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Molecular identification of Trichinella spiralis and Trichinella britovi by diagnostic multiprimer large mitochondrial rRNA amplification

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Abstract

Trichinella parasites with different epidemiological features still occur in Europe and four species of genus Trichinella have been identified: T. spiralis, T. britovi , T. nativa and T. pseudospiralis. Until now, two of them, T. spiralis and T. britovi, have been identified in Poland. In our studies we selected sequence coding for large mitochondrial rRNA (mt LrDNA) as a genetic marker and developed a sensitive LrDNA multiprimer PCR assay allowing for rapid identification of T. spiralis and T. britovi, parasites present in wild and domestic animals in Poland.

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References

  • Bandi C, La Rosa G, Bardin MG, Damiani G, Comincini L, Tasciotti L, Pozio E (1995) Random amplified polymorphic DNA fingerprints of the eight taxa of Trichinella and their comparison with allozyme analysis. Parasitology 110:401–407

    CAS  PubMed  Google Scholar 

  • Cabaj W, Przyjałkowski Z (1987) Biological characteristics of Trichinella spiralis and T. pseudospiralis infections in mice. Acta Parasitol Pol 32:195–204

    Google Scholar 

  • Cabaj W, Pozio E, Moskwa B, Malczewski A (2000) Trichinella britovi and T. spiralis in red foxes ( Vulpes vulpes) in Poland. Acta Parasitol 45:340–344

    Google Scholar 

  • Dupouy-Camet J, Robert F, Guillou JP, Vallet C, Perret C, Soule C (1994) Identyfication of Trichinella isolates with random amplified polymorphic DNA markers. Parasitol Res 80:358–60

    CAS  PubMed  Google Scholar 

  • Gasser RB, Zhu X, Monti JR, Dou L, Cai X, Pozio E (1998) PCR-SSCP of RDNA for the identification of Trichinella isolates from mainland China. Mol Cell Probes 12:27–34

    Article  CAS  PubMed  Google Scholar 

  • Nagano I, Wu Z, Matsuo A, Pozio E, Takahashi Y (1999) Identification of Trichinella isolates by polymerase chain reaction-restriction fragment length polymorphism of the mitochondrial cytochrome c-oxidase subunit I gene. Int J Parasitol 29:1113–1120

    Article  CAS  PubMed  Google Scholar 

  • Nowosad P, Pozio E (1998) First report of Trichinella britovi in wildlife from Poland. Acta Parasitol 43:236–237

    Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) CLUSTAL X Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tool. Nucleic Acids Res 24:4876–4882

    Article  Google Scholar 

  • Wu Z, Nagano I, Pozio E, Takahashi Y (1999) Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for the identification of Trichinella isolates. Parasitology 118:211–218

    Article  CAS  PubMed  Google Scholar 

  • Zarlenga DS, Chute MB, Martin A, Kapel CMO (1999) A multiplex PCR for unequivocal differentiation of encapsulated and non-encapsulated genotypes of Trichinella. Int J Parasitol 29:1859–1867

    Article  CAS  PubMed  Google Scholar 

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Acknowledgement

This work was supported by research grant no. 6 P04C 02218 from the State Committee for Scientific Research, Warsaw.

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Authors and Affiliations

  1. Department of Genetics, University of Warsaw, Pawinskiego 5A, 02–106, Warsaw, Poland

    P. Borsuk

  2. Institute of Biochemistry and Biophysics of the Polish Academy of Science, Pawinskiego 5A, 02–106, Warsaw, Poland

    P. Borsuk

  3. Witold Stefanski Institute of Parasitology of the Polish Academy of Science, Twarda 51/55, 00–818, Warsaw, Poland

    B. Moskwa, K. Pastusiak & W. Cabaj

Authors
  1. P. Borsuk
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  2. B. Moskwa
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  3. K. Pastusiak
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  4. W. Cabaj
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Correspondence to P. Borsuk.

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Borsuk, P., Moskwa, B., Pastusiak, K. et al. Molecular identification of Trichinella spiralis and Trichinella britovi by diagnostic multiprimer large mitochondrial rRNA amplification. Parasitol Res 91, 374–377 (2003). https://doi.org/10.1007/s00436-003-0971-x

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  • DOI: https://doi.org/10.1007/s00436-003-0971-x

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