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Effects of IL-23 and IL-27 on osteoblasts and osteoclasts: inhibitory effects on osteoclast differentiation

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Abstract

Interleukin (IL)-23 and IL-27 are IL-6/IL-12 family members that play a role in the regulation of T helper 1 cell differentiation. Cytokines are known to be involved in the bone remodeling process, although the effects of IL-23 and IL-27 have not been clarified. In this study, we examined the possible roles of these cytokines on osteoblast phenotypes and osteoclastogenesis. We found that IL-27 induced signal transducers and activators of transcription 3 activation in osteoblasts. However, neither IL-23 nor IL-27 showed any significant effects on alkaline phosphatase activity, receptor activator of nuclear factor κB ligand (RANKL) expression, mRNA expression such as alkaline phosphatase type I procollagen, or the proliferation of osteoblasts. Osteoclastogenesis from bone marrow cells induced by soluble RANKL was partially inhibited by IL-23 and IL-27 with reduced multinucleated cell numbers, but these interleukins did not affect the proliferation of osteoclast progenitor cells. These results indicate that IL-23 and IL-27 could partly modify cell fusion or the survival of multinucleated osteoclasts. On the other hand, partially purified T cells, which are activated by 2 µg/ml anti-CD3 antibody, completely inhibited osteoclastogenesis by M-CSF/RANKL. On using T cells activated with 0.2 µg/ml anti-CD3 antibody, in which osteoclastogenesis was partially inhibited, the interleukins had additive effects for inhibiting osteoclastogenesis. Although the consequences of phosphorylated signals in osteoblasts have not been identified, IL-23 and IL-27, partly and indirectly through activated T cells, inhibited osteoclastogenesis, indicating that these interleukins may protect against bone destructive autoimmune disorders.

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Authors and Affiliations

  1. Department of Clinical Sciences, Josai International University, 1 Gumyo, Togane, Chiba, 283-8555, Japan

    Sadahiro Kamiya, Takeshi Fukawa, Katsuhiro Ono & Seiki Wada

  2. Department of Bio-analytical Chemistry, Josai International University, Chiba, Japan

    Chika Nakamura

  3. Intractable Immune System Disease Research Center, Tokyo Medical University, Tokyo, Japan

    Toshiyuki Ohwaki & Takayuki Yoshimoto

  4. Department of Patho-Physiology, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba, Japan

    Toshiyuki Ohwaki

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  1. Sadahiro Kamiya
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  2. Chika Nakamura
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  3. Takeshi Fukawa
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  4. Katsuhiro Ono
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  5. Toshiyuki Ohwaki
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  6. Takayuki Yoshimoto
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  7. Seiki Wada
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Correspondence to Sadahiro Kamiya.

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Kamiya, S., Nakamura, C., Fukawa, T. et al. Effects of IL-23 and IL-27 on osteoblasts and osteoclasts: inhibitory effects on osteoclast differentiation. J Bone Miner Metab 25, 277–285 (2007). https://doi.org/10.1007/s00774-007-0766-8

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  • DOI: https://doi.org/10.1007/s00774-007-0766-8

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