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Pollen morphology of some taxa of Vicia L. subgenus Vicilla (Schur) Rouy (Fabaceae) from Turkey

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Abstract

The pollen morphology of the ten taxa belonging to Cassubicae Radzhi, Cracca Dumort., Ervilia (Link) Koch, Lentopsis Kupicha, Trigonellopsis Rech. f. and Variegatae Radzhi sections of the genus Vicia L. subgenus Vicilla (Schur) Rouy (Fabeae, Fabaceae) in Turkey has been examined by use of light microscopy and scanning electron microscopy. Twelve morphometric characters are analysed with one-way analysis of variance and Tukey’s honestly significant difference test for multiple comparisons. Polar axis and equatorial diameter of pollen grains range from 24.64 to 44.19 and from 15.90 to 30.42 μm, respectively. The pollen grains are prolate–spheroidal to perprolate (P/E = 1.09–2.07), but the prolate shape occurs in the majority of the taxa. The regular pollen grains of all taxa are trizonocolporate, isopolar, and released in monads. Ornamentation of the mesocolpium is psilate–perforate in V. tenuifolia subsp. dalmatica and V. tenuifolia subsp. tenuifolia (sect. Cracca), reticulate–rugulate in V. villosa subsp. villosa (sect. Cracca), and reticulate–perforate in the remaining taxa. The apocolpium and colpus area are reticulate–perforate in V. hirsuta (sect. Cracca), V. ervilia (sect. Ervilia) and V. lunata subsp. grandiflora (sect. Trigonellopsis), and psilate, psilate–slightly perforate or perforate in the other taxa. The results obtained from analyses of pollen grains from the studied taxa of the subgenus Vicilla reveal significant differences in general morphology and some morphometric attributes, and ornamentation types. However, it is concluded that pollen morphology has proved to be uninformative for differentiating the subgenera Vicilla and Vicia.

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Acknowledgments

We would like to thank the Scientific and Technological Research Council of Turkey (TÜBİTAK) and Scientific Research Projects Coordination Unit of Middle East Technical University for financial support, and the curators of following herbaria ANK, E, GAZI, HUB and Muğla Sıtkı Koçman University for allowing us to study their Vicia collections.

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Authors and Affiliations

  1. Department of Biological Sciences, Faculty of Arts and Sciences, Middle East Technical University, 06800, Ankara, Turkey

    Okan Kaan Binzat & Musa Doğan

  2. Department of Biology, Faculty of Arts and Sciences, Uşak University, 64200, Uşak, Turkey

    Ahmet Kahraman

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  1. Okan Kaan Binzat
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  2. Ahmet Kahraman
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  3. Musa Doğan
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Correspondence to Ahmet Kahraman.

Appendix: taxa and source of the material. Species endemic to Turkey is indicated by an asterisk

Appendix: taxa and source of the material. Species endemic to Turkey is indicated by an asterisk

Sect. Cracca Dumort.: V. hirsuta (L.) Gray, İzmir, Bergama to Kozak, Soğanlı, 490 m, 19.05.2011, O. Binzat 322, Sivas, Zara–Şerefiye road, 1,639 m, 22.06.2010, O. Binzat 194; V. tenuifolia Roth subsp. dalmatica (A. Kern.) Greuter, Ankara, 45 km from Ankara to Ayaş, Ayaşbeli, 1,113 m, 28.06.2009, O. Binzat 41, Ankara, Kızılcahamam to Çerkeş, near Güven, 1,165 m. 23.06.2009 O. Binzat 44; V. tenuifolia Roth subsp. tenuifolia, Ankara, Kızılcahamam, Soğuksu National Park, 1,222 m, 23.06.2009, O. Binzat 43, Eskişehir, Türkmendağı Orman İşletme Müdürlüğü, 1,088 m, 27.06.2009 O. Binzat 56, Eskişehir, Mihalıççık to Çalcı village, 1,356 m, 26.06.2009 O. Binzat 49; V. villosa Roth subsp. villosa, Ankara, Esenboğa to Kalecik, near Kalecik, 980 m, 04.07.2010, O. Binzat 247, Eskişehir, Türkmen hill, 1,203 m, 16.06.2010, O. Binzat 151. Sect. Cassubicae Radzhi: V. cassubica L., Eskişehir, Türkmendağı Orman İşletme Müdürlüğü, 1,088 m, 27.06.2009, O. Binzat 55, Eskişehir, Türkmen hill, 1,203 m, 16.06.2010, O. Binzat 153; V. dadianorum Sommier & Levier., Artvin, Hatila valley, Taşlıca village, 1,350 m, 21.07.2011, O. Binzat 380. Sect. Ervilia (Link) Koch: V. ervilia (L.) Willd., Sivas: Hafik road, 1,346 m, 22.06.2010, O. Binzat 193, Ankara, Hacıkadın stream, 980 m. 04.07.2010 O. Binzat 246. Sect. Lentopsis Kupicha: *V. caesarea Boiss. & Balansa, Ankara/Kastamonu, Keltepe, 1,659 m, 24.06.2009, O. Binzat 45, Aksaray, Ihlara, Helvadere village, 1,394 m. 23.05.2010 O. Binzat 101, Nevşehir, Sarıkız, 1,802 m. 18.06.2010 O. Binzat 170. Sect. Trigonellopsis Rech. f.: V. lunata Boiss. subsp. grandiflora (Plitmann) Ponert, Kahramanmaraş, Çimen Mountain, 1,810 m, 25.05.2010 O. Binzat 335; Sect. Variegatae Radzhi: V. canescens Labill. subsp. variegata (Willd.) P. H. Davis, Sivas, 50 km from suşehri to Erzincan Refahiye, 1,600 m, 22.06.2010, O. Binzat 202.

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Binzat, O.K., Kahraman, A. & Doğan, M. Pollen morphology of some taxa of Vicia L. subgenus Vicilla (Schur) Rouy (Fabaceae) from Turkey. Plant Syst Evol 300, 1867–1876 (2014). https://doi.org/10.1007/s00606-014-1013-0

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