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LALF32-51-E7 therapeutic vaccine induces antitumor immunity against human papillomavirus type 16 E7-expressing murine tumor metastases in the lungs

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Abstract

One important goal of cancer immunotherapy is to prevent and treat tumor metastasis. We have previously reported the significant antitumor effect induced by the immunization with our human papillomavirus therapeutic protein-based vaccine (LALF32-51-E7) without adjuvant and admixed with clinically relevant adjuvants in the subcutaneous TC-1 tumor challenge model. In the present study, we evaluated the efficacy of the above mentioned vaccine formulations in controlling the hematogenous spread of TC-1 tumor cells using a more tumourigenic clone named TC-1* and other intravenous injection site less stressful than the tail vein. We generated a lung metastasis model by injecting TC-1* cells into the retro-orbital venous sinus and this is the first study describing it. Also, this is the first study that demonstrates the efficacy of the immunization with LALF32-51-E7 without adjuvant and admixed with VSSP or Al(OH)3 in controlling metastatic tumors increasing the survival of the mice. Our TC-1 lung metastasis model can be used to test the efficacy of other immunotherapeutic strategies based on E6/E7 antigens.

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Abbreviations

HPV:

Human papillomavirus

s.c:

Subcutaneous

i.v:

Intravenous

VSSP:

Very small size proteoliposomes

Al(OH)3 :

Aluminium hydroxide

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Acknowledgements

This research was supported by the Center for Genetic Engineering and Biotechnology, Havana, Cuba.

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Authors and Affiliations

  1. Department of Pharmaceuticals, Center for Genetic Engineering and Biotechnology, P.O. Box 6162, Cubanacan, Playa, 10600, Havana, Cuba

    Milaid Granadillo, Aileen Batte, Alain B. Alfonso & Isis Torrens

  2. Animal Care Unit, Center for Genetic Engineering and Biotechnology, P.O. Box 6162, Cubanacan, Playa, 10600, Havana, Cuba

    Aracelys Blanco, José Suárez, Rosalina Carballo, Bárbara O. González & Lizet Aldana

  3. Center for Pharmaceutical Research and Drug Development, Plaza, 10400, Havana, Cuba

    Nelson Merino

  4. Technological Development Unit, Center for Genetic Engineering and Biotechnology, P.O. Box 6162, Cubanacan, Playa, 10600, Havana, Cuba

    Yayrí C. Prieto, Laura Varas, Dayana Soler, Miladys Limonta & Maelys Miyares

Authors
  1. Milaid Granadillo
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  2. Aileen Batte
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  3. Aracelys Blanco
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  4. Alain B. Alfonso
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  5. José Suárez
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  6. Nelson Merino
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  7. Rosalina Carballo
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  8. Bárbara O. González
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  9. Yayrí C. Prieto
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  10. Laura Varas
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  11. Dayana Soler
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  12. Miladys Limonta
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  13. Maelys Miyares
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  14. Lizet Aldana
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  15. Isis Torrens
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Corresponding author

Correspondence to Milaid Granadillo.

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The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving animals were approved by the Animal Care and Use Committee at the Center for Genetic Engineering and Biotechnology, Cuba.

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Granadillo, M., Batte, A., Blanco, A. et al. LALF32-51-E7 therapeutic vaccine induces antitumor immunity against human papillomavirus type 16 E7-expressing murine tumor metastases in the lungs. Clin Exp Metastasis 34, 241–249 (2017). https://doi.org/10.1007/s10585-017-9846-x

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  • DOI: https://doi.org/10.1007/s10585-017-9846-x

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