a 90 day period. Seedlings were exposed individually or in combinations to 10 μM fluoranthene and O3 (3 ppm and 6 ppm in 60 days and 90 days, respectively) inside growth chambers. Eco-physiological parameters monitored included gas exchange, chlorophyll fluorescence, needle chlorophyll content, and visual appearance. After 90 days, O + F treatment showed deleterious effects on visual needle appearance and the net photosynthesis rate near saturated irradiance. In addition, decreased levels in stomatal conductance, photochemical efficiency of PS II in the dark, and total chlorophyll and Chl a: Chl b were observed. F only treatment showed similar results but in lesser magnitude compared with F + O treatment. O treatment alone showed no significant negative effect, probably due to its low concentration in the 60 day treatment. The addition of mannitol (OH radical scavenger) mitigated O + F and F negative effects. Fluoranthene deposited on Japanese red pine presents great eco-physiological damage risk, even at low O3 concentration. Furthermore, the effects of O3 assisted phyto-toxicity of fluoranthene on red pine may have relevance to other plant species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Adams MB, Kelly JM, Edwards NT (1988) Growth of Pinus taeda L seedlings varies with family and ozone exposure level. Water Air Soil Pollut 38:137–150

    CAS  Google Scholar 

  • Bergmann E, Bender J, Weigel HJ (1999) Ozone threshold doses and exposure-response relationships for the development of ozone injury symptoms in wild plant species. New Phytol 144:432–435. doi:10.1046/j.1469-8137.1999.00534.x

    Article  Google Scholar 

  • Edwards NT, Edwards GL, Kelly JM, Taylor EG Jr (1992) Three-year growth response of Pinus Taeda L to simulated rain chemistry soil magnesium status and ozone. Water Air Soil Pollut 63:105–118. doi:10.1007/BF00475625

    Article  CAS  Google Scholar 

  • Fangmeier A, Bender J, Weigel HJ, Jager HJ (2002) Effects of pollutants mixtures. In: Bell JN, Treshow M (eds) Air pollution and plant life, 2nd edn. ISBN 0417490903 (HB). Wiley, 251–269

  • Farage PK, Long SP, Lechner EG, Baker NR (1991) The sequence of change within the photosynthetic apparatus of wheat following short time exposure to ozone. Plant Physiol 95:529–535

    Article  CAS  Google Scholar 

  • Finlayson-Pitts B, Pitts NJ Jr (1993) Atmospheric chemistry of tropospheric ozone formation: scientific and regulatory implications. Air Waste 43:1091–1100

    CAS  Google Scholar 

  • Huang XD, Lorelei FZ, Dixon DG, Greenberg BM (1996) Photoinduced toxicity of PAHs to the foliar regions of Brassica napus (Canola) and Cucumis sativus (Cucumber) in simulated solar radiation. Ecotoxicol Environ Saf 35:190–197. doi:10.1006/eesa.1996.0099

    Article  CAS  Google Scholar 

  • Huang XD, McConkey BJ, Babu TS, Greenberg BM (1997) Mechanism of photoinduced toxicity of photomodified anthracene to plants: inhibition of photosynthesis in the aquatic higher plant Lemna gibba (duckweed). Environ Toxicol Chem 8:1707–1715. doi :10.1897/1551-5028(1997)016<1707:MOPTOP>2.3.CO;2

    Article  Google Scholar 

  • Izuta T (2001) Plant responses to atmospheric change (in Japanese) (‘Youken-dou’ edited and written by Nouchi I.), pp 168–208

  • Keller T, Hasler R (1987) Some effects of long-term ozone fumigations on Norway spruce. Trees (Berl) 1:129–133

    Google Scholar 

  • Keller T, Landolt W (1985) Uptake and effects of air pollutants on woody plants. Birkhauser Verlag, CH-4010 Basel/Switzerland. Experientia 41:301–310. doi:10.1007/BF02004490

    Article  Google Scholar 

  • Kerstiens G, Lendzian KJ (1989) Interactions between ozone and plant cuticles. I. Ozone deposition and permeability. New Phytol 112:13–19. doi:10.1111/j.1469-8137.1989.tb00303.x

    Article  CAS  Google Scholar 

  • Kohno Y (2005) Global Environment Research Fund, Ministry of the Environment, C-7 Study for assessment of vegetation effects of acid and oxidizing substances and establishment of critical levels in East Asia (in Japanese)

  • Kohno Y, Matsumura H, Ishii T, Izuta T (2005) Establishing critical levels of air pollutants for protecting East Asian vegetation—a challenge. In: Omasa K, Nouchi I, De Kok LJ (eds) Plant responses to air pollution and global change. Springer-Verlag, Tokyo, pp 243–250

    Chapter  Google Scholar 

  • Kummerova M, Kmentova E (2004) Photoinduced toxicity of fluoranthene on germination and early development of plant seedling. Chemosphere 56:387–393. doi:10.1016/j.chemosphere.2004.01.007

    Article  CAS  Google Scholar 

  • Kummerová M, Barták M, Dubová J, Třıíska J, Zubrová E, Zezulka S (2006a) Inhibitory effect of fluoranthene on photosynthetic processes in lichens detected by chlorophyll fluorescence. Ecotoxicology 15:121–131. doi:10.1007/s10646-005-0037-1

    Article  CAS  Google Scholar 

  • Kummerová M, Krulová J, Zezulka S, Tříska J (2006b) Evaluation of fluoranthene phytotoxicity in pea plants by Hill reaction and chlorophyll fluorescence. Chemosphere 65:489–496. doi:10.1016/j.chemosphere.2006.01.052

