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Development of a high analytical performance amperometric glucose biosensor based on glucose oxidase immobilized in a composite matrix: layered double hydroxides/chitosan

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Abstract

This paper aimed at showing the interest of the composite material based on layered double hydroxides (LDHs) and chitosan (CHT) as suitable host matrix likely to immobilize enzyme onto electrode surface for amperometric biosensing application. This hybrid material combined the advantages of inorganic LDHs and organic biopolymer, CHT. Glucose oxidase (GOD) immobilized in the composite material maintained its activity well as the usage of glutaraldehyde was avoided. The process parameters for the fabrication of the enzyme electrode and various experimental variables such as pH, applied potential and temperature, were explored for optimum analytical performance of the enzyme electrode. The enzyme electrode provided a linear response to glucose over a concentration range of 1 × 10−6 to 3 × 10−3 M with a high sensitivity of 62.6 mA M−1 cm−2 and a detection limit of 0.1 μM based on the signal-to-noise ratio of 3.

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Acknowledgments

The authors are grateful to the financial supports of National Natural Science Foundation of China (Grant NO. 20505014) and (Grand NO. 20773108), the Key Project of Chinese Ministry of Education (NO. 207041) and Foundation of Jiangsu Provincial Key Program of Physical Chemistry in Yangzhou University.

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Authors and Affiliations

  1. Key Laboratory of Environmental Materials & Environmental Engineering of Jiangsu Province, Yangzhou, 225002, China

    Dan Shan, Wenjuan Yao & Huaiguo Xue

  2. School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, 225002, China

    Qiaofang Shi, En Han, Dan Shan, Wenjuan Yao & Huaiguo Xue

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  1. Qiaofang Shi
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  2. En Han
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  3. Dan Shan
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  4. Wenjuan Yao
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  5. Huaiguo Xue
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Correspondence to Dan Shan or Huaiguo Xue.

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Shi, Q., Han, E., Shan, D. et al. Development of a high analytical performance amperometric glucose biosensor based on glucose oxidase immobilized in a composite matrix: layered double hydroxides/chitosan. Bioprocess Biosyst Eng 31, 519–526 (2008). https://doi.org/10.1007/s00449-007-0190-4

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  • DOI: https://doi.org/10.1007/s00449-007-0190-4

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