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Quantitative analysis of urinary phospholipids found in patients with breast cancer by nanoflow liquid chromatography–tandem mass spectrometry: II. Negative ion mode analysis of four phospholipid classes

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Abstract

Analysis was performed on four different categories of phospholipids (phosphatidylserine (PS), phosphatidylinositol (PI), phosphatidylglycerol (PG), and phosphatidic acid (PA)) from urine in patients with breast cancer. This quantitative analysis was conducted using nanoflow liquid chromatography–electrospray ionization–tandem mass spectrometry (nLC-ESI-MS-MS). This study shows the profiling of the phospholipids (PLs) that can be identified by the negative ion mode of MS. A previous study (Kim et al. Anal. Bioanal. Chem. 393:1649, 21) focused on only two PL classes: phosphatidylcholines (PCs) and phosphatidylethanolamines (PEs) and were identified by positive ion mode. PLs were extracted by lyophilization of 1 mL of urine from both healthy normal females and breast cancer patients before and after surgery. Separation of PLs was performed by nLC followed by structural identification of PLs using data-dependent collision-induced dissociation. A total of 34 urinary PL molecules (12 PSs, 12 PIs, four PGs, and six PAs) were quantitatively examined. Among the four PL categories examined in this study, most PL classes showed an increase in the total amounts in the cancer patients, yet PIs exhibited some decreases. The present study suggests that the lipid composition found in the urine of breast cancer patients can be utilized for the possible development of disease markers, when the analysis is performed with negative ion mode of nLC-ESI-MS-MS.

Calibration curves of the concentration dependency (solid line) of each acyl chain length of PS standard deduced from the relationship (dotted lines) between relative peak area (vs. IS) and acyl chain length

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References

  1. Brouwers JFHM, Vernooji EAAM, Tielens AGM, van Golde LMG (1999) J Lipid Res 40:164–169

    CAS  Google Scholar 

  2. Wright MM, Howe AG, Zaremberg V (2004) Biochem Cell Biol 82:18–26

    Article  CAS  Google Scholar 

  3. Wolf C, Quinn PJ (2008) Prog Lipid Res 47:15–36

    CAS  Google Scholar 

  4. Kuksis A, Myher JJ, Marai L (1984) J Am Oil Chem Soc 61:1582–1589

    Article  CAS  Google Scholar 

  5. Heller DN, Murphy CM, Cotter RJ, Fenselau C, Uy OM (1988) Anal Chem 60:2787–2791

    Article  CAS  Google Scholar 

  6. Fuchs B, Schiller J, Süβ R, Schürenburg M, Suckau D (2007) Anal Bioanal Chem 389:827–834

    Article  CAS  Google Scholar 

  7. Rohlfing A, Muthing J, Pojlentz G, Distler I, Peter-Katalinc J, Berkenkamp S, Dreisewerd K (2007) Anal Chem 79:5793–5808

    Article  CAS  Google Scholar 

  8. Helmy F, Rothenbacher F, Nosavanh L, Lowery J, Juracka A (2007) J Planar Chromatogr Modern TLC 20:209–215

    Article  CAS  Google Scholar 

  9. Murray KE, Fried B, Sherma J (2007) Acta Chromatographica 18:190–198

    CAS  Google Scholar 

  10. Kaduce TL, Norton KC, Spector AA (1983) J Lipid Res 24:1398–1404

    CAS  Google Scholar 

  11. He FQ, Wang CT, Li XK, Liu XQ (2007) J Anal Chem 62:369–372

    Article  CAS  Google Scholar 

  12. Kim HY, Wang TCL, Ma YC (1994) Anal Chem 66:3977–3982

    Article  CAS  Google Scholar 

  13. Koivusalo M, Haimi P, Heikinheimo L, Kostiainen R, Somerharaju P (2001) J Lipid Res 42:663–672

    Google Scholar 

  14. Hsu FF, Turk J (2001) J Am Soc Mass Spectrom 12:1036–1043

    Article  CAS  Google Scholar 

  15. Hsu FF, Turk J (2001) J Am Soc Mass Spectrom 11:986–999

    Article  Google Scholar 

  16. Hsu FF, Turk J (2005) J Am Soc Mass Spectrom 16:1510–1522

    Article  CAS  Google Scholar 

  17. Taguchi R, Houjou T, Nakanishi H, Yamazaki T, Ishida M, Imagawa M, Shimizu T (2005) J Chromatogr B 823:26–36

    Article  CAS  Google Scholar 

  18. Bang DY, Kang D, Moon MH (2006) J Chromatogr A 1104:222–229

    Article  CAS  Google Scholar 

  19. Bang DY, Ahn E, Moon MH (2007) J Chromatogr B 852:268–277

    Article  CAS  Google Scholar 

  20. Kim H, Ahn E, Moon MH (2008) Analyst 133:1656–1663

    Article  CAS  Google Scholar 

  21. Kim H, Min HK, Kong G, Moon MH (2009) Anal Bioanal Chem 393:1649–1656

    Article  CAS  Google Scholar 

  22. Hsu FF, Turk J (2000) J Am Mass Spectrom 11:797–803

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by a grant (2008-03136) from the Korea Ministry of Science & Technology (MOST) and partly by a grant from the Korea Science and Engineering Foundation through the Center for Bioactive Molecular Hybrids (CBMH).

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Authors and Affiliations

  1. Department of Chemistry, Yonsei University, Seoul, 120-749, South Korea

    Hye Kyeong Min & Myeong Hee Moon

  2. Department of Pathology, College of Medicine, Hanyang University, Seoul, 133-791, South Korea

    Gu Kong

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  1. Hye Kyeong Min
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  2. Gu Kong
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  3. Myeong Hee Moon
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Correspondence to Myeong Hee Moon.

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Min, H.K., Kong, G. & Moon, M.H. Quantitative analysis of urinary phospholipids found in patients with breast cancer by nanoflow liquid chromatography–tandem mass spectrometry: II. Negative ion mode analysis of four phospholipid classes. Anal Bioanal Chem 396, 1273–1280 (2010). https://doi.org/10.1007/s00216-009-3292-9

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  • DOI: https://doi.org/10.1007/s00216-009-3292-9

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