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Highly polymorphic microsatellite markers for the highly polymorphic strawberry poison-dart frog and some of its congeners

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Abstract

Members of the poison-dart frog genus Oophaga, including the strawberry poison-dart frog (O. pumilio) display among the most striking examples of color polymorphism of any amphibians. We developed twelve novel microsatellite markers with di-, tri-, and tetra-nucleotide repeats for this genus. These loci are highly polymorphic with between 2 and 29 alleles (average = 14.7) and high heterozygosity (H O = 0.704). These highly polymorphic markers should be useful for resolving fine-scale genetic differences between the different color morphs of these highly variable species, for investigations into the ecological importance of this variation, and for determining the effects of habitat loss and fragmentation on population persistence in these species.

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Acknowledgments

We thank HB Shaffer, RC Thomson, JR Johnson, E Twomey, J Yeager, JL Brown, and T Kosch for advice on this project, which was conducted with support from the UC Davis Center for Population Biology and an NSF Graduate Research Fellowship (to IJW) and a Research and Creative Activity Grant from East Carolina University (to KS).

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Authors and Affiliations

  1. Center for Population Biology, Department of Evolution and Ecology, University of California, 1 Shields Ave, Davis, CA, 95616, USA

    Ian J. Wang

  2. Department of Biology, East Carolina University, East Fifth St., Greenville, NC, 27858, USA

    Kyle Summers

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  1. Ian J. Wang
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  2. Kyle Summers
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Correspondence to Ian J. Wang.

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Wang, I.J., Summers, K. Highly polymorphic microsatellite markers for the highly polymorphic strawberry poison-dart frog and some of its congeners. Conserv Genet 10, 2033–2036 (2009). https://doi.org/10.1007/s10592-009-9887-z

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  • DOI: https://doi.org/10.1007/s10592-009-9887-z

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