Abstract
There are more than 100 candidate genes of asthma located on 23 human chromosomes. Interleukin-4 (IL-4), located on chromosome 5q31, and ADAM33, located on chromosome 20p13, and some single nucleotide polymorphisms (SNPs) of these genes have been shown to be associated with asthma and its manifestations in different populations. The most prominent SNPs of IL-4 and ADAM33 are 589C>T and 400A>G, respectively. There are also controversial reports on the association of these SNPs with asthma. In the present study, we analyzed these two SNPs in 100 patients with asthma and 50 controls through PCR amplification and restriction digestion to evaluate association of these two SNPs with asthma. The nonsignificant differences were observed for the IL-4 promoter polymorphism C589T and the ADAM33 T1 polymorphism between asthmatic patients and controls (P = 0.638 and 0.943, respectively). Our data revealed that there is no association of these SNPs with asthma indicating that other SNPs of these genes or other genes might be involved in the manifestation of asthma.
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References
Palmer LJ, Cookson WOCM (2000) Genomic approaches to understanding asthma. Genome Res 10:1280–1287
Aggarwal AN, Chaudhry K, Chhabra SK, D’Souza GA, Gupta D, Jindal SK, Katiyar SK, Kumar R, Shah B, Vijayan VK (2006) Prevalence and risk factors for bronchial asthma in Indian adults: a multicentre study. Indian J Chest Dis Allied Sci 48:13–22
Paramesh H (2002) Epidemiology of asthma in India. Indian J Pediatr 69(4):309–312
ISAAC Steering Committee (1998) Worldwide variations in the prevalence of asthma symptoms: the International Study of Asthma and Allergies in Childhood (ISAAC). Eur Respir J 12:315–335
Lee YC, Cheon KT, Lee HB, Kim W, Rhee YK, Kim DS (2000) Gene polymorphisms of endothelial nitric oxide synthase and angiotensin-converting enzyme in patient with asthma. Allergy 55:959–963
McFadden JER (2005) Asthma. In: Fauci AS, Braunwald E, Kasper DL, Hauser SL, Longo DL, Jameson JL (eds) Harrison’s principles of internal medicine. McGraw-Hill, New York, pp 1508–1516
Los H, Koppelman GH, Postma DS (1999) The importance of genetic influences in asthma. Eur Respir J 14:1210–1227
Palmer LJ, Cookson WOCM (2001) Using single nucleotide polymorphisms as a means to understanding the pathophysiology of asthma. Respir Res 2:102–112
Chiang CH, Tang YC, Lin MW, Chung MT (2007) Association between the IL-4 promoter polymorphisms and asthma or severity of hyperresponsiveness in Taiwanese. Respirology 12:42–48
Marsh DG, Neely JD, Breazeale DR, Ghosh B, Freidhoff LR, Ehrich-Kautzky E, Schou C, Krishnaswamy G, Beaty TH (1994) Linkage analysis of IL4 and other chromosome 5q31.1 markers and total serum immunoglobulin E concentrations. Science 264:1152–1156
van Eerdewegh PV, Little RD, Dupuis J, Del Mastro RG, Falls K, Simon J (2002) Association of the ADAM33 gene with asthma and bronchial hyperresponsivenes. Nature 418:426–430
Postma DS, Koppelman GH (2009) Genetics of asthma: where are we and where do we go? Proc Am Thorac Soc 6:283–287
Howard TD, Postma DS, Jongepier H, Moore WC, Koppelman GH (2003) Association of a disintegrin and metalloprotease 33 (ADAM33) gene with asthma in ethnically diverse populations. J Allergy Clin Immunol 112(4):717–722
Su D, Zhang X, Sui H, Lu F, Jin L, Zhang J (2008) Association of ADAM33 gene polymorphisms with adult allergic asthma and rhinitis in a Chinese Han population. BMC Med Genet 9:82–87
GINA executive committee (2008) Global strategy for asthma management 2008. In: O’Byme P (ed) Global initiative for asthma. McMaster University, Montreal, pp 16–96
Saviour P, Kumar S, Kiran U, Ravuri RR, Rao VR, Ramachandra NB (2008) Allelic variants of DYX1C1 are not associated with dyslexia in India. Indian J Hum Genet 14(3):99–102
Manolio TA, Collins FS, Cox NJ, Goldstein DB, Hindorff LA, Hunter DJ (2009) Finding the missing heritability of complex diseases. Nature 461:747–753
Noguchi E, Shibasaki M, Arinami T, Takeda K, Yokouchi Y, Kawashima T (1998) Association of asthma and the interleukin-4 promoter gene in Japanese. Clin Exp Allergy 28(4):449–453
Kabesch M, Tzotcheva I, Carr D, Holfer C, Weiland SK, Fritzsch C (2003) A complete screening of the IL4 gene: novel polymorphisms and their association with asthma and IgE in childhood. J Allergy Clin Immunol 112:893–898
