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Development of a model of benign esophageal stricture in rats: the optimal concentration of sodium hydroxide for stricture formation

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Abstract

Purpose

To investigate the optimal concentration of sodium hydroxide (NaOH) on esophageal stricture formation in rats to establish an animal model of benign esophageal stricture (BES).

Methods

Corrosive esophageal burn was produced by internal application of different concentrations of NaOH to the distal esophagus in rats. As much as 66 male rats were randomly divided into eight groups: Group A (control, n = 6), Group B (sham-operated group, n = 6), Group C (5% NaOH, n = 8), Group D (10% NaOH, n = 8), Group E (20% NaOH, n = 8), Group F (30% NaOH, n = 10), Group G (40% NaOH, n = 14), and Group H (50% NaOH, n = 6). Surviving rats were killed at 28 days. The survival rate, body weight gain, symptoms, and histopathological changes were assessed.

Results

The mortality rate was high in Groups G and H (73 and 67%). The prevalence of symptoms of BES was 43% in Groups D and E, 50% in Group F, 75% in Group G, and 100% in Group H. Statistically significant stricture formation of the esophagus was observed in Groups F and G. The degree of tissue damage was significantly higher in Groups E, F, and G.

Conclusion

A high concentration of NaOH of 30% was required to establish a survivable BES model in rats.

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References

  1. Ferguson D (2005) Evaluation and management of benign esophageal strictures. Dis Esophagus 18:359–364

    Article  CAS  PubMed  Google Scholar 

  2. Türkyilmaz Z, Sönmez K, Karabulut R, Gülbahar O, Poyraz A, Sancak B, Başaklar A (2009) Mitomycin C decreases the rate of stricture formation in caustic esophageal burns in rats. Surgery 145:219–225

    Article  PubMed  Google Scholar 

  3. Cheng Y, Li M, Yang R, Zhang H, Ding Z, Zhuang Q, Jiang Z, Shang K (2003) Restenosis following balloon dilation of benign esophageal stenosis. World J Gastroenterol 9:2605–2608

    PubMed  Google Scholar 

  4. Friedman EM, Deskin R (1994) Caustic ingestion. In: Ronald A, Morris J (eds) Oxford textbook of surgery. Malt, New York, p 2110

    Google Scholar 

  5. Mattos G, Lopes D, Mamede R, Ricz H, Mello-Filho F, Neto J (2006) Effects of time of contact and concentration of caustic agent on generation of injuries. Laryngoscope 116:456–460

    Article  PubMed  Google Scholar 

  6. Baskerville J, Nelson R, Reynolds T, Cohen M (2002) Development of a standardized animal model for the study of alkali ingestion. Vet Hum Toxicol 44:45–47

    PubMed  Google Scholar 

  7. National Research Council, Institute of Laboratory Animal Research, Commission on Life Sciences (eds) (1996) Guide for the care and use of laboratory animals. National Academy Press, Washington, DC

  8. Yukselen V, Vardar E, Yukselen O, Karaoglu AO, Yenisey C, Ozutemiz O (2006) Colchicine in experimental alkaline burns of the rat esophagus: an old drug, a new indication? Pediatr Surg Int 22:363–368

    Article  PubMed  Google Scholar 

  9. Makay O, Yukselen V, Vardar E, Yenisey C, Bicakci T, Ersin S, Ozutemiz O (2007) Role of allopurinol on oxidative stress in caustic burn: cure for stricture? Pediatr Surg Int 23:1105–1112

    Article  PubMed  Google Scholar 

  10. Yukselen V, Vardar E, Yukselen O, Oruc N, Yenisey C, Karaoglu A, Ozutemiz O (2008) Infliximab in experimental alkali burns of the oesophagus in the rat. Acta Gastroenterol Belg 71:21–26

    CAS  PubMed  Google Scholar 

  11. Vardar E, Vardar R, Yukselen V, Makay O, Erkan N, Bayol U, Ozutemiz O (2009) Image-based assessment of esophageal stricture in experimental corrosive esophagitis in animals: an objective, adjunct diagnostic tool. Turk J Gastroenterol 20:3–8

    PubMed  Google Scholar 

  12. Demirbilek S, Bernay F, Rizalar R, Bariş S, Gürses N (1994) Effects of estradiol and progesterone on the synthesis of collagen in corrosive esophageal burns in rats. J Pediatr Surg 29:1425–1428

    Article  CAS  PubMed  Google Scholar 

  13. Apaydin B, Paksoy M, Artiş T, Sahin D, Aki H, Uslu E (2001) Influence of pentoxifylline and interferon-alpha on prevention of stricture due to corrosive esophagitis. An experimental study in the rat. Eur Surg Res 33:225–231

    Article  CAS  PubMed  Google Scholar 

  14. Gehanno P, Guédon C, Marche C, Lallemant Y (1978) Experimental model for the study of caustic stenoses of the oesophagus. Trial of d-penicillamine (author’s transl). Ann Otolaryngol Chir Cervicofac 95:373–378

