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Genetic diversity of Ralstonia solanacearum strains causing bacterial wilt of solanaceous crops in Myanmar

  • Bacterial and Phytoplasma Diseases
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Abstract

In 2013 and 2014, an extensive survey of bacterial wilt in Myanmar was performed, and 70 strains of Ralstonia solanacearum (Rs) were collected from wilting plants of tomato, potato, chili and eggplant. Myanmar Rs strains were characterized by traditional and molecular methods. Polymerase chain reaction (PCR) test using Rs-specific primer set amplified one specific band (281-bp) from template DNA of all strains. Pathogenicity tests on the four solanaceous plants differentiated the strains into six pathogenic groups. Biovar determination tests showed that biovar 3 strains predominated (63%) among all Rs strains. Biovar 4 strains (7%) were obtained from both tomato and chili strains, whereas biovar 2 (30%) strains were isolated only from potato. Multiplex-PCR analysis indicated that tomato, eggplant and chili strains belonged to phylotype I, whereas potato strains comprised phylotype I and phylotype II. Strains in phylotype I, which was suggested to have originated from Asia, were the most prevalent in all surveyed areas. Phylogenetic analysis based on the endoglucanase (egl) gene sequences revealed that Myanmar strains partitioned into two major clusters that corresponded to phylotype I and II. Strains in phylotype I were further divided into seven subclusters, each corresponding to a distinct sequevar (15, 17, 46, 47, 48, unknown 1 or unknown 2). All strains in phylotype II belonged to sequevar 1. This is the first comprehensive report of the presence of diverse Rs strains in Myanmar.

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References

  • Buddenhagen I, Kelman A (1964) Biological and physiological aspects of bacterial wilt caused by Pseudomonas solanacearum. Annu Rev Phytopathol 2:203–230

    Article  Google Scholar 

  • Buddenhagen I, Sequeria L, Kelman A (1962) Designation of races of Pseudomonas solanacearum. Phytopathology 52:726 (Abstract)

    Google Scholar 

  • Castillo JA, Greenberg JT (2007) Evolutionary dynamics of Ralstonia solanacearum. Appl Environ Microbiol 73:1225–1238

    Article  CAS  PubMed  Google Scholar 

  • Cellier G, Prior P (2010) Deciphering phenotypic diversity of Ralstonia solanacearum strains pathogenic to potato. Phytopathology 100:1250–1261

    Article  CAS  PubMed  Google Scholar 

  • Elphinstone JG (2005) The current bacterial wilt situation: a global overview. In: Allen C, Prior P, Hayward AC (eds) Bacterial wilt: the disease and the Ralstonia solanacearum species complex. APS Press, St. Paul, pp 9–28

    Google Scholar 

  • Fegan M, Prior P (2005) How complex is the "Ralstonia solanacearum species complex". In: Allen C, Prior P, Hayward AC (eds) Bacterial wilt disease and the Ralstonia solanacearum species complex. APS Press, St. Paul, pp 449–461

    Google Scholar 

  • Hayward AC (1964) Characteristics of Pseudomonas solanacearum. J Appl Bacteriol 27:265–277

    Article  Google Scholar 

  • Hayward AC (1991) Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum. Annu Rev Phytopathol 29:65–87

    Article  CAS  PubMed  Google Scholar 

  • Hayward AC (1994) The hosts of Pseudomonas solanacearum. In: Hayward AC, Hartman GL (eds) Bacterial wilt: the disease and its causative agent, Pseudomonas solanacearum. CAB International, Wallingford, pp 9–24

    Google Scholar 

  • Hayward AC, Elphinstone JG, Caffier D, Janse JD, Stefani E, French ER, Wright AJ (1998) Round table on bacterial wilt (brown rot) of potato. In: Prior PH, Allen C, Elphinstone JG (eds) Bacterial wilt disease: molecular and ecological aspects. Springer, Heidelberg, pp 420–430

    Chapter  Google Scholar 

  • He LY, Sequiera L, Kelman A (1983) Characteristics of strains of Pseudomonas solanacearum from China. Plant Dis 67:1357–1361

    Article  Google Scholar 

  • Horita M, Tsuchiya K (2001) Genetic diversity of Japanese strains of Ralstonia solanacearum. Phytopathology 91:399–407

    Article  CAS  PubMed  Google Scholar 

  • Horita M, Tsuchiya K, Ooshiro A (2005) Characteristics of Ralstonia solanacearum Biovar N2 Strains in Asia. J Phytopathol 153:209–213

    Article  Google Scholar 

  • Horita M, Suga Y, Ooshiro A, Tsuchiya K (2010) Analysis of genetic and biological characters of Japanese potato strains of Ralstonia solanacearum. J Gen Plant Pathol 76:196–207

