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Genome analysis and characterization of a tobacco mosaic virus isolate infecting balsam (Impatiens balsamina)

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Abstract

The complete RNA genomic sequence of a tobacco mosaic virus isolate infecting Impatiens balsamina, designated as TMV-IM, has been determined. The genomic sequence and the predicted gene products of TMV-IM were similar to those of other members of the genus Tobamovirus. The CP ORF of TMV-IM showed sequence identities of 95.0–99.5% with the corresponding ORFs of other TMV strains. Full-length cDNA of TMV-IM was amplified by RT-PCR with a 5′-end primer harboring a T7 promoter sequence and a 3′-end TMV-specific primer. Subsequently, the full-length cDNA was cloned into plasmid vectors. Capped transcripts synthesized from the cDNA clone were highly infectious and caused characteristic symptoms in balsam plants, similar to wild-type TMV-IM and TMV-U1. These results provide definitive evidence for the natural occurrence of TMV in balsam.

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Acknowledgments

We thank Dr. Soo-Chan Lee (Duke University) and our collaborators for helpful suggestions and critical reading. This work was supported by the Korea Research Foundation Grants funded by the Korean Government (MOEHRD, Basic Research Promotion Fund, KRF-2008-314-F00009 and MOEHRD-KRF-2007-359-F00001).

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Authors and Affiliations

  1. Division of Biosciences and Bioinformatics, College of Natural Science, Myong-Ji University, Yongin, 449-728, South Korea

    Seung-Kook Choi

  2. PVGB, Division of Environmental and Life Sciences, Seoul Women’s University, Seoul, 139-774, South Korea

    Ju-Yeon Yoon

  3. Division of Horticultural environment, National Institute of Horticultural and Herbal Science, RDA, Suwon, 440-310, South Korea

    Bong-Nam Chung

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  1. Seung-Kook Choi
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  2. Ju-Yeon Yoon
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  3. Bong-Nam Chung
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Correspondence to Seung-Kook Choi.

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Choi, SK., Yoon, JY. & Chung, BN. Genome analysis and characterization of a tobacco mosaic virus isolate infecting balsam (Impatiens balsamina). Arch Virol 154, 881–885 (2009). https://doi.org/10.1007/s00705-009-0377-x

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  • DOI: https://doi.org/10.1007/s00705-009-0377-x

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