Skip to main content
SpringerLink
Log in

Cryopreservation of Chrysanthemum morifolium (Dendranthema grandiflora Ramat.) using different approaches

  • Cell Biology and Morphogenesis
  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Chrysanthemum species are grown both as ornamentals and for the production of pyrethrum. Recent increased production and breeding efforts have raised the need for the conservation of valuable germplasm. Chrysanthemum has been cryopreserved by controlled-rate-freezing as early as 1990. We report here deep-freezing of shoot tips of C. morifolium var. Escort by different technical procedures: controlled-rate-freezing, encapsulation/dehydration, ultra-rapid-freezing by the droplet method and vitrification. While vitrification yielded the highest shoot regeneration rates, the very simple droplet method was also successful in this respect. Droplet freezing was successfully performed with nine cultivars. Our results open the door to the successful use of alternative methods if one method fails to cryopreserve a variety. Furthermore, it enables comparative investigations of genetic stability and cyro-injury to be carried out.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2A–C.
Fig. 3.

Similar content being viewed by others

Abbreviations

BA :

6-Benzylaminopurine

NAA :

α-Naphthaleneacetic acid

References

  • Escobar HR, Debouck D, Roca WM (2000) Development of cassava cryopreservation. In: Engelmann F, Takagi H (eds) Cryopreservation of tropical plant germplasm. JIRCAS International Agricultural Series No. 8, IPGRI, Rome, pp 222–226

  • Fabre J, Dereuddre J (1990) Encapsulation-dehydration a new approach to cryopreservation of Solanum shoot tips. Cryo-Letters 11:413–426

  • Fukai S (1990) Cryopreservation of Chrysanthemum shoot tips. Sci Hortic 45:167–174

    Google Scholar 

  • Fukai S, Goi M, Tanaka M (1991) Cryopreservation of shoot tips of Chrysanthemum morifolium and related species. Euphytica 54:201–204

    Google Scholar 

  • Hitmi A, Barthomeuf C, Sallanon H (2000a) Cryopreservation of Chrysanthemum cinerarifolium shoot tips. J Plant Physiol 156:408–412

    CAS  Google Scholar 

  • Hitmi A, Coudret A, Barthomeuf C, Sallanon H (2000b) Role of intracellular water retention strength in freezing tolerance of Chrysanthemum cinerarifolium Vis. cell cultures. J Plant Physiol 157:47–53

    CAS  Google Scholar 

  • Mandal BB (2000) Cryopreservation of yam apices: a comparative study with three different techniques. In: Engelmann F, Takagi H (eds) Cryopreservation of tropical plant germplasm. JIRCAS International Agricultural Series No. 8, IPGRI, Rome, pp 233–237

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473–497

    CAS  Google Scholar 

  • Panis BK, Vandenbranden H, Schoofs H, Swennen R (1998) Conservation of banana germplasm through cryopreservation. Acta Hortic 461:515–521

    Google Scholar 

  • Sakai A, Kobayashi S, Ojima I (1990) Cryopreservation of nucellar cells of naval orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification. Plant Cell Rep 9:33–33

    Google Scholar 

  • Schäfer-Menuhr A, Mix-Wagner G, Schumacher HM (1997) Cryopreservation of potato cultivars—design of a method for routine application in genebanks. Acta Hortic 447:477–483

    Google Scholar 

Download references

Acknowledgements

The work of Dr. Adela Halmagyi at DSMZ was supported by a Roman-Herzog-Grant of the Humboldt Foundation

Author information

Authors and Affiliations

  1. Institute of Biological Research, Cluj-Napoca, Romania

    A. Halmagyi

  2. Hubert Brandkamp, Jungpflanzen Gärtnerei, In der Flora 6, 46419, Isselburg-Anholt, Germany

    G. Fischer-Klüver

  3. Institute of Crop and Grassland Science, Federal Agricultural Research Centre (FAL), Bundesallee 50, 38116, Braunschweig, Germany

    G. Mix-Wagner

  4. DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b , 38124, Braunschweig, Germany

    A. Halmagyi & H. M. Schumacher

Authors
  1. A. Halmagyi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Fischer-Klüver
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. Mix-Wagner
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. H. M. Schumacher
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to H. M. Schumacher.

Additional information

Communicated by H. Lörz

Rights and permissions

Reprints and permissions

About this article

Cite this article

Halmagyi, A., Fischer-Klüver, G., Mix-Wagner, G. et al. Cryopreservation of Chrysanthemum morifolium (Dendranthema grandiflora Ramat.) using different approaches. Plant Cell Rep 22, 371–375 (2004). https://doi.org/10.1007/s00299-003-0703-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00299-003-0703-9

Keywords

Search

Navigation