    Article  CAS  Google Scholar 

  • Horita M, Tsuchiya K, Suga Y, Yano K, Waki T, Kurose D, Furuya N (2014) Current classification of Ralstonia solanacearum and genetic diversity of the strains in Japan. J Gen Plant Pathol 80:455–465

    Article  CAS  Google Scholar 

  • Huang Q, Xuerui Y, Jaw FW (2011) Improved biovar test for Ralstonia solanacearum. J Microbiol Methods 88:271–274

    Article  PubMed  Google Scholar 

  • Janse JD (1991) Infra- and intraspecific classification of Pseudomonas solanacearum strains, using whole cell fatty acid analysis. Syst Appl Microbiol 14:335–345

    Article  Google Scholar 

  • Janse JD (1996) Potato brown rot in Western Europe—History, presence occurrence and some remarks on possible origin, epidemiology and control strategies. EPPO Bull 26:679–985

    Article  Google Scholar 

  • Jeong Y, Kim J, Kang Y, Lee S, Hwang I (2007) Genetic diversity and distribution of Korean isolates of Ralstonia solanacearum. Plant Dis 91:1277–1287

    Article  CAS  Google Scholar 

  • Ji P, Allen C, Sanchez-Perez A, Yao J, Elphinstone JG, Jones JB, Momol MT (2007) New diversity of Ralstonia solanacearum strains associated with vegetable and ornamental crops in Florida. Plant Dis 91:195–203

    Article  CAS  Google Scholar 

  • Kelman A (1953) The bacterial wilt caused by Pseudomonas solanacearum. A literary review and bibliography. Tech Bull North Carol Agric Exp Stn 99:1–194

    Google Scholar 

  • Kelman A (1954) The relationship of pathogenicity in Pseudomonas solanacearum to colony appearance on a tetrazolium chloride medium. Phytopathology 44:693–695

    Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    Article  CAS  PubMed  Google Scholar 

  • Lewis Ivey ML, McSpadden Gardener BB, Opina N, Miller SA (2007) Diversity of Ralstonia solanacearum infecting eggplant in the Philippines. Phytopathology 97:1467–1475

    Article  Google Scholar 

  • Liu Y, Kanda Y, Yano K, Kiba A, Hikichi Y, Aino M, Kawaguchi A, Mizoguchi S, Nakaho K, Shiomi H, Takikawa Y, Ohnishi K (2009) Molecular typing of Japanese strains of Ralstonia solanacearum in relation to the ability to induce a hypersensitive reaction in tobacco. J Gen Plant Pathol 75:369–380

    Article  CAS  Google Scholar 

  • Lwin M (2006) Biological control of bacterial wilt disease through antagonistic microbial populations. Doctor of Technical Science dissertation, Asian Institute Technology, Bangkok, Thailand

  • Mahbou Somo Toukam G, Cellier G, Wicker E, Guilhaud C, Kahane R, Allen C, Prior P (2009) Broad diversity of Ralstonia solanacearum strains in Cameroon. Plant Dis 93:1123–1130

    Article  Google Scholar 

  • Marin JE, El-Nashaar HM (1993) Pathogenicity and new phenotypes of Pseudomonas solanacearum from Peru. In: Hartman GL, Hayward AC (eds) Bacterial wilt, vol 45. ACIAR Proceedings, Canberra, pp 78–84

    Google Scholar 

  • MAS (Myanmar Agricultural Services) and the International Potato Center (CIP) (1990) Potato research and development in Union of Myanmar (1983–1989), Rangoon and Manilla, pp 1–47

  • MOAI (Ministry of Agriculture and Irrigation) (2014) Myanmar agriculture at a glance. Department of Agricultural Planning, Myanmar, pp 6–7

    Google Scholar 

  • Myint MM (2001) Potato late blight in Myanmar. Proceedings of the East and Southeast Asia Linkage Group, Baoding, China. J Agric Univ Hebei 24:24–26

    Google Scholar 

  • Nouri S, Bahar M, Fegan M (2009) Diversity of Ralstonia solanacearum causing potato bacterial wilt in Iran and the first record of phylotype II/biovar 2T strains outside South America. Plant Pathol 58:243–249

    Article  Google Scholar 

  • Opina N, Tavner F, Hollway G, Wang JF, Li TH, Maghirang R, Fegan M, Hayward AC, Krishnapillai V, Hong WF, Holloway BW, Timmins J (1997) A novel method for development of species and strain-specific DNA probes and PCR primers for identifying Burkholderia solancearum (formerly Pseudomonas solancearum). Asia Pac J Mol Biol Biotechnol 5:19–30

    Google Scholar 

  • Pegg KG, Moffett ML (1971) Host range of the ginger strain of Pseudomonas solanacearumin Queensland. Aust J Exp Agric Anim Husb 11:696–698