Walley AJ, Cookson WOCM (1996) Investigation of an interleukin-4 promoter polymorphism for associations with asthma and atopy. J Med Genet 33:689–692
Hijazi Z, Haider MZ (2000) Interleukin-4 gene promoter poly morphism [C590T] and asthma in Kuwaiti Arabs. Int Arch Allergy Immunol 122:190–194
Cui T, Wu J, Pan S, Xie J (2003) Polymorphisms in the IL-4 and IL-4R [alpha] genes and allergic asthma. Clin Chem Lab Med 41:888–892
Nagarkatti R, Kumar R, Sharma SK, Ghosh B (2004) Association of IL4 gene polymorphisms with asthma in North Indians. Int Arch Allergy Immunol 134:206–212
de Faria IC, de Faria EJ, Toro AA, Ribeiro JD, Bertuzzo CS (2008) Association of TGF-1, CD14, IL-4, IL-4R and ADAM33 gene polymorphisms with asthma severity in children and adolescents. J Pediatr (Rio J) 84(3):203–210
Holgate ST, Davies DE, Rorke S, Cakebread J, Murphy G, Powell RM, Holloway JW (2004) ADAM33 and its association with airway remodeling and hyperresponsiveness in asthma. Clin Rev Allergy Immunol 27:23–34
van Diemen CC, Postma DS, Vonk JM, Bruinenberg M, Schouten JP, Boezen HM (2005) A disintegrin and metalloprotease 33 polymorphisms and lung function decline in the general population. Am J Respir Crit Care Med 172:329–333
Simpson A, Maniatis N, Jury F, Cakebread JA, Lowe LA, Holgate ST (2005) Polymorphisms in a disintegrin and metalloprotease 33 (ADAM33) predict impaired early-life lung function. Am J Respir Crit Care Med 172:55–60
Kedda M, Duffy DL, Bradley B, O’Hehir RE, Thompson PJ (2006) ADAM33 haplotypes are associated with asthma in a large Australian population. Eur J Hum Genet 14:1027–1036
Noguchi E, Ohtsuki Y, Tokunaga K, Yamaoka-Sageshima M, Ichikawa K, Aoki T (2006) ADAM33 polymorphisms are associated with asthma susceptibility in a Japanese population. Clin Exp Allergy 36:602–608
Hirota T, Hasegawa K, Obara K, Matsuda A, Akahoshi M, Nakashima K (2006) Association between ADAM33 polymorphisms and adult asthma in the Japanese population. Clin Exp Allergy 36:884–891
Sakagami T, Jinnai N, Nakajima T, Sekigawa T, Hasegawa T, Suzuki E (2007) ADAM33 polymorphisms are associated with aspirin-intolerant asthma in the Japanese population. J Hum Genet 52:66–72
Lind DL, Choudhry S, Ung N, Ziv E, Avila PC, Salari K (2003) ADAM33 is not associated with asthma in Puerto Rican or Mexican populations. Am J Respir Crit Care Med 168:1312–1316
Lee JH, Park HS, Park SW, Jang AS, Uh ST, Rhim T (2004) ADAM33 polymorphism: association with bronchial hyper-responsiveness in Korean asthmatics. Clin Exp Allergy 34:860–865
Raby BA, Silverman EK, Kwiatkowski DJ, Lange C, Lazarus R, Weiss ST (2004) ADAM33 polymorphisms and phenotype associations in childhood asthma. J Allergy Clin Immunol 113:1071–1078
Bijanzadeh M, Ramachandra NB, Mahesh PA, Savitha MR, Vijayakumar GS, Kumar P, Manjunath BS, Jayaraj BS (2009) Soluble intercellular adhesion molecule-1 and E-selectin in patients with asthma exacerbation. Lung 187:315–320
Werner M, Herbon N, Gohlke H, Altmüller J, Knapp M, Heinrich J, Wjst M (2004) Asthma is associated with single-nucleotide polymorphisms in ADAM33. Clin Exper Allerg 34:26–31
Scirica CV, Celedon JC (2007) Genetics of asthma, potential implications for reducing asthma disparities. Chest 132:770S–781S
Acknowledgment
The authors thank the doctors, patients, and patients’ families in Cheluvamba Hospital and JSS Hospital and in the Allergy, Asthma and Chest Center of Mysore; Dr. S. Pushpa, Principal, Mysore Medical College & Research Institute; Dr. Rajendra Kumar, Cheluvamba Hospital, Mysore Medical College & Research Institute; Dr. S.K. Chaya and Dr. A.K. Prabhakar, JSS Hospital, Mysore; Mr. M. Kareem Khan and Mrs. Sumithra, and also all the members of N. B. Ramachandra’s research group for their valuable help and suggestions. Mahdi Bijanzadeh is grateful to the University of Mysore, Chairman, DOS in Zoology, core group of the Unit on Evolution and Genetics, and Ahwaz University of Medical Sciences, Iran, for providing facilities to perform this work.
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Bijanzadeh, M., Ramachandra, N.B., Mahesh, P.A. et al. Association of IL-4 and ADAM33 Gene Polymorphisms with Asthma in an Indian Population. Lung 188, 415–422 (2010). https://doi.org/10.1007/s00408-010-9247-2
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DOI: https://doi.org/10.1007/s00408-010-9247-2