Skip to main content
SpringerLink
Log in

Genetic characterization and fine mapping of the novel Phytophthora resistance gene in a Chinese soybean cultivar

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Phytophthora root rot (PRR), caused by Phytophthora sojae Kaufmann & Gerdemann, is one of the most destructive diseases of soybean [Glycine max (L.) Merr.]. Deployment of resistance genes is the most economical and effective way of controlling the disease. The soybean cultivar ‘Yudou 29’ is resistant to many P. sojae isolates in China. The genetic basis of the resistance in ‘Yudou 29’ was elucidated through an inheritance study and molecular mapping. In response to 25 P. sojae isolates, ‘Yudou 29’ displayed a new resistance reaction pattern distinct from those of differentials carrying known Rps genes. A population of 214 F2:3 families from a cross between ‘Jikedou 2’ (PRR susceptible) and ‘Yudou 29’ was used for Rps gene mapping. The segregation fit a ratio of 1:2:1 for resistance:segregation:susceptibility within this population, indicating that resistance in ‘Yudou 29’ is controlled by a single dominant gene. This gene was temporarily named RpsYD29 and mapped on soybean chromosome 03 (molecular linkage group N; MLG N) flanked by SSR markers SattWM82-50 and Satt1k4b at a genetic distance of 0.5 and 0.2 cM, respectively. Two nucleotide binding site-leucine rich repeat (NBS-LRR) type genes were detected in the 204.8 kb region between SattWM82-50 and Satt1k4b. These two genes showed high similarity to Rps1k in amino acid sequence and could be candidate genes for PRR resistance. Based on the phenotype reactions and the physical position on soybean chromosome 03, RpsYD29 might be a novel allele at, or a novel gene tightly linked to, the Rps1 locus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Allen GC, Flores-Vergara MA, Krasynanski S, Kumar S, Thompson WF (2006) A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide. Nat Protoc 1:2320–2325

    Article  PubMed  CAS  Google Scholar 

  • Bernard RL, Cremeens CR (1981) An allele at the Rps1 locus from the variety Kingwa. Soybean Genet Newsl 8:40–42

    Google Scholar 

  • Bernard RL, Smith PE, Kaufmann MJ, Schmitthenner AF (1957) Inheritance of resistance to Phytophthora root rot and stem rot in the soybean. Agron J 49:391

    Article  Google Scholar 

  • Buzzell RI, Anderson TR (1992) Inheritance and race reaction of a new soybean Rps1 allele. Plant Dis 76:600–601

    Article  Google Scholar 

  • Chen QH, Wang QY, Wang YC, Zheng XB (2004) Identification and sequencing of ribosomal DNA-ITS of Phytophthora sojae in Fujian. Acta Phytopathol Sinica 34:112–116

    Google Scholar 

  • Chen XL, Zhu ZD, Wang XM, Xiao YN, Wu XF (2008) Postulation of Phytophthora resistance genes in soybean cultivars or lines. Sci Agric Sinica 41:1227–1234

    CAS  Google Scholar 

  • Cui KL, Yin WX, Tang QH, Dong SM, Zheng XB, Zhang ZG, Wang YC (2010) Distribution, pathotypes, and metalaxyl sensitivity of Phytophthora sojae from Heilongjiang and Fujian provinces in China. Plant Dis 94:881–884

    Article  Google Scholar 

  • Dorrance AE, McClure SA, St Martin SK (2003) Effect of partial resistance on Phytophthora stem rot incidence and yield of soybean in Ohio. Plant Dis 87:308–312

    Article  Google Scholar 

  • Fan AY, Wang XM, Fang XP, Wu XF, Zhu ZD (2009) Molecular identification of Phytophthora resistance gene in soybean cultivar Yudou 25. Acta Agron Sinica 35:1844–1850

    Article  CAS  Google Scholar 

  • Gao H, Bhattacharyya MK (2008) The soybean-Phytophthora resistance locus Rps1-k encompasses coiled coil-nucleotide binding-leucine rich repeat-like genes and repetitive sequences. BMC Plant Biol 8:1–14

    Article  CAS  Google Scholar 

  • Gao H, Narayanan NN, Ellison L, Bhattacharyya MK (2005) Two classes of highly similar coiled coil-nucleotide binding-leucine rich repeat genes isolated from the Rps1-k locus encode Phytophthora resistance in soybean. Mol Plant Microbe Interact 18:1035–1045