    CAS  PubMed  Google Scholar 

  15. Gehanno P, Guedon C (1981) Inhibition of experimental esophageal lye strictures by penicillamine. Arch Otolaryngol 107:145–147

    CAS  PubMed  Google Scholar 

  16. Liu AJ, Richardson MA (1985) Effects of N-acetylcysteine on experimentally induced esophageal lye injury. Ann Otol Rhinol Laryngol 94:477–482

    CAS  PubMed  Google Scholar 

  17. Günel E, Cağlayan F, Cağlayan O, Akillioğlu I (1999) Reactive oxygen radical levels in caustic esophageal burns. J Pediatr Surg 34:405–407

    Article  PubMed  Google Scholar 

  18. Bingol-Kologlu M, Tanyel FC, Muftuoglu S, Renda N, Cakar N, Buyukpamukcu N, Hicsonmez A (1999) The preventive effect of heparin on stricture formation after caustic esophageal burns. J Pediatr Surg 34:291–294

    Article  CAS  PubMed  Google Scholar 

  19. Koltuksuz U, Mutus HM, Kutlu R, Ozyurt H, Cetin S, Karaman A, Gurbuz N, Akyol O, Aydin NE (2001) Effects of caffeic acid phenethyl ester and epidermal growth factor on the development of caustic esophageal stricture in rats. J Pediatr Surg 36:1504–1509

    Article  CAS  PubMed  Google Scholar 

  20. Tugay M, Utkan T, Utkan Z (2003) Effects of caustic lye injury to the esophageal smooth muscle reactivity: in vitro study. J Surg Res 113:128–132

    Article  CAS  PubMed  Google Scholar 

  21. Ozel SK, Dagli TE, Yuksel M, Kiyan G, Kotiloglu E (2004) The roles of free oxygen radicals, nitric oxide, and endothelin in caustic injury of rat esophagus. J Pediatr Surg 39:1381–1385

    Article  PubMed  Google Scholar 

  22. Kiyan G, Aktas S, Ozel K, Isbilen E, Kotiloglu E, Dagli T (2004) Effects of hyperbaric oxygen therapy on caustic esophageal injury in rats. J Pediatr Surg 39:1188–1193

    Article  PubMed  Google Scholar 

  23. Somuncu S, Cakmak M, Erdogan S, Caglayan O, Akman H, Kaya M (2005) Trapidil, an inhibitor for phosphodiesterase and platelet-derived-growth factor, ameliorates corrosive esophageal burn in rats. Tohoku J Exp Med 207:203–208

    Article  CAS  PubMed  Google Scholar 

  24. Temir ZG, Karkiner A, Karaca I, Ortac R, Ozdamar A (2005) The effectiveness of sucralfate against stricture formation in experimental corrosive esophageal burns. Surg Today 35:617–622

    Article  CAS  PubMed  Google Scholar 

  25. Guven A, Demirbag S, Uysal B, Topal T, Erdogan E, Korkmaz A, Ozturk H (2008) Effect of 3-amino benzamide, a poly(adenosine diphosphate-ribose) polymerase inhibitor, in experimental caustic esophageal burn. J Pediatr Surg 43:1474–1479

    Article  PubMed  Google Scholar 

  26. Guven A, Gundogdu G, Sadir S, Topal T, Erdogan E, Korkmaz A, Surer I, Ozturk H (2008) The efficacy of ozone therapy in experimental caustic esophageal burn. J Pediatr Surg 43:1679–1684

    Article  PubMed  Google Scholar 

  27. Larios-Arceo F, Ortiz GG, Huerta M, Leal-Cortes C, Saldana JA, Bitzer-Quintero OK, Rodriguez-Reynoso S (2008) Protective effects of melatonin against caustic esophageal burn injury in rats. J Pineal Res 45:219–223

    Article  CAS  PubMed  Google Scholar 

  28. Topaloglu B, Bicakci U, Tander B, Ariturk E, Kilicoglu-Aydin B, Aydin O, Rizalar R, Ayyildiz SH, Bernay F (2008) Biochemical and histopathologic effects of omeprazole and vitamin E in rats with corrosive esophageal burns. Pediatr Surg Int 24:555–560

    Article  PubMed  Google Scholar 

  29. Uguralp S, Irsi C, Aksoy T, Karabulut AB, Kirimlioglu H, Mizrak B (2008) Resveratrol attenuates inflammation and stricture formation in experimental caustic esophageal burns. Pediatr Surg Int 24:425–430

    Article  CAS  PubMed  Google Scholar 

  30. Corduk N, Koltuksuz U, Calli-Demirkan N, Rota S, Abban G, Sarioglu-Buke A (2010) Effects of retinoic acid and zinc on the treatment of caustic esophageal burns. Pediatr Surg Int 26:619–624

    Article  PubMed  Google Scholar 

  31. Bakan V, Garipardic M, Okumus M, Ciralik H, Atli Y, Ozbag D, Tolun FI (2010) The protective effect of erythropoietin on the acute phase of corrosive esophageal burns in a rat model. Pediatr Surg Int 26:195–201

