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Laryngeal carcinoma lymph node metastasis and disease-free survival correlate with MASPIN nuclear expression but not with EGFR expression: a series of 108 cases

  • Laryngology
  • Published:
European Archives of Oto-Rhino-Laryngology Aims and scope Submit manuscript

Abstract

Despite advances in laryngeal squamous cell carcinoma (LSCC) treatment, patient survival has not improved in the last two decades. Novel, more effective strategies should be based on receptor-mediated LSCC-targeted therapy. The epidermal growth factor receptor (EGFR) is the most widely studied molecular target. MASPIN multifaceted anti-tumor effects have been rarely evaluated in LSCC. The aims of this study were to investigate EGFR and MASPIN expression and the role of sub-cellular MASPIN localization in LSSC. MASPIN and EGFR expression and the sub-cellular localization of MASPIN were assessed using a computerized image analysis system in 108 consecutive cases of operable LSCC. The rates of occurrence of lymph node metastases and recurrent disease were lower in patients with a nuclear pattern of MASPIN expression (p = 0.004, p = 0.0028). As expected, the loco-regional recurrence rate was lower in N0 patients (p = 0.009), but the disease recurrence rate was even lower in N0 patients with a nuclear localization of MASPIN (p = 0.020). Disease-free survival was longer in cases of LSCC with a nuclear MASPIN pattern (p = 0.003). The intensity of EGFR reactivity and the EGFR area fraction were unrelated to the clinico-pathological and prognostic parameters in LSCC. A nuclear MASPIN pattern is a promising prognostic indicator in LSCC, but further evidence is needed before elective neck dissection can be considered for cN0 LSCCs with a non-nuclear MASPIN pattern. Although a better understanding of the mechanisms behind sub-cellular MASPIN localization is mandatory, re-activating nuclear MASPIN in association with specific chemotherapeutic agents may be an important goal in the treatment of LSCC.

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Acknowledgments

The authors would like to thank Mrs Vincenza Guzzardo, Department of Medical/Diagnostic Sciences and Special Therapies, University of Padova, for her excellent technical assistance and Mrs Frances Coburn for checking the English version of this paper. This study has been supported in part by grant nr 60A07-4404/09 (G.M.) from University of Padova, Italy.

Conflict of interest statement

The authors declare no financial and personal relationships with other people or organisations that could inappropriately influence (bias) their work.

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Authors and Affiliations

  1. Otolaryngology Section, Department of Medical and Surgical Specialties, University of Padova, Via Giustiniani 2, 35128, Padua, Italy

    Gino Marioni, Alberto Staffieri, Giancarlo Ottaviano, Daria Accordi & Cosimo de Filippis

  2. Otolaryngology Unit, Vittorio Veneto Hospital, Vittorio Veneto, Italy

    Andy Bertolin

  3. Anatomic Pathology Section, Department of Medical/Diagnostic Sciences and Special Therapies, University of Padova, Padua, Italy

    Luciano Giacomelli, Emiliano D’Alessandro & Stella Blandamura

  4. Radiology Section, Department of Medical/Diagnostic Sciences and Special Therapies, University of Padova, Padua, Italy

    Roberto Stramare

Authors
  1. Gino Marioni
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  2. Alberto Staffieri
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  3. Andy Bertolin
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  4. Luciano Giacomelli
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  5. Emiliano D’Alessandro
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  6. Giancarlo Ottaviano
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  7. Daria Accordi
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  8. Roberto Stramare
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  9. Cosimo de Filippis
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  10. Stella Blandamura
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Corresponding author

Correspondence to Gino Marioni.

Additional information

A preliminary version of this study won the first prize for the Gradenigo–Ferreri–Bilancioni Annual National Award at the Italian Otolaryngological Society (SIO) Meeting, Rimini, 13th–16th May 2009.

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Marioni, G., Staffieri, A., Bertolin, A. et al. Laryngeal carcinoma lymph node metastasis and disease-free survival correlate with MASPIN nuclear expression but not with EGFR expression: a series of 108 cases. Eur Arch Otorhinolaryngol 267, 1103–1110 (2010). https://doi.org/10.1007/s00405-009-1186-2

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  • DOI: https://doi.org/10.1007/s00405-009-1186-2

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