Abstract
Toll-like receptor 4 (TLR4) is a receptor protein that binds pathogen ligands, which are mainly associated with Gram-negative bacteria. The objective of this study was to investigate the association of nucleotide polymorphisms in TLR4 with infectious bovine keratoconjunctivitis (IBK), or pinkeye, incidence in American Angus cattle. Animals with previously calculated breeding values for IBK susceptibility were used to identify two SNPs in TLR4; Int1 (A/G) in intron1 (−26 Ex2 position) and Ex3 (C/T) in exon3 (1,678 position). To investigate the possible role of these SNPs in IBK susceptibility, the disease incidence information was collected on 370 calves raised in Iowa at two time points—June or August (disease season) and October (at weaning) and genotyped using PCR-RFLP protocols. In statistical models including year, pasture management group, and SNP, the Int1 SNP had a significant effect on IBK infection rates both in-season (P < 0.05) and at weaning (P < 0.01), whereas the Ex3 SNP was not significant (P > 0.79) at either time point. Furthermore, the Int1 SNP alone could account for 2.1% of phenotypic variation in IBK infection during the disease season and 3.0% of phenotypic variation in IBK infection at the time of weaning. These data indicate that there is a relationship between Int1 genotype and the rate of IBK infection in American Angus cattle.
This is a preview of subscription content, log in via an institution to check access.
References
Angelos JA, Spinks PQ, Ball LM, George LW (2007) Moraxella bovoculi sp. nov., isolated from calves with infectious bovine keratoconjunctivitis. Int J Syst Evol Microbiol 57:789–795
Casas E, Stone RT (2006) Putative quantitative trait loci associated with the probability of contracting infectious bovine keratoconjunctivitis. J Anim Sci 84:3180–3184
Chang JH, McCluskey PJ, Wakefield D (2006) Toll-like receptors in ocular immunity and the immunopathogenesis of inflammatory eye disease. Br J Ophthalmol 90:103–108
Donovan DM, Kerr DE, Wall RJ (2005) Engineering disease resistant cattle. Transgenic Res 14:563–567
Dracopoli NC, Haines JL, Korf BR, Morton CC, Seidman CC, Seidman JG, Smith DR, Boyle AL (2000) Current protocols in human genetics, vol. 3. Wiley, New York, A.3B.3, A.3C.2
Falconer DS, Mackay TFC (1996) Introduction to quantitative genetics, 4th edn. Longman, Essex, p 10
McGuire K, Jones M, Werling D, Williams JL, Glass EJ, Jann O (2006) Radiation hybrid mapping of all ten characterized bovine toll-like receptors. Anim Genet 37:47–50
Miller SI, Ernst RK, Bader MW (2005) LPS, TLR4 and infectious disease diversity. Nat Rev Microbiol 3:36–46
Mucha R, Bhide MR, Chakurkar EB, Novak M, Mikula I Sr (2009) Toll-like receptors TLR1, TLR2 and TLR4 gene mutations and natural resistance to Mycobacterium avium subsp. paratuberculosis infection in cattle. Vet Immunol Immunopathol 28:381–388
Qu H, Montpetit A, Ge B, Hudson TJ, Polychronakos C (2007) Toward further mapping of the association between the IL2RA locus and type 1 diabetes. Diabetes 56:1174–1176
Roach JC, Glusman G, Rowen L, Kaur A, Purcell MK, Smith KD, Hood LE, Aderem A (2005) The evolution of vertebrate Toll-like receptors. Proc Natl Acad Sci USA 102:9577–9582
Rodriguez JE (2006) IBK (pinkeye) in Black Angus Cattle. Dissertation, Iowa State University, Ames, IA, USA
Sharma BS, Leyva I, Schenkel F, Karrow NA (2006) Association of toll-like receptor four polymorphisms with somatic cell score and lactation persistency in Holstein bulls. J Dairy Sci 89:3626–3635
Snowder GD, VanVleck LD, Cundiff LV, Bennett GL (2005) Genetic and environmental factors associated with incidence of infectious bovine keratoconjunctivitis in preweaned beef calves. J Anim Sci 83:507–518
Tokuhiro S, Yamada R, Chang X, Suzuki A, Kochi Y, Sawada T, Suzuki M, Nagasaki M et al (2003) An intronic SNP in a RUNX1 binding site of SLC22A4, encoding an organic cation transporter, is associated with rheumatoid arthritis. Nat Genet 35:341–348
Trinchieri G, Sher A (2007) Cooperation of Toll-like receptor signals in innate immune defence. Nat Rev Immunol 7:179–190
Wang X, Xu S, Gao X, Ren H, Chen J (2007) Genetic polymorphism of TLR4 gene and correlation with mastitis in cattle. J Genet Genom 34:406–412
Wang X, Luoreng Z, Xu S, Gao X, Li J, Ren H, Chen J (2009) The structure and sequence analysis of TLR4 gene in cattle. Agr Sci China 8:632–637
White SN, Taylor KH, Abbey CA, Gill CA, Womack JE (2003) Haplotype variation in bovine toll-like receptor four and computational prediction of a positively selected ligand-binding domain. Proc Natl Acad Sci USA 100:10364–10369
Acknowledgements
RSK and DK acknowledge the financial support received from Department of Biotechnology, Govt. of India in the form of overseas associateship. Authors recognize Iowa State University Center for Integrated Animal Genomics for financial support of phenotype collections.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kataria, R.S., Tait, R.G., Kumar, D. et al. Association of toll-like receptor four single nucleotide polymorphisms with incidence of infectious bovine keratoconjunctivitis (IBK) in cattle. Immunogenetics 63, 115–119 (2011). https://doi.org/10.1007/s00251-010-0484-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00251-010-0484-6