    Article  PubMed  Google Scholar 

  32. Demirbilek S, Aydin G, Yücesan S, Vural H, Bitiren M (2002) Polyunsaturated phosphatidylcholine lowers collagen deposition in a rat model of corrosive esophageal burn. Eur J Pediatr Surg 12:8–12

    Article  CAS  PubMed  Google Scholar 

  33. Ozçelik M, Pekmezci S, Saribeyoğlu K, Unal E, Gümüştaş K, Doğusoy G (2004) The effect of halofuginone, a specific inhibitor of collagen type 1 synthesis, in the prevention of esophageal strictures related to caustic injury. Am J Surg 187:257–260

    Article  PubMed  Google Scholar 

  34. Ekingen G, Ozden M, Sözübir S, Maral H, Müezzinoğlu B, Kahraman H, Güvenç B (2005) Effect of the prostacyclin derivate iloprost in experimental caustic esophageal burn. Pediatr Surg Int 21:441–444

    Article  CAS  PubMed  Google Scholar 

  35. Basaran UN, Eskiocak S, Altaner S, Ture M, Yapar SB (2005) Inhibition of iNOS with S-methylisothiourea was impaired in wound healing in caustic esophageal burn. Int J Pediatr Otorhinolaryngol 69:471–477

    Article  PubMed  Google Scholar 

  36. Arbell D, Udassin R, Koplewitz B, Ohana M, Genina O, Pines M, Nagler A (2005) Prevention of esophageal strictures in a caustic burn model using halofuginone, an inhibitor of collagen type I synthesis. Laryngoscope 115:1632–1635

    Article  PubMed  Google Scholar 

  37. Türkyilmaz Z, Sönmez K, Demirtola A, Karabulut R, Poyraz A, Gülen S, Dinçer S, Başaklar A, Kale N (2005) Mitomycin C prevents strictures in caustic esophageal burns in rats. J Surg Res 123:182–187

    Article  PubMed  Google Scholar 

  38. Yukselen V, Karaoglu AO, Ozutemiz O, Yenisey C, Tuncyurek M (2004) Ketotifen ameliorates development of fibrosis in alkali burns of the esophagus. Pediatr Surg Int 20:429–433

    Article  PubMed  Google Scholar 

  39. Yukselen V, Karaoglu AO, Yenisey C, Tuncyurek M, Ozutemiz O (2005) Trimetazidine reduces the degree of fibrosis in alkali burns of the esophagus. J Pediatr Surg 40:505–509

    Article  PubMed  Google Scholar 

  40. Ocakci A, Coskun O, Tumkaya L, Kanter M, Gurel A, Hosnuter M, Uzun L (2006) Beneficial effects of Ebselen on corrosive esophageal burns of rats. Int J Pediatr Otorhinolaryngol 70:45–52

    Article  PubMed  Google Scholar 

  41. Ocakci A, Kanter M, Cabuk M, Buyukbas S (2006) Role of caffeic acid phenethyl ester, an active component of propolis, against NAOH-induced esophageal burns in rats. Int J Pediatr Otorhinolaryngol 70:1731–1739

    Article  PubMed  Google Scholar 

  42. Cakmak M, Nayci A, Renda N, Erekul S, Gokcora H, Yucesan S (1997) The effect of corticosteroids and pentoxifylline in caustic esophageal burns. A prospective trial in rats. Int Surg 82:371–375

    CAS  PubMed  Google Scholar 

  43. Senturk E, Sen S, Pabuccu E, Unsal C, Meteoglu I (2010) New experimental corrosive esophagitis model in rats. Pediatr Surg Int 26:257–261

    Article  PubMed  Google Scholar 

  44. Henry M, Pelissari C, Carvalho L (2008) Morphological and functional evaluation of distal esophagus of rabbits submitted to esophageal infusion with caustic soda. Acta Cir Bras 23:16–21

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Division of Pediatric Surgery, Department of Surgery, Kobe University Graduate School of Medicine, 7-5-2, Kusunoki-Cho, Chuo-ku, Kobe, 650-0017, Japan

    Yuichi Okata, Chieko Hisamatsu & Eiji Nishijima

  2. Division of Cardiovascular Surgery, Department of Surgery, Kobe University Graduate School of Medicine, Kobe, Japan

    Tomomi Hasegawa & Yutaka Okita

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  1. Yuichi Okata
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  2. Chieko Hisamatsu
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  3. Tomomi Hasegawa
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  4. Eiji Nishijima
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  5. Yutaka Okita
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Correspondence to Yuichi Okata.

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Okata, Y., Hisamatsu, C., Hasegawa, T. et al. Development of a model of benign esophageal stricture in rats: the optimal concentration of sodium hydroxide for stricture formation. Pediatr Surg Int 27, 73–80 (2011). https://doi.org/10.1007/s00383-010-2711-5

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