    Article  Google Scholar 

  • Poussier S, Prior P, Luisetti J, Hayward C, Fegan M (2000) Partial sequencing of the hrpB and endoglucanase genes confirms and expands the known diversity within the Ralstonia solanacearum species complex. Syst Appl Microbiol 23:479–486

    Article  CAS  PubMed  Google Scholar 

  • Prior P, Fegan M (2005) Recent developments in the phylogeny and classification of Ralstonia solanacearum. Acta Hort 695:127–136

    Article  CAS  Google Scholar 

  • Sagar V, Jeevalatha A, Mian S, Chakrabarti SK, Gurjar MS, Arora RK, Sharma S, Bakade RR, Singh BP (2013) Potato bacterial wilt in India caused by strains of phylotype I, II and IV of Ralstonia solanacearum. Eur J Plant Pathol 138:51–65

    Article  Google Scholar 

  • Smith JJ, Offord LC, Holderness M, Saddler GS (1995) Genetic diversity of Burkholderia solanacearum (synonym Pseudomonas solanacearum) race 3. Kenya. Appl Environ Microbiol 61:4263–4268

    CAS  PubMed  PubMed Central  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tsuchiya K (2014) Genetic diversity of Ralstonia solanacearum and disease management strategy. J Gen Plant Pathol 80:504–509

    Article  Google Scholar 

  • Villa JE, Tsuchiya K, Horita M, Natural M, Opina N, Hyakumachi M (2005) Phylogenetic relationships of Ralstonia solanacearum species complex strains from Asia and other continents based on 16 S rDNA, endoglucanase, and hrpB gene sequences. J Gen Plant Pathol 71:39–46

    Article  CAS  Google Scholar 

  • Wicker E, Lefeuvre P, de Cambiaire J-C, Lemaire C, Poussier S, Prior P (2012) Contrasting recombination patterns and demographic histories of the plant pathogen Ralstonia solanacearum inferred from MLSA. ISME J 6:961–974

    Article  CAS  PubMed  Google Scholar 

  • Winstead NN, Kelman A (1952) Inoculation techniques for evaluating resistance to Pseudomonas solanacearum. Phytopathology 42:628–634

    Google Scholar 

  • Xu J, Pan ZC, Prior P, Xu JS, Zhang Z, Zhang H, Zhang LQ, He LY, Feng J (2009) Genetic diversity of Ralstonia solanacearum strains from China. Eur J Plant Pathol 125:641–653

    Article  CAS  Google Scholar 

  • Yabuuchi E, Kosako Y, Yano I, Hotta H, Nishiuchi Y (1995) Transfer of two Burkholderia and an Alcaligenes species to Ralstonia gen. nov: proposal for Ralstonia pickettii, Ralstonia solanacearum and Ralstonia eutropha. Microbiol Immunol 39:897–904

    Article  CAS  PubMed  Google Scholar 

  • Zaw MN (2010) Occurrence of bacterial wilt in Kalaw Township and its control measure by effective microorganisms. MS thesis, Yezin Agricultural University, Yezin

    Google Scholar 

Download references

Acknowledgements

We are grateful to the Ministry of Education, Culture, Sports, Science and Technology of Japan, Laboratory of Plant Pathology, Kyushu University of Japan, and Yezin Agricultural University of Myanmar to fulfill this work.

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Authors and Affiliations

  1. Laboratory of Plant Pathology, Faculty of Agriculture, Kyushu University, Fukuoka, 812-8581, Japan

    Htet Wai Wai Kyaw, Kenichi Tsuchiya, Kazuhiro Iiyama & Naruto Furuya

  2. Land-Water Resources and Environment Conservation Section, Institute of Tropical Agriculture, Kyushu University, Fukuoka, 812-8581, Japan

    Masaru Matsumoto

  3. CABI-Europe-UK, Egham, Surrey, TW20 9TY, UK

    Daisuke Kurose

  4. Institute for Agro-Environmental Sciences, National Agricultural Research Organization, Tsukuba, Ibaraki, 305-8604, Japan

    Mitsuo Horita

  5. Department of Plant Pathology, Yezin Agricultural University, Yezin, Naypyidaw, 05282, Myanmar

    Seint San Aye & Myo Zaw

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  1. Htet Wai Wai Kyaw
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  2. Kenichi Tsuchiya
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  9. Naruto Furuya
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Correspondence to Naruto Furuya.

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Kyaw, H.W.W., Tsuchiya, K., Matsumoto, M. et al. Genetic diversity of Ralstonia solanacearum strains causing bacterial wilt of solanaceous crops in Myanmar. J Gen Plant Pathol 83, 216–225 (2017). https://doi.org/10.1007/s10327-017-0720-0

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  • DOI: https://doi.org/10.1007/s10327-017-0720-0

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