    Article  CAS  Google Scholar 

  • Lee CL, Huang HT, Kuo LJ (2000) Experimental validation of an OMS model for the sorption behaviors of PAHs onto aluminium oxide coated with humic acids. J Environ Sci Health A 25:515–536

    Article  Google Scholar 

  • Mallakin A, Babu TS, Dixon DG, Greenberg BM (2002) Sites toxicity of specific photooxidation products of anthracene to higher plants: inhibition of photosynthetic activity and electron transport in Lemna gibba L. G-3 (Duckweed). Environ Toxicol 17:462–471. doi:10.1002/tox.10080

    Article  CAS  Google Scholar 

  • Nakaji T, Fukami M, Dokiya Y, Izuta T (2001) Effect of high nitrogen load on growth, photosynthesis and nutrient status of Cryptomeria japonica and Pinus densiflora seedlings. Trees (Berl) 15:453–461

    CAS  Google Scholar 

  • Nakaji T, Kobayashi T, Kuroha M, Omori K, Yonekura T, Matsumoto , Watanabe K, Utrainen J, Izuta T (2004) Growth and nitrogen availability of red pine seedlings under high nitrogen load and elevated ozone. Water Air Soil Pollut Focus 4:277–287. doi:10.1023/B:WAFO.0000028360.61672.8d

    Article  CAS  Google Scholar 

  • Nakane K, Sakugawa H, Igawa M (2000) Air pollution and tree damages in the pine or fir forest decline area in Hiroshima or Kanagawa Prefecture, Japan. Jpn J Ecol 50:319–324

    Google Scholar 

  • Oguntimehin I, Nakatani N, Sakugawa H (2007) Phytotoxicities of fluoranthene and phenanthrene deposited on needle surfaces of the evergreen conifer Japanese red pine (Pinus densiflora Sieb et. Zucc). Environ Pollut. doi:10.1016/j.evol.2007.10.039

  • Porra RJ, Thompson WA, Kriedemann PE (1989) Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochim Biophys Acta 975:384–394. doi:10.1016/S0005-2728(89)80347-0

    Article  CAS  Google Scholar 

  • Schraudner M, Moeder W, Wiese C, Van Camp W, Inze D, Lagebartels C, Sandermann H (1998) Ozone-induced oxidative burst in the ozone biomonitor plant, tobacco Bel W3. Plant J 16:235–245. doi:10.1046/j.1365-313x.1998.00294.x

    Article  CAS  Google Scholar 

  • Shimizu H, Feng YW (2007) Ozone and/or water stress could have influenced the Betula ermanii Cham. Forest decline observed at Oku-Nikko, Japan. Environ Monit Assess 128:109–119. doi:10.1007/s10661-006-9409-0

    Article  CAS  Google Scholar 

  • Takagi K, Ohara T (2003) Estimation of ozone impact on plants by damage functions in the Kanto area. J Jpn Soc Atmos Environ 38:205–206; in Japanese

    CAS  Google Scholar 

  • Thompson AM (1992) The oxidizing capacity of the earth’s atmosphere: probable past and future changes. Science 256:1157–1165. doi:10.1126/science.256.5060.1157

    Article  CAS  Google Scholar 

  • Wakamastu S, Uno I, Ueda H, Uehara K, Tateishi H (1989) Observational study of stratospheric ozone instructions into the lower troposphere. Atmos Environ 23:1815–1826. doi:10.1016/0004-6981(89)90065-6

    Article  Google Scholar 

  • Wild SR, Jones KC (1991) Studies on the polynuclear aromatic hydrocarbon content of carrots (Daucus carota). Chemosphere 23:243–251. doi:10.1016/0045-6535(91)90110-Y

    Article  CAS  Google Scholar 

  • Wild SR, Jones KC (1992) Polynuclear aromatic hydrocarbon uptake by carrots grown in sludge-amended soil. J Environ Qual 21:217–225

    Article  CAS  Google Scholar 

  • Wild E, Dent J, Thomas GO, Jones KC (2006) Visualizing the air-to-leaf transfer and within-leaf movement and distribution of phenanthrene: further studies utilizing two-photon excitation microscopy. Environ Sci Technol 40:907–916. doi:10.1021/es0515046

    Article  Google Scholar 

  • Zinser C, Jungblut T, Heller W, Seidlitz HK, Schnitzler JP, Ernst D, Sadermann H Jr (2000) The effects of ozone ii scot pine (Pinus sylvestris L.): gene expression, biochemical changes and interactions with UV-B radiation. Plant Cell Environ 23:975–982. doi:10.1046/j.1365-3040.2000.00613.x

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Environmental Dynamics and Management, Graduate School of Biosphere Science, Hiroshima University, 1-7-1 Kagamiyama, Higashi-Hiroshima, 739-8521, Japan

    Ilemobayo Oguntimehin & Hiroshi Sakugawa

Authors
  1. Ilemobayo Oguntimehin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hiroshi Sakugawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hiroshi Sakugawa.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Oguntimehin, I., Sakugawa, H. Interactive effects of simultaneous ozone and fluoranthene fumigation on the eco-physiological status of the evergreen conifer, Japanese red pine (Pinus densiflora Sieb et. Zucc.). Ecotoxicology 18, 100–109 (2009). https://doi.org/10.1007/s10646-008-0263-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10646-008-0263-4

Keywords

Search

Navigation