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Isolation and characterization of microsatellite markers for the endangered Monimopetalum chinense (Celastraceae)

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Abstract

We describe the isolation and characterization of 13 polymorphic microsatellite loci in the endangered Monimopetalum chinense (Celastraceae) using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol in this paper. The 13 loci were tested in 20 individuals from two populations. The number of alleles per locus varied from 2 to 8, with an average of 3.8. The expected and observed heterozygosities ranged from 0.05 to 0.87 and from 0.05 to 0.55, respectively. Two of the 13 loci were biased from Hardy–Weinberg equilibrium. No significant linkage disequilibrium was found among all loci. These polymorphic SSR markers will be useful in studying population genetics of M. chinense.

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Acknowledgments

We are grateful to Shen-Min Guan, Yan Chen, Min Liu, Miao-Miao Shi, Mei-Hua Liu, Rong Wang and Lin-Feng Li for their technical assistants. This work was supported by a grant from the National Natural Science Foundation of China (30470146) and Program for 211 Project and New Century Excellent Talents in University (NCET-05-0431).

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Authors and Affiliations

  1. Department of Environmental Sciences, East China Normal University, 200062, Shanghai, China

    Hai-Sheng Li & Xiao-Yong Chen

  2. Department of Biology, Guangdong Education Institute, 510303, Guangzhou, China

    Hai-Sheng Li

  3. College of Life Sciences, Guangzhou University, 510006, Guangzhou, China

    Guo-Wen Xie

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  1. Hai-Sheng Li
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  2. Guo-Wen Xie
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  3. Xiao-Yong Chen
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Correspondence to Xiao-Yong Chen.

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Li, HS., Xie, GW. & Chen, XY. Isolation and characterization of microsatellite markers for the endangered Monimopetalum chinense (Celastraceae). Conserv Genet 10, 1497–1499 (2009). https://doi.org/10.1007/s10592-008-9767-y

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  • DOI: https://doi.org/10.1007/s10592-008-9767-y

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