Abstract
We determined whether enflurane-induced opisthotonus in ddN mice is mediated by N-methyl-D-aspartate (NMDA) receptor using NMDA receptor antagonists dizocilpine (MK-801) and ketamine. Animals were given intraperitoneal injections of 0.2 ml saline (control), 2.5 or 5.0 mgkg−1 dizocilpine in saline, or 20 or 40 mgkg−1 ketamine in saline 20 min prior to exposure to 2.0% enflurane. Incidence of opisthotonus measured during exposure to enflurane for 20 min was 49% (n=51) in saline (control) group, 6.7 (P < 0.01 vs control, n=30) and 15.0% (P < 0.01, n=40) in 2.5 and 5.0 mgkg−1 dizocilpine group, respectively, and 43.9 (NS, n=41) and 40.0% (NS, n=40) in 20 and 40 mgkg−1 ketamine group, respectively. These results strongly suggest that enflurane-induced opisthotonus is mediated by NMDA receptor. Ketamine failed to suppress significantly due to possibly small dosages. Further, dizocilpine itself produced severe seizures during preenflurane period (30.0 and 40.0% in 2.5 and 5.0 mgkg−1 , respectively), which may be a novel finding.
References
Komatsu H, Ogli K: Opisthotonus during exposure to isoflurane, enflurane, and halothane in mice. Anesthesiology 67: 771–774, 1987
Komatsu H, Ohara T, Nogaya J, et al: The effects of age and anesthetic solubility in anesthetic-induced opisthotonus in mice. J Anesth 5:228–232, 1991
Ruch TC: Brain stem control of posture and orientation in space, The brain and neural function, Physiology and Biophysics, Vol. 1. Edited by Ruch TC, Patton HD. Philadelphia, WB Saunders, 1979, pp 14–52
Watkins JC, Evans RH: Excitatory amino acid neurotransmitters. Ann Rev Pharmacol Toxicol 21:165–204, 1981
Davis J, Evans RH, Jones AW, et al: Differential activation and blockade of excitatory amino acid receptors in mammalian and amphibian CNS. Comp Biochem Physiol 72C:211–224, 1982
McLennan H: Receptors for excitatory amino acids in mammalian central nervous system. Prog Neurobiol 20:251–271, 1984
Croucher MJ, Collins JF, Meldrum BF: Anticonvulsant action of excitatory amino acid antagonists. Science 216:899–901, 1982
Avioli M, Olivier A: Bursting in human epileptogenic neocortex is de pressed by an N-methyl-D-aspartate antagonist. Neurosci Lett 76:249–254, 1987
Gilbert ME: The NMDA-receptor antagonist, MK-801, suppresses limbic kindlings and kindled seizures. Brain Res 463:90–99, 1989
Wong EHF, Kemp JA, Priestley T, et al: The anticonvulsant MK-801 is a potent N-methyl-D-aspartate antagonist. Proc Natl Acad Sci USA 83:7104–7108, 1986
Foster AC, Wong EHF: The novel anticonvulsant MK-801 binds to the activated state of the N-methyl-Daspartate receptor in rat brain. Br J Pharmacol 91:403–409, 1987
Anis NA, Berry SC, Benton NR, et al: The dissociative anesthetics ketamine and phencyclidine, selec- tively reduce excitation of central mammalian neurones by N-methylD-aspartate. Br J Pharmacol 79:565–575, 1983
Yamamura T, Harada K, Okamura A, et al: Is the site of action of ketamine anesthesia the N-methyl-D-aspartate receptor? Anesthesiology 72:704–710, 1990
Wieber J, Gugler R, Hengstmann JH, et al: Pharmacokinetics of ketamine in man. Anaesthetist 24:260–263, 1975
Oswald RL: Injection anesthesia for experimental studies in fish. Comp Biochem Physiol 60C:19–26, 1978
Lodge D, Anis NA: Effects of phencyclidine on excitatory amino acid activation of spinal interneurones in the cat. Eur J Pharmacol 77:203–204, 1982
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Komatsu, H., Nogaya, J., Anabuki, D. et al. The N-Methyl-D-aspartate (NMDA) receptor antagonist dizocilpine (MK-801) suppresses enfturane-induced opisthotonus in mice. J Anesth 7, 520–523 (1993). https://doi.org/10.1007/s0054030070520
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DOI: https://doi.org/10.1007/s0054030070520