    Article  PubMed  CAS  Google Scholar 

  • Gordon SG, St Martin SK, Dorrance AE (2006) Rps8 maps to a resistance gene rich region on soybean molecular linkage group F. Crop Sci 46:168–173

    Article  CAS  Google Scholar 

  • Grau CR, Dorrance AE, Bond J, Russin J (2004) Fungal diseases. In: Boerma HR, Specht JE (eds) Soybeans: Improvement, production and uses. Agronomy Monograph, 3rd edn. American Society of Agronomy, Madison, pp 679–763

    Google Scholar 

  • Haas JH, Buzzell RI (1976) New races 5 and 6 of Phytophthora megasperma var. sojae and differential reactions of soybean cultivars for races 1 and 6. Phytopathology 66:1361–1362

    Article  Google Scholar 

  • Kuan TL, Erwin DC (1980) Formae speciales differentiation of Phytophthora megasperma isolates from soybeans and alfalfa. Phytopathology 70:333–338

    Article  Google Scholar 

  • Kyle DE, Nickell CD, Nelson RL, Pedersen WL (1998) Response of soybean accessions from provinces in southern China to Phytophthora sojae. Plant Dis 82:555–559

    Article  Google Scholar 

  • Lohnes DG, Schmitthenner AF, Nickell CD (1994) Incidence and distribution of Phytophthora resistance in new soybean germplasm from China. Agronomy Abstracts 217

  • Lohnes DG, Nickell CD, Schmitthenner AF (1996) Origin of soybean alleles for Phytophthora resistance in China. Crop Sci 36:1689–1692

    Article  Google Scholar 

  • Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832

    Article  PubMed  CAS  Google Scholar 

  • Mueller EH, Athow KL, Laviolette FA (1978) Inheritance of resistance to four physiologic races of Phytophthora megasperma var. sojae. Phytopathology 68:1318–1322

    Article  Google Scholar 

  • Ploper LD, Athow KL, Laviolette FA (1985) A new allele at Rps3 locus for resistance to Phytophthora megasperma f. sp. glycinea in soybean. Phytopathology 75:690–694

    Article  Google Scholar 

  • Rohlf F (2000) NTSYS-PC numerical taxonomy and multivariate analysis system ver 2.11L. Appl Biostat, New York

    Google Scholar 

  • Sandhu D, Schallock KG, Rivera-Velez N, Lundeen P, Cianzio S, Bhattacharyya MK (2005) Soybean Phytophthora resistance gene Rps8 maps closely to the Rps3 region. J Hered 96:536–541

    Article  PubMed  CAS  Google Scholar 

  • Schmitthenner AF (1999) Phytophthora rot of soybean. In: Hartman GL, Sinclair JB, Rupe JC (eds) Compendium of soybean diseases, 4th edn. The American Phytopathological Society Press, St Paul, pp 39–42

    Google Scholar 

  • Schmitthenner AF, Hobe M, Bhat RG (1994) Phytophthora sojae races in Ohio over a 10-year interval. Plant Dis 78:269–276

    Article  Google Scholar 

  • Schmutz J, Cannon SB, Schlueter J, Ma J, Mitros T, Nelson W, Hyten DL, Song Q, Thelen JJ, Cheng J, Xu D, Hellsten U, May GD, Yu Y, Sakurai T, Umezawa T, Bhattacharyya MK, Sandhu D, Valliyodan B, Lindquist E, Peto M, Grant D, Shu S, Goodstein D, Barry K, Futrell-Griggs M, Ab-ernathy B, Du J, Tian Z, Zhu L, Gill N, Joshi T, Libault M, Sethuraman A, Zhang XC, Shinozaki K, Nguyen HT, Wing RA, Cregan PB, Specht J, Grimwood J, Rokhsar D, Stacey G, Shoemaker RC, Jackson SA (2010) Genome sequence of the palaeopolyploid soybean. Nature 463:178–183

    Article  PubMed  CAS  Google Scholar 

  • Shen CY, Su YC (1991) Discovery and preliminary studies of Phytophthora megasperma on soybean in China. Acta Phytopathol Sinica 21:298

    Google Scholar 

  • Slaminko TL, Bowen CR, Hartman GL (2010) Multi-Year evolution of commercial soybean cultivars for resistance to Phytophthora sojae. Plant Dis 94:368–371

    Article  Google Scholar 

  • Sugimoto T, Yoshida S, Watanabe K, Aino M, Kanto T, Maekawa K, Irie K (2008) Identification of SSR markers linked to the Phytophthora resistance gene Rps1-d in soybean. Plant Breed 127