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Localization of β1-Adrenergic Receptors in the Cochlea and the Vestibular Labyrinth

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Abstract

Sympathetic activation in a “fight or flight reaction” may put the sensory systems for hearing and balance into a state of heightened alert via β1-adrenergic receptors (β1-AR). The aim of the present study was to localize β1-AR in the gerbil inner ear by confocal immunocytochemistry, to characterize β1-AR by Western immunoblots, and to identify β1-AR pharmacologically by measurements of cAMP production. Staining for β1-AR was found in strial marginal cells, inner and outer hair cells, outer sulcus, and spiral ganglia cells of the cochlea, as well as in dark, transitional and supporting cells of the vestibular labyrinth. Receptors were characterized in microdissected inner ear tissue fractions as 55 kDa non-glycosylated species and as 160 kDa high-mannose-glycosylated complexes. Pharmacological studies using isoproterenol, ICI-118551 and CGP-20712A demonstrated β1-AR as the predominant adrenergic receptor in stria vascularis and organ of Corti. In conclusion, β1-AR are present and functional in inner ear epithelial cells that are involved in K+ cycling and auditory transduction, as well as in neuronal cells that are involved in auditory transmission.

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References

  1. S. Angers A. Salahpour M. Bouvier (2001) ArticleTitleBiochemical and biophysical demonstration of GPCR oligomerization in mammalian cells Life Sci 68 2243–2250 Occurrence Handle10.1016/S0024-3205(01)01012-8 Occurrence Handle1:CAS:528:DC%2BD3MXivVaru7s%3D Occurrence Handle11358333

    Article  CAS  PubMed  Google Scholar 

  2. T.R. Bai (1992) ArticleTitleβ2-adrenergic receptors in asthma: a current perspective Lung 170 125–141 Occurrence Handle10.1007/BF00174316 Occurrence Handle1:CAS:528:DyaK3sXitVSisA%3D%3D Occurrence Handle1351969

    Article  CAS  PubMed  Google Scholar 

  3. M. Benallal B.M. Anner (1994) ArticleTitleIdentification of organ-specific glycosylation of a membrane protein in two tissues using lectins Experientia 50 664–668 Occurrence Handle1:CAS:528:DyaK2cXlsFCjtr8%3D Occurrence Handle8033974

    CAS  PubMed  Google Scholar 

  4. R.V. Benya T. Kusui T. Katsuno T. Tsuda S.A. Mantey J.F. Battey R.T. Jensen (2000) ArticleTitleGlycosylation of the gastrin-releasing peptide receptor and its effect on expression, G protein coupling, and receptor modulatory processes Mol. Pharmacol 58 1490–1501 Occurrence Handle1:CAS:528:DC%2BD3cXovFeqsLk%3D Occurrence Handle11093789

    CAS  PubMed  Google Scholar 

  5. V. Boivin R. Jahns S. Gambaryan W. Ness F. Boege M.J. Lohse (2001) ArticleTitleImmunofluorescent imaging of β1- and β2-adrenergic receptors in rat kidney Kidney Int 59 515–531 Occurrence Handle10.1046/j.1523-1755.2001.059002515.x Occurrence Handle1:CAS:528:DC%2BD3MXhtlKisL4%3D Occurrence Handle11168934

    Article  CAS  PubMed  Google Scholar 

  6. T.E. Hebert S. Moffett J.P. Morello T.P. Loisel D.G. Bichet C. Barret M. Bouvier (1996) ArticleTitleA peptide derived from a β2-adrenergic receptor transmembrane domain inhibits both receptor dimerization and activation J. Biol. Chem 271 16384–16392 Occurrence Handle10.1074/jbc.271.24.14280 Occurrence Handle1:CAS:528:DyaK28XktFahtbc%3D Occurrence Handle8663163

    Article  CAS  PubMed  Google Scholar 

  7. I. Hellgren C. Sylven Y. Magnusson (2000) ArticleTitleStudy of the betal adrenergic receptor expression in human tissues: immunological approach Biol. Pharm. Bull 23 700–703 Occurrence Handle1:CAS:528:DC%2BD3cXjvFGnu78%3D Occurrence Handle10864018

    CAS  PubMed  Google Scholar 

  8. I. Herrero J. Sanchez-Prieto (1996) ArticleTitlecAMP-dependent facilitation of glutamate release by β-adrenergic receptors in cerebrocortical nerve terminals J. Biol Chem 271 30554–30560 Occurrence Handle10.1074/jbc.271.48.30554 Occurrence Handle1:CAS:528:DyaK28Xntlamt7o%3D Occurrence Handle8940026

