Abstract
Mycoviruses associated with hypovirulent phenotypes have been reported for many plant pathogenic fungi. Common techniques to detect mycoviruses depend on the presence of dsRNA elements. These techniques require cultivation of the host fungus, extraction of nucleic acids and purification of dsRNA. These procedures are time-consuming steps and use organic solvents. Here we developed a simple and rapid method detect Magnaporthe oryzae chrysovirus 1-A by direct one-step RT-PCR in samples picked using a sterilized toothpick from fungal colonies growing on plate media. This method could be applied for direct detection of mycoviruses from lesions caused by virus-infected plant pathogenic fungi.
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Acknowledgments
This research was supported in part by a Grant-in-Aid for Linking Mechanism of Research Results to Practical Application from Japan Science and Technology Agency (No. 859100012), by a grant from the New Energy and Industrial Technology Development Organization (No. 08C46503c) to H.M. and by JSPS KAKENHI grants (No. 11J07853 and 24658039) to S.U. and T.A., respectively.
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Urayama, Si., Katoh, Y., Fukuhara, T. et al. Rapid detection of Magnaporthe oryzae chrysovirus 1-A from fungal colonies on agar plates and lesions of rice blast. J Gen Plant Pathol 81, 97–102 (2015). https://doi.org/10.1007/s10327-014-0567-6
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DOI: https://doi.org/10.1007/s10327-014-0567-6