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Coinfection with Clonorchis sinensis modulates murine host response against Trichinella spiralis infection

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Abstract

Concomitant infections of different species of parasites are common in the field. Infection with one parasite species likely triggers host responses that may influence the subsequent infection of another species and alter disease outcomes. So far, the majority of studies have focused on single species parasite infection, and the mechanisms of protection induced by the first parasite infection against the secondary infection remain poorly defined. In this study, we assess the impact of trematode Clonorchis sinensis infection on the course of another tissue nematode Trichinella spiralis challenge. We observed that mice with preexisting C. sinensis infection had lower worm burden of intestinal T. spiralis than those infected with T. spiralis alone; mice with preexisting C. sinensis also had severe enteric histopathological changes and higher counts of intestinal Paneth cells in responses to T. spiralis challenge. The mRNA levels of interleukin (IL)-4, IL-10, IL-13, and tumor necrosis factor (TNF)-α from the small intestine and spleen of the different groups were analyzed using quantitative real-time polymerase chain reaction. Compared with that in mice infected with T. spiralis alone, the mRNA expression of IL-13 was significantly increased in the small intestine tissues and IL-4, IL-13, and TNF-α were significantly increased in the spleen tissues in the dually infected mice. Our findings suggest that a “preexisting” trematode infection of C. sinensis is a factor which contributes to reducing the establishment of T. spiralis adult worms in the small intestine.

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Acknowledgments

We thank Dr. Xin-zhuan Su (LMVR/NIAID/NIH, USA) for his valuable advice and critical reading of the manuscript. This work was supported, in part, by grants from the National Basic Research Program of China (973 Program) (No. 2010CB530000) and the Natural Science Foundation of China to F. Lu (nos. 81071387 and 81271854).

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Authors and Affiliations

  1. Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, Guangdong, China

    Ying Chen, Bo Huang, Xinbing Yu & Fangli Lu

  2. Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of Education, Guangzhou, 510080, Guangdong, China

    Ying Chen, Bo Huang, Xinbing Yu & Fangli Lu

  3. School of Medicine, Jinan University, Guangzhou, 510632, China

    Shiguang Huang

  4. Department of Parasitology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Yonglong Li

  5. Department of Pathogen Biology, School of Medicine, Jianghan University, Wuhan, 430056, China

    Wenjian Song

  6. Department of Pathogen Biology and Immunology, College of Medicine, Shihezi University, Shihezi, 832002, China

    Yongxiang Li

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  1. Ying Chen
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  2. Bo Huang
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Correspondence to Shiguang Huang or Fangli Lu.

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Chen, Y., Huang, B., Huang, S. et al. Coinfection with Clonorchis sinensis modulates murine host response against Trichinella spiralis infection. Parasitol Res 112, 3167–3179 (2013). https://doi.org/10.1007/s00436-013-3493-1

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  • DOI: https://doi.org/10.1007/s00436-013-3493-1

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