Abstract
Analysis of the structural properties of pYC2, a cryptic plasmid from Lactobacillus sakei BM5 isolated from kimchi, determined its length as 1,970 bp with a G + C content of 34%. The double-strand origin (dso) and single-strand origin (sso) of rolling-circle replicating plasmids were found in the nucleotide sequence of the pYC2 plasmid. Sequence analysis of pYC2 revealed that ORF 1 and ORF 2 showed high homology with the Cop and Rep proteins encoded by the pMV158 family of plasmids replicating via the rolling-circle mechanism. pYC2 also replicates by this mechanism, as confirmed by Southern hybridization analysis.
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Acknowledgment
This work was supported by grant number RTI05-01-01 from the Regional Technology Innovation Program of the Ministry of Knowledge Economy (MKE), Republic of Korea.
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Yang, E.J., Chang, H.C. Analysis of pYC2, a cryptic plasmid in Lactobacillus sakei BM5 isolated from kimchi. Biotechnol Lett 31, 123–130 (2009). https://doi.org/10.1007/s10529-008-9842-y
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DOI: https://doi.org/10.1007/s10529-008-9842-y