Abstract
Introduction and hypothesis
The objective was to measure the effects of peroxisome proliferator-activated receptor delta (PPAR-delta) agonist and zinc sulfate (ZS) on vaginal smooth muscle cell (SMC) proliferation, tropoelastin production, and collagen production.
Methods
SMC cultures were performed from vaginal wall biopsies and were incubated with ZS and PPAR-delta agonist GW501516 (GW). Cell proliferation was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Supernatants and cell lysates were collected. Tropoelastin production was measured by Fastin Elastin Assay and collagen was measured with Sircol Collagen Assay.
Results
SMC proliferation was similar with 20 μM ZS or 10 nM GW compared to control. Tropoelastin production was significantly increased by 20 μM ZS and by 10 nM GW. Cell culture surface deposited elastin production was significantly increased by 20 μM ZS and by the combination of 20 μM ZS with 10 nM GW, and collagen production was significantly increased by 10 nM GW and by the combination of 20 μM ZS with 10 nM GW.
Conclusions
PPAR-delta agonist and ZS increases vaginal SMC tropoelastin and collagen production.
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References
Alperin M, Moalli PA (2006) Remodeling of vaginal connective tissue in patients with prolapse. Curr Opin Obstet Gynecol 18:544–550
Budatha M, Roshanravan S, Zheng Q et al (2011) Extracellular matrix proteases contribute to progression of pelvic organ prolapse in mice and humans. J Clin Invest 121:2048–2059
Chen B, Yeh J (2011) Alterations in connective tissue metabolism in stress incontinence and prolapse. J Urol 186:1768–1772
Lin SY, Tee YT, Ng SC, Chang H, Lin P, Chen GD (2007) Changes in the extracellular matrix in the anterior vagina of women with or without prolapse. Int Urogynecol J Pelvic Floor Dysfunct 18:43–48
Stika CS (2010) Atrophic vaginitis. Dermatol Ther 23:514–522
Tinelli A, Malvasi A, Rahimi S et al (2010) Age-related pelvic floor modifications and prolapse risk factors in postmenopausal women. Menopause 17:204–212
Prasad AS (2008) Zinc in human health: effect of zinc on immune cells. Mol Med 14:353–357
Kelkar MA, Khar SK, Mandakhot VM (1989) Studies on antepartum prolapse of the vagina in buffalo–plasma trace element concentrations. Arch Exp Veterinarmed 43:315–318
Taneja SK, Kaur R (1990) Pathology of ovary, uterus, vagina and gonadotrophs of female mice fed on Zn-deficient diet. Indian J Exp Biol 28:1058–1065
Honoré LH, Salkie ML, Jajczay FL (1986) The influence of anatomical site and hormonal status on the copper and zinc levels of human uterine smooth muscle. Clin Biochem 19:46–48
Sznaidman ML, Haffner CD, Maloney PR et al (2003) Novel selective small molecule agonists for peroxisome proliferator-activated receptor delta (PPARdelta)–synthesis and biological activity. Bioorg Med Chem Lett 13:1517–1521
Ham SA, Kim HJ, Kang ES et al (2010) PPARdelta promotes wound healing by up-regulating TGF-beta1-dependent or -independent expression of extracellular matrix proteins. J Cell Mol Med 14:1747–1759
Peters JM, Gonzalez FJ (2009) Sorting out the functional role(s) of peroxisome proliferator-activated receptor-beta/delta (PPARbeta/delta) in cell proliferation and cancer. Biochim Biophys Acta 1796:230–241
Kim HJ, Ham SA, Kim SU et al (2008) Transforming growth factor-beta1 is a molecular target for the peroxisome proliferator-activated receptor delta. Circ Res 102:193–200
Takacs P, Zhang Y, Yavagal S, Candiotti K, Chakhtoura N, Medina CA (2012) TGF-beta 1 is a potential regulator of vaginal tropoelastin production. Int Urogynecol J 23:357–363
Takacs P, Zhang Y, Jaramillo S, Bardawil T, Candiotti K, Medina CA (2010) The effects of estrogen, progesterone and polypropylene mesh on vaginal smooth muscle cell proliferation. J Smooth Muscle Res 46:9–15
Koyano Y, Hejna M, Flechtenmacher J, Schmid TM, Thonar EJ, Mollenhauer J (1996) Collagen and proteoglycan production by bovine fetal and adult chondrocytes under low levels of calcium and zinc ions. Connect Tissue Res 34:213–225
Mahoney MG, Brennan D, Starcher B et al (2009) Extracellular matrix in cutaneous ageing: the effects of 0.1 % copper-zinc malonate-containing cream on elastin biosynthesis. Exp Dermatol 18:205–211
Grommes J, Binnebösel M, Klink CD et al (2011) Balancing zinc deficiency leads to an improved healing of colon anastomosis in rats. Int J Colorectal Dis 26:295–301
Alperin M, Feola A, Meyn L, Duerr R, Abramowitch S, Moalli P (2010) Collagen scaffold: a treatment for simulated maternal birth injury in the rat model. Am J Obstet Gynecol 202(6):589.e1–589.e8
Ozdemir S, Ozis ES, Gulpinar K et al (2011) The value of copper and zinc levels in hernia formation. Eur J Clin Invest 41:285–290
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Takacs, P., Zhang, Y., Candiotti, K. et al. Effects of PPAR-delta agonist and zinc on vaginal smooth muscle cells collagen and tropoelastin production. Int Urogynecol J 23, 1775–1779 (2012). https://doi.org/10.1007/s00192-012-1807-y
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DOI: https://doi.org/10.1007/s00192-012-1807-y