Abstract
Two Citrus tristeza virus (CTV) isolates from New Zealand that display distinct phenotypes were isolated, examined and sequenced in full. The first isolate, NZ-M16, is largely asymptomatic and non-transmissible by the aphid vector Toxoptera citricida, while the second, NZ-B18, is highly transmissible and induces very severe symptoms on C. sinensis and C. aurantii. Phylogenetic analysis of the genome sequences showed that both isolates were approximately 90–93% similar to the VT and T318 isolates but possessed only 89% identity to one another. Based on sequence identity, both isolates are VT subtypes, with NZ-M16 being T3-like, while NZ-B18 is a member of a novel subtype with B165 from India.
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Acknowledgments
The authors wish to acknowledge the assistance of N. Tama, University of Auckland, during the graft and aphid transmission experiments. S.J. Harper was supported by a Topachiever Doctoral Scholarship from the New Zealand Tertiary Education Commission.
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Harper, S.J., Dawson, T.E. & Pearson, M.N. Complete genome sequences of two distinct and diverse Citrus tristeza virus isolates from New Zealand. Arch Virol 154, 1505–1510 (2009). https://doi.org/10.1007/s00705-009-0456-z
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DOI: https://doi.org/10.1007/s00705-009-0456-z