Abstract
Objectives
To investigate the transdifferentiation of bone marrow-derived mesenchymal stem cells (BMSCs) into salivary gland-like cells via a novel culture method employing induction culture medium collected from salivary gland cells.
Results
Primary salivary gland cells were cultured, and after the first passage, the culture medium was collected for use as induction medium. BMSCs (passage 3) were cultured in either induction medium (induction group) or DMEM/F12 medium with 10 % (v/v) fetal bovine serum (control group) before seeding on three-dimensional collagen/chitosan scaffolds and subcutaneous transplantation into nude mice. The in vitro and in vivo transdifferentiation of BMSCs into salivary gland-like cells was evaluated by immunocytochemical analysis of α-amylase and cytokeratin-8 (CK-8) expression. Salivary gland-like cells cultured using this novel method maintained excellent biostability and exhibited relatively stable expression of α-amylase and CK-8 in vitro and in vivo.
Conclusion
This novel culture method is feasible for inducing the transdifferentiation of BMSCs into salivary gland-like cells.
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Acknowledgments
We thank Medjaden Bioscience Limited for assisting in the preparation of this manuscript. This study was supported by Natural Science Foundation of China 81072230 and 30772428.
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The authors have declared that no competing interests exist.
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Liang Liang and Jun Wang have contributed equally to this work.
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Liang, L., Wang, J., Zhang, Y. et al. Transdifferentiation of bone marrow-derived mesenchymal stem cells into salivary gland-like cells using a novel culture method. Biotechnol Lett 37, 1505–1513 (2015). https://doi.org/10.1007/s10529-015-1809-1
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DOI: https://doi.org/10.1007/s10529-015-1809-1