Abstract
This study was undertaken to identify genes involved in the growth and development of Spirometra erinacei larvae, an intestinal tapeworm of cats and dogs, within the final host. The differential protein expression at three different stages of S. erinacei, the plerocercoid larvae, 8-day-old juveniles, and adults, was compared using two-dimensional electrophoresis. Specifically or highly expressed proteins in juvenile worms were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MS)/MS. The proteome map of larvae showed fewer protein spots than juveniles or adults, whereas juveniles or adults revealed a similar protein expression profile. Eight juvenile-specific and five upregulated proteins of juveniles were identified and matched to proteins of known biological functions. These were grouped into several categories of functionally related proteins: DNA/RNA metabolism, cell trafficking, cytoskeleton, protein processing and degradation, energy metabolism, and oxidative stress. Our results give an overview of the growth and development mechanisms of cestodes within the final host and extend our understanding of parasite biology in the host–parasite relationship.
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Adroher FJ, Osuna A, Lupianez JA (1988) Differential energetic metabolism during Trypanosoma cruzi differentiation. I. Citrate synthase, NADP-isocitrate dehydrogenase, and succinate dehydrogenase. Arch Biochem Biophys 267:252–261
Bente M, Harder S, Wiesgigl M, Heukeshoven J, Gelhaus C, Krause E, Clos J, Bruchhaus I (2003) Developmentally induced changes of the proteome in the protozoan parasite Leishmania donovani. Proteomics 3:1811–1829
Browner MF, Taroni F, Sztul E, Rosenberg LE (1989) Sequence analysis, biogenesis, and mitochondrial import of the alpha-subunit of rat liver propionyl-CoA carboxylase. J Biol Chem 264:12680–12685
Chemale G, van Rossum AJ, Jefferies JR, Barrett J, Brophy PM, Ferreira HB, Zaha A (2003) Proteomic analysis of the larval stage of the parasite Echinococcus granulosus: causative agent of cystic hydatid disease. Proteomics 3:1633–1636
Chung YB, Yang HJ (2008) Partial purification and characterization of a cysteine protease inhibitor from the plerocercoid of Spirometra erinacei. Korean J Parasitol 46:183–186
Damer CK, Bayeva M, Kim PS, Ho LK, Eberhardt ES, Socec CI, Lee JS, Bruce EA, Goldman-Yassen AE, Naliboff LC (2007) Copine A is required for cytokinesis, contractile vacuole function, and development in Dictyostelium. Eukaryot Cell 6:430–442
Ding X, Jin Y, Wu Y, Wu H, Xiong L, Song X, Liu S, Fan W, Fan M (2008) Localization and cellular distribution of CPNE5 in embryonic mouse brain. Brain Res 1224:20–28
El Fakhry Y, Ouellette M, Papadopoulou B (2002) A proteomic approach to identify developmentally regulated proteins in Leishmania infantum. Proteomics 2:1007–1017
Fielenbach N, Antebi A (2008) C. elegans dauer formation and the molecular basis of plasticity. Genes Dev 22:2149–2165
Fujita N, Jaye DL, Kajita M, Geigerman C, Moreno CS, Wade PA (2003) MTA3, a Mi-2/NuRD complex subunit, regulates an invasive growth pathway in breast cancer. Cell 113:207–219
Gothel SF, Marahiel MA (1999) Peptidyl-prolyl cis–trans isomerases, a superfamily of ubiquitous folding catalysts. Cell Mol Life Sci 55:423–436
Ji Q, Huang CH, Peng J, Hashmi S, Ye T, Chen Y (2007) Characterization of STIP, a multi-domain nuclear protein, highly conserved in metazoans, and essential for embryogenesis in Caenorhabditis elegans. Exp Cell Res 313:1460–1472
Jolly ER, Chin CS, Miller S, Bahgat MM, Lim KC, DeRisi J, McKerrow JH (2007) Gene expression patterns during adaptation of a helminth parasite to different environmental niches. Genome Biol 8:R65
