Abstract
Molecular studies of some micro-organisms are hampered by the difficulty of obtaining sufficient amounts of nucleic acids. A cloning strategy based on PCR has therefore been used to clone the eburicol 14α-demethylase (CYP51) gene of the obligate fungus Erysiphe graminis f. sp. hordei (Egh) using minute amounts of genomic DNA. The CYP51 gene encodes the enzymatic target of a major group of fungicides. Sequencing CYP51 from different Egh isolates revealed the occurrence of two alleles for this gene. An allele-specific PCR assay was developed to detect each CYP51 allele.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 30 July / 3 October 1998
Rights and permissions
About this article
Cite this article
Délye, C., Bousset, L. & Corio-Costet, MF. PCR cloning and detection of point mutations in the eburicol 14a-demethylase (CYP51) gene from Erysiphe graminis f. sp. hordei, a “recalcitrant” fungus. Curr Genet 34, 399–403 (1998). https://doi.org/10.1007/s002940050413
Issue Date:
DOI: https://doi.org/10.1007/s002940050413