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Differential Proteome Analysis of Hagfish Dental and Somatic Skeletal Muscles

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Abstract

Hagfish, the plesiomorphic sister group of all vertebrates, are deep-sea scavengers. The large musculus (m.) longitudinalis linguae (dental muscle) is a specialized element of the feeding apparatus that facilitates the efficient ingestion of food. In this article, we compare the protein expression in hagfish dental and somatic (the m. parietalis) skeletal muscles via two-dimensional gel electrophoresis and mass spectrometry in order to characterize the former muscle. Of the 500 proteins screened, 24 were identified with significant differential expression between these muscles. The proteins that were overexpressed in the dental muscle compared to the somatic muscle were troponin C (TnC), glycogen phosphorylase, β-enolase, fructose-bisphosphate aldolase A (aldolase A), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). In contrast, myosin light chain 1 (MLC 1) and creatine kinase (CK) were over-expressed in the somatic muscle relative to the dental muscle. These results suggest that these two muscles have different energy sources and contractile properties and provide an initial representative map for comparative studies of muscle-protein expression in low craniates.

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Acknowledgments

We thank Dr. Jen-taie Shiea from Department of Chemistry, National Sun Yat-sen University, for making equipment available to us. This study was partially supported by a grant of the National Science Council of the Republic of China to Hin-Kiu Mok (NSC94-2311-B-110-002). We also thank Dr. Philippe Sebert for reviewing the first draft of the manuscript and two anonymous reviewers for providing helpful comments on a draft of the manuscript.

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Authors and Affiliations

  1. Institute of Marine Biology, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan

    Kuo-Hsun Chiu & Hin-Kiu Mok

  2. Institute of Biomedical Sciences, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan

    Hurng-Wern Huang

  3. Kaohsiung Medical University Joint Research Center, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan

    Hurng-Wern Huang

  4. Asia-Pacific Ocean Research Center, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan

    Hin-Kiu Mok

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  1. Kuo-Hsun Chiu
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Correspondence to Hin-Kiu Mok.

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Chiu, KH., Huang, HW. & Mok, HK. Differential Proteome Analysis of Hagfish Dental and Somatic Skeletal Muscles. Mar Biotechnol 9, 689–700 (2007). https://doi.org/10.1007/s10126-007-9020-6

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