Abstract
Amplified fragment length polymorphisms (AFLP) markers were developed to assess the genetic variation of populations and clones of Rhopilema esculentum Kishinouye (Scyphozoa, Rhizostomatidae). One hundred and seventy-nine loci from 56 individuals of two hatchery populations and two wild populations were genotyped with five primer combinations. The polymorphic ratio, Shannon’s diversity index and average heterozygosity were 70.3%, 0.346 and 0.228 for the white hatchery population, 74.3%, 0.313, and 0.201 for the red hatchery population, 79.3%, 0.349, and 0.224 for the Jiangsu wild population, and 74.9%, 0.328 and 0.210 for the Penglai wild population, respectively. Thus, all populations had a relatively high level of genetic diversity. A specific band was identified that could separate the white from the red hatchery population. There was 84.85% genetic differentiation within populations. Individual cluster analysis using unweighted pair-group method with arithmetic mean (UPGMA) suggested that hatchery populations and wild populations could be divided. For the hatchery populations, the white and red populations clustered separately; however, for the wild populations, Penglai and Jiangsu populations clustered together. The genetic diversity at the clone level was also determined. Our data suggest that there are relatively high genetic diversities within populations but low genetic differentiation between populations, which may be related to the long-term use of germplasm resources from Jiangsu Province for artificial seeding and releasing. These findings will benefit the artificial seeding and conservation of the germplasm resources.
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References
Chen J, Ding G. 1983. Effect of temperature on the strobilation of jellyfih (Rhopilema esculenta Kishinouye -Scyphozoa, Rhizostomeae). Acta Zool. Sinica, 29: 195–206. (in Chinese with English abstract)
Chen J, Ding G, Liu C. 1984. Effect of light on the strobilation of edible medusa, Rhopilema esculenta Kishinouye (Cnidaria, Scyphozoa). Oceanol. Limnol. Sinica, 15: 310–316. (in Chinese with English abstract)
Custance D. 1967. Studies on strobilation in the Scyphozoa. J. Biol. Educ., 1: 79–81.
Dice L R. 1945. Measures of the amount of ecologic association between species. Ecol., 26: 297–302.
Ding G, Chen J. 1984. The life history of R hopilema esculenta Kishinouye. Journal of Fisheries of China, 15(4): 310–316. (in Chinese with English abstract)
Excoffier L, Laval G, Schneider S. 2005. Arlequin (version 3.0): an integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online, 1: 47–50.
Fetip A, Martinez-Rodriguez G, Piferrer F, Carrillo M, Zanuy S. 2000. AFLP analysis confirms exclusive maternal genomic contribution of meiogynogenetic Sea Bass (Dicentrarchus labrax L.). Mar. Biotechnol., 2: 301–306.
Gao X, Cao J, Dong J, Liu X, He C. 2009. AFLP analysis in jellyfish populations of Rhopilema esculentum and Nemopilema nomurai in liaoning coast. Fisheries Science, 28(2): 75–78. (in Chinese with English abstract)
Liu Y, Li J, Ye N. 2011. Application of AFLP markers for population genetic study on half-smooth tongue sole Cynoglossus semilaevis. Chin. J. Oceanol. Limnol., 29(3): 609–615.
Nei M. 1973. Analysis of gene diversity in subdivided populations. Proc. Nat. Acad. Sci., 70(12): 3 321–3 323.
Nei M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89(3): 583–590.
Qu R, Hou L, Lu H, Li H. 2004. The gene flow of population genetic structure. Hereditas (Beijing), 26(3): 377–382.
Rohlf F J. 2000. Ntsys-pc. Numerical Taxonomy and Multivariate Analysis System. Version 2.10e. Applied Biostatics Inc., New York, USA.
Sambrook J, Russell D W. 2002. Molecular Cloning: A Laboratory Manual. 3rd edn. Science Press, Beijing, China. p.6.4. (in Chinese)
Sun G, Liu X, Yang J, Zhang X, Liu A, Tan F. 2010. Genetic diversity of ITS sequences in farmed and natural Rhopilema esculentum populations. Marine Sciences, 34(10): 90–95. (in Chinese with English abstract)
Vos P, Hogers R, Bleeker M, Reijans M, Lee T, Hornes M, Friters A, Pot J, Paleman J, Kuiper M, Zabeau M. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res., 23: 4 072–4 414.
Wright S. 1951. The genetical structure of population. Ann. Eugen., 15: 323–334.
Yeh F C, Yang R C, Boyle T. 1999. POPGENE, version 1.3.1. Microsoft Window-based Freeware for Population Genetic Analysis. University of Alberta, Edmonton, Canada.
You K, Ma C, Gao H, Li F, Zhang M, Qiu Y, Wang B. 2007. Research on the jellyfish (Rhopilema esculentum Kishinouye) and associated aquaculture techniques in China: current status. Aquacult. Int., 15: 479–488.
Zhou Y, Wang Y, Huang M. 2004. Jellyfish, Rhopilema esculenta Kishinouye, enhancement research in the coastal waters of Zhejiang. Journal of Zhejiang Ocean University (Natural Science), 23(1): 28–30. (in Chinese with English abstract)
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Supported by the Taishan Scholarship of Aquatic Animal Nutrition and Feed and the National Marine Public Welfare Research Project (Nos. 201205025, 201305001)
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Qiao, H., Liu, X., Zhang, X. et al. Genetic diversity of populations and clones of Rhopilema esculentum in China based on AFLP analysis. Chin. J. Ocean. Limnol. 31, 391–397 (2013). https://doi.org/10.1007/s00343-013-2158-3
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DOI: https://doi.org/10.1007/s00343-013-2158-3