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Discovery of Paragonimus proliferus in Northern Vietnam and their molecular phylogenetic status among genus Paragonimus

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Abstract

During an epidemiological survey for Paragonimus and paragonimosis in northern Vietnam, we found extremely large excysted metacercariae (2.50 ± 0.14 mm in length and 0.72 ± 0.08 mm in width; mean ± standard deviation of 20 samples) in mountainous crabs, Potamiscus mieni. Adult worms were successfully obtained by intraperitoneal injection with those large excysted metacercariae in a cat. Morphological and morphometric data of those large excysted metacercariae and the adult worms derived from them are identical to those of Paragonimus proliferus found in Yunnan province, China. However, when second internal transcribed spacer region and cytochrome oxidase subunit 1 gene sequences of those metacercariae and adult worms were compared with those of known Paragonimus spp. deposited in the GenBank, they were almost completely identical to those of Paragonimus hokuoensis metacercariae in China, of which adult worms have never been reported. This is the first record of P. proliferus in Vietnam and the first record from outside of China. Phylogenetic relationship between P. proliferus and P. hokuoensis is discussed.

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Acknowledgements

The authors are grateful to Prof. Zhou Ben-jang, Department of Parasitology, Kunming Medical College, for confirmation of morphological identification of P. proliferus. Special thanks go to Dr. David Blair, James Cook University, for providing us with valuable information on Paragonimus spp., in China. One of the authors, Pham Ngoc Doanh, received financial support from the Japanese Society for Promotion of Science (JSPS) Ronpaku Fellowship No. NCST-10430 for a Ph.D. degree.

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Correspondence to Yoichiro Horii.

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Doanh, P.N., Shinohara, A., Horii, Y. et al. Discovery of Paragonimus proliferus in Northern Vietnam and their molecular phylogenetic status among genus Paragonimus . Parasitol Res 102, 677–683 (2008). https://doi.org/10.1007/s00436-007-0811-5

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  • DOI: https://doi.org/10.1007/s00436-007-0811-5

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