Abstract
The complete internal transcribed spacer 1 (ITS1), 5.8S ribosomal DNA, and ITS2 region of the ribosomal DNA from 60 specimens belonging to two closely related bucephalid digeneans (Dollfustrema vaneyi and Dollfustrema hefeiensis) from different localities, hosts, and microhabitat sites were cloned to examine the level of sequence variation and the taxonomic levels to show utility in species identification and phylogeny estimation. Our data show that these molecular markers can help to discriminate the two species, which are morphologically very close and difficult to separate by classical methods. We found 21 haplotypes defined by 44 polymorphic positions in 38 individuals of D. vaneyi, and 16 haplotypes defined by 43 polymorphic positions in 22 individuals of D. hefeiensis. There is no shared haplotypes between the two species. Haplotype rather than nucleotide diversity is similar between the two species. Phylogenetic analyses reveal two robustly supported clades, one corresponding to D. vaneyi and the other corresponding to D. hefeiensis. However, the population structures between the two species seem to be incongruent and show no geographic and host-specific structure among them, further indicating that the two species may have had a more complex evolutionary history than expected.
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Acknowledgments
The first author thanks Dr. Q. Gao for assistance in collecting specimens. We are grateful to Dr. X. Guo (Chengdu Institute of Biology, Chinese Academy of Sciences) for his kind help on the Bayesian hypothesis testing and helpful comments on multiple drafts of this manuscript. We also gracefully acknowledge the two anonymous reviewers and the editor whose critical review and insightful suggestions have helped to improve the clarity and focus of the manuscript. This study was supported by grants (project no. 30130150) from the National Natural Science Foundation of China and a grant [project no. (2005) 192] from the Chinese Academy of Sciences.
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Chen, D., Wang, G., Yao, W. et al. Utility of ITS1–5.8S–ITS2 sequences for species discrimination and phylogenetic inference of two closely related bucephalid digeneans (Digenea: Bucephalidae): Dollfustrema vaneyi and Dollfustrema hefeiensis . Parasitol Res 101, 791–800 (2007). https://doi.org/10.1007/s00436-007-0549-0
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DOI: https://doi.org/10.1007/s00436-007-0549-0