    Article  CAS  PubMed  Google Scholar 

  9. D.J. Jagger J.F. Ashmore (1999) ArticleTitleRegulation of ionic currents by protein kinase A and intracellular calcium in outer hair cells isolated from the guinea-pig cochlea Pfluegers Arch 437 409–416 Occurrence Handle10.1007/s004240050795 Occurrence Handle1:CAS:528:DyaK1MXjtlGgtg%3D%3D

    Article  CAS  Google Scholar 

  10. D.J. Jagger J.F. Ashmore (1999) ArticleTitleThe fast activating potassium current, Ik,f, in guinea-pig inner hair cells is regulated by protein kinase A Neurosci. Lett 263 145–148 Occurrence Handle10.1016/S0304-3940(99)00128-7 Occurrence Handle1:CAS:528:DyaK1MXitlKisrs%3D Occurrence Handle10213156

    Article  CAS  PubMed  Google Scholar 

  11. B.M. Johnstone R. Patuzzi J. Syka E. Sykova (1989) ArticleTitleStimulus-related potassium changes in the organ of Corti of guinea-pig J.Physiol 408 77–92 Occurrence Handle1:STN:280:BiaA1c3jvVY%3D Occurrence Handle2778743

    CAS  PubMed  Google Scholar 

  12. E.A. Laurikainen T. Ren J.M. Miller A.L. Nuttall W.S. Quirk (1997) ArticleTitleThe tonic sympathetic input to the cochlear vasculature in guinea pig Hear Res 105 141–145 Occurrence Handle10.1016/S0378-5955(96)00198-0 Occurrence Handle1:STN:280:ByiB3sjjtlc%3D Occurrence Handle9083811

    Article  CAS  PubMed  Google Scholar 

  13. J.H. Lee T. Chiba D.C. Marcus (2001) ArticleTitleP2X2 receptor mediates stimulation of parasensory cation absorption by cochlear outer sulcus cells and vestibular transitional cells J. Neurosci 21 9168–9174 Occurrence Handle1:CAS:528:DC%2BD3MXoslyiurY%3D Occurrence Handle11717350

    CAS  PubMed  Google Scholar 

  14. L.V. Lopes R.A. Cunha B. Kull B.B. Fredholm J.A. Ribeiro (2002) ArticleTitleAdenosine A2A receptor facilitation of hippocampal synaptic transmission is dependent on tonic A1 receptor inhibition Neuroscience 112 319–329 Occurrence Handle10.1016/S0306-4522(02)00080-5 Occurrence Handle1:CAS:528:DC%2BD38XktVarur0%3D Occurrence Handle12044450

    Article  CAS  PubMed  Google Scholar 

  15. P.G. Milhaud S.R. Pondugula J.H. Lee M. Herzog J. Lehouelleur P. Wangemann A. Sans D.C. Marcus (2002) ArticleTitleChloride secretion by semicircular canal duct epithelium is stimulated via beta 2-adrenergic receptors Am. J. Physiol 283 C1752–C1760 Occurrence Handle1:CAS:528:DC%2BD38XpslGjtLk%3D

    CAS  Google Scholar 

  16. E. Rands M.R. Candelore A.H. Cheung W.S. Hill C.D. Strader R.A. Dixon (1990) ArticleTitleMutational analysis of β-adrenergic receptor glycosylation J. Biol. Chem 265 10759–10764 Occurrence Handle1:CAS:528:DyaK3cXkvFWmsLY%3D Occurrence Handle2162359

    CAS  PubMed  Google Scholar 

  17. D.A. Rathz K.M. Brown L.A. Kramer S.B. Liggett (2002) ArticleTitleAmino Acid 49 polymorphisms of the human β1-adrenergic receptor affect agonist-promoted trafficking J. Cardiovasc. Pharmacol 39 155–160 Occurrence Handle10.1097/00005344-200202000-00001 Occurrence Handle1:CAS:528:DC%2BD38XosFKnsw%3D%3D Occurrence Handle11791000

    Article  CAS  PubMed  Google Scholar 

  18. C.D. Rios B.A. Jordan I. Gomes L.A. Devi (2001) ArticleTitleG-protein-coupled receptor dimeri-zation: modulation of receptor function Pharmacol. Ther 92 71–87 Occurrence Handle10.1016/S0163-7258(01)00160-7 Occurrence Handle1:CAS:528:DC%2BD38XitlejsrY%3D Occurrence Handle11916530

