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Effect of oxytetracycline on biogas production and active microbial populations during batch anaerobic digestion of cow manure

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Abstract

The aim of this study was to investigate the effect of a common veterinary antibiotic in biogas plants. 20 mg/kg of oxytetracycline was intramuscularly injected into a cow and its concentration in manure, which was sampled daily during the following 20 days, was measured. A total of 20 % of the injected oxytetracycline was detected in manure. Collected manure samples on days 1, 2, 3, 5, 10, 15, and 20 were digested in triplicate serum bottles at 37 °C for 30 days. Control serum bottles produced 255 ± 13 mL biogas, whereas 50–60 % inhibitions were obtained for the serum bottles operated with samples collected for the 5 days after medication. Multivariate statistics used for the evaluation of FISH results showed that Methanomicrobiales were the main methanogenic group responsible for most of the biogas production. Numbers of active Bacteria and Methanomicrobiales were negatively correlated with the presence of oxytetracycline, whereas Methanosarcinales and Methanobacteriales were less affected.

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Acknowledgments

This study was financially supported by the Scientific and Technical Research Council of Turkey (TUBITAK, Project No: 109Y275). The authors are thankful to the Pharmacology and Toxicology Department of Istanbul University, Faculty of Veterinary Medicine for animal medication and manure sampling.

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Author notes

  1. Halil Coban

    Present address: UFZ - Helmholtz Centre for Environmental Research, Department of Environmental Biotechnology, Permoserstr. 15, 04318, Leipzig, Germany

Authors and Affiliations

  1. Institute of Environmental Sciences, Bogazici University, Bebek, 34342, Istanbul, Turkey

    Bahar Ince, Halil Coban, Gokhan Turker & Emine Ertekin

  2. Department of Environmental Engineering, Istanbul Technical University, Maslak, 34469, Istanbul, Turkey

    Orhan Ince

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  1. Bahar Ince
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  2. Halil Coban
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  3. Gokhan Turker
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Correspondence to Emine Ertekin.

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Ince, B., Coban, H., Turker, G. et al. Effect of oxytetracycline on biogas production and active microbial populations during batch anaerobic digestion of cow manure. Bioprocess Biosyst Eng 36, 541–546 (2013). https://doi.org/10.1007/s00449-012-0809-y

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  • DOI: https://doi.org/10.1007/s00449-012-0809-y

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