Abstract
Objective
This study aimed to investigate the effect exerted by teriparatide on the repair of femoral metaphyseal defect in ovariectomized rats.
Method
Female Sprague–Dawley rats were ovariectomized and after 3 months a critically sized defect of 3 mm in diameter—a through-hole bone defect—was drilled into each distal femur of the ovariectomized rats. The rats were injected with teriparatide (30 μg/kg) parathyroid hormone (PTH) in the peritoneum three times per week. After 4 and 8 weeks the animals were killed and the blood and bilateral femora were harvested for biochemical analysis, histopathological observation, and micro-computed tomography (CT) examination.
Results
The PTH group and control group were compared 4 and 8 weeks after surgery. PTH increased bone formation in the defect area. Moreover, PTH showed the strongest effects on bone volume per total volume, trabecular number, trabecular thickness, trabecular separation, and total fluorescence-marked new bone area. Additionally, the PTH treatment group showed inhibited serum concentrations of C-terminal telopeptide of type I collagen and enhanced expression of calcium, phosphorus, and bone alkaline phosphatase.
Conclusion
Our findings suggest a positive effect of PTH on defect healing in ovariectomized rats.
Zusammenfassung
Ziel
Ziel war die Untersuchung der Wirkung von Teriparatid auf die Heilung bei einem Femurmetaphysendefekt ovarektomierter Ratten.
Methode
Bei seit 3 Monaten ovarektomierten weiblichen Sprague-Dawley(SD)-Ratten wurde ein Defekt kritischer Größe von 3 mm Durchmesser mit einer Durchgangsbohrung in jedem distalen Femur der ovarektomierten Ratten erzeugt. Den Ratten wurde 3-mal pro Woche PTH-Teriparatid (30 μg/kg) in das Peritoneum injiziert. Nach 4 bzw. 8 Wochen wurden die Tiere getötet, Blut und die Femora beidseits wurden für biochemische und histopathologische Untersuchungen sowie für eine Mikro-CT-Untersuchung entnommen.
Ergebnisse
Die Parathormon(PTH)-Gruppe und die Kontrollgruppe wurden 4 bzw. 8 Wochen nach der Operation verglichen. Durch PTH konnte die Knochenbildung im Bereich des Defekts gesteigert werden; dabei wies PTH die stärksten Wirkungen auf das Knochenvolumen pro Gesamtvolumen („bone volume per total volume“, BV/TV), die Trabekelanzahl, Trabekeldicke, Trabekelseparation sowie den gesamten fluoreszenzmarkierten Bereich der Knochenneubildung auf. Außerdem waren eine Hemmung der Expression von CTX (C-terminales Telopeptid vom Typ-I-Kollagen) sowie eine Verstärkung der Expression von Kalzium (Ca2+), Phosphat (P) und alkalischer Knochenphosphatase („bone alkaline phosphatase“, B-ALP) in der PTH-Therapie-Gruppe festzustellen.
Schlussfolgerung
Den Befunden der vorliegenden Studie zufolge ist von einem positiven Effekt von PTH auf die Defektheilung bei ovarektomierten (OVX-)Ratten auszugehen.
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Z-S. Tao, Y-X Lv, W. Cui, Z-L. Huang, K-K. Tu, Q. Zhou, T. Sun, and L. Yang state that there are no conflicts of interest.
All national guidelines on the care and use of laboratory animals have been followed and the necessary approval was obtained from the relevant authorities.
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Tao, ZS., Lv, YX., Cui, W. et al. Effect of teriparatide on repair of femoral metaphyseal defect in ovariectomized rats. Z Gerontol Geriat 49, 423–428 (2016). https://doi.org/10.1007/s00391-015-0949-1
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DOI: https://doi.org/10.1007/s00391-015-0949-1