    Article  CAS  PubMed  Google Scholar 

  19. S. Schimanski M.A. Scofield P. Wangemann (2001) ArticleTitleFunctional β2-adrenergic receptors are present in nonstrial tissues of the lateral wall in the gerbil cochlea Audiol. Neurootol 6 124–131 Occurrence Handle10.1159/000046819 Occurrence Handle1:CAS:528:DC%2BD3MXmtF2qsrY%3D Occurrence Handle11474138

    Article  CAS  PubMed  Google Scholar 

  20. H. Spoendlin W. Lichtensteiger (1966) ArticleTitleThe adrenergic innervation of the labyrinth Acta Otolaryngol 61 423–434 Occurrence Handle1:STN:280:CCiD3M%2FjsFY%3D Occurrence Handle5915897

    CAS  PubMed  Google Scholar 

  21. P. Valli G. Zucca L. Botta (1990) ArticleTitlePerilymphatic potassium changes and potassium homeostasis in isolated semicircular canals of the frog J. Physiol 430 585–594 Occurrence Handle1:STN:280:By6B3c3ktFU%3D Occurrence Handle2086775

    CAS  PubMed  Google Scholar 

  22. P. Wangemann (2002) ArticleTitleK+ cycling and the endocochlear potential Hear Res 165 1–9 Occurrence Handle10.1016/S0378-5955(02)00279-4 Occurrence Handle1:CAS:528:DC%2BD38XjvVWjtr0%3D Occurrence Handle12031509

    Article  CAS  PubMed  Google Scholar 

  23. P. Wangemann J. Liu M. Shimozono S. Schimanski M.A. Scofield (2000) ArticleTitleK+ secretion in strial marginal cells is stimulated via β1-adrenergic receptors but not via β2-adrenergic or vasopressin receptors J. Membrane. Biol 175 191–202 Occurrence Handle10.1007/s002320001067 Occurrence Handle1:CAS:528:DC%2BD3cXktl2ht78%3D

    Article  CAS  Google Scholar 

  24. P. Wangemann J. Liu M. Shimozono M.A. Scofield (1999) ArticleTitleβ1-adrenergic receptors but not β2-adrenergic or vasopressin receptors regulate K+ secretion in vestibular dark cells of the inner ear J. Membrane. Biol 170 67–77 Occurrence Handle10.1007/s002329900538 Occurrence Handle1:CAS:528:DyaK1MXksFKrur4%3D

    Article  CAS  Google Scholar 

  25. D.S. Whitlon R. Szakaly M.A. Greiner (2001) ArticleTitleCryoembedding and sectioning of cochleas for immunocytochemistry and in situ hybridization Brain. Res. Brain Res. Protoc 6 159–166 Occurrence Handle10.1016/S1385-299X(00)00048-9 Occurrence Handle1:STN:280:DC%2BD3M3osF2guw%3D%3D Occurrence Handle11223415

    Article  CAS  PubMed  Google Scholar 

  26. M. Wiederholt D.K. Schmidt R. Eggebrecht J. Zimmermann F.H. Fischer (1983) ArticleTitleAdrenergic regulation of sodium and chloride transport in the isolated cornea of rabbit and man Graefes Arch. Clin. Exp Ophthalmol 220 240–244 Occurrence Handle1:CAS:528:DyaL3sXlvVygu7o%3D Occurrence Handle6138298

    CAS  PubMed  Google Scholar 

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Acknowledgments

The authors thank Cindy Chard-Bergstrom for her helpful recommendations in immunohistochemistry and Lili Maleki for reading the manuscript. The support by the Research Grant NIH-ROI-DC01098 from the National Institute on Deafness and Other Communication Disorders, National Institute of Health, is gratefully acknowledged.

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  1. Cell Physiology Laboratory, Anatomy & Physiology Department, Kansas State University, Manhattan, KS, 66506, USA

    C. Fauser, S. Schimanski & P. Wangemann

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  1. C. Fauser
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  2. S. Schimanski
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  3. P. Wangemann
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Correspondence to P. Wangemann.

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Fauser, C., Schimanski, S. & Wangemann, P. Localization of β1-Adrenergic Receptors in the Cochlea and the Vestibular Labyrinth. J Membrane Biol 201, 25–32 (2004). https://doi.org/10.1007/s00232-004-0703-x

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  • DOI: https://doi.org/10.1007/s00232-004-0703-x

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