Kubota H, Hynes G, Willison K (1995) The chaperonin containing t-complex polypeptide 1 (TCP-1). Multisubunit machinery assisting in protein folding and assembly in the eukaryotic cytosol. Eur J Biochem 230:3–16
Laity JH, Lee BM, Wright PE (2001) Zinc finger proteins: new insights into structural and functional diversity. Curr Opin Struct Biol 11:39–46
Lee SH, We JS, Sohn WM, Hong ST, Chai JY (1990) Experimental life history of Spirometra erinacei. Korean J Parasitol 28:161–173
Markell EK, Voge M, John DT (1986) Parasites, parasitism and host relations. In: Markell EK, Voge M, John DT (eds) Medical parasitology, 6th edn. W.B. Saunders Company: Philadelphia (pp 5-19)
Massey HC Jr, Nishi M, Chaudhary K, Pakpour N, Lok JB (2003) Structure and developmental expression of Strongyloides stercoralis fktf-1, a proposed ortholog of daf-16 in Caenorhabditis elegans. Int J Parasitol 33:1537–1544
Millevoi S, Trombitas K, Kolmerer B, Kostin S, Schaper J, Pelin K, Granzier H, Labeit S (1998) Characterization of nebulette and nebulin and emerging concepts of their roles for vertebrate Z-discs. J Mol Biol 282:111–123
Moncman CL, Wang K (1995) Nebulette: a 107 kD nebulin-like protein in cardiac muscle. Cell Motil Cytoskeleton 32:205–225
Nishisaka M, Yokoyama N, Xuan X, Inoue N, Nagasawa H, Fujisaki K, Mikami T, Igarashi I (2001) Characterisation of the gene encoding a protective antigen from Babesia microti identified it as eta subunit of chaperonin containing T-complex protein 1. Int J Parasitol 31:1673–1679
Park YK, Park JH, Guk SM, Shin EH, Chai JY (2005) A new method for concentration of proteins in the calcareous corpuscles separated from the spargana of Spirometra erinacei. Korean J Parasitol 43:119–122
Redowicz MJ (2007) Unconventional myosins in muscle. Eur J Cell Biol 86:549–558
Suraweera A, Becherel OJ, Chen P, Rundle N, Woods R, Nakamura J, Gatei M, Criscuolo C, Filla A, Chessa L, Fusser M, Epe B, Gueven N, Lavin MF (2007) Senataxin, defective in ataxia oculomotor apraxia type 2, is involved in the defense against oxidative DNA damage. J Cell Biol 177:969–979
Tkachuck RD, Saz HJ, Weinstein PP, Finnegan K, Mueller JF (1977) The presence and possible function of methylmalonyl CoA mutase and propionyl CoA carboxylase in Spirometra mansonoides. J Parasitol 63:769–774
Ursic D, Himmel KL, Gurley KA, Webb F, Culbertson MR (1997) The yeast SEN1 gene is required for the processing of diverse RNA classes. Nucleic Acids Res 25:4778–4785
Wang Y, Ma Y, Lu B, Xu E, Huang Q, Lai M (2007) Differential expression of mimecan and thioredoxin domain-containing protein 5 in colorectal adenoma and cancer: a proteomic study. Exp Biol Med (Maywood) 232:1152–1159
Weber JM, Ruzindana-Umunyana A, Imbeault L, Sircar S (2003) Inhibition of adenovirus infection and adenain by green tea catechins. Antiviral Res 58:167–173
Wen X, Lei YP, Zhou YL, Okamoto CT, Snead ML, Paine ML (2005) Structural organization and cellular localization of tuftelin-interacting protein 11 (TFIP11). Cell Mol Life Sci 62:1038–1046
Westermann S, Weber K (2003) Post-translational modifications regulate microtubule function. Nat Rev Mol Cell Biol 4:938–947
Acknowledgment
This work was supported by an Anti-Communicable Diseases Control Program of the National Institute of Health (NIH 348-6111-215), Ministry of Health and Welfare, Republic of Korea, 2005–2007.
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Kim, JH., Kim, Y.J., Sohn, WM. et al. Differential protein expression in Spirometra erinacei according to its development in its final host. Parasitol Res 105, 1549–1556 (2009). https://doi.org/10.1007/s00436-009-1585-8
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DOI: https://doi.org/10.1007/s00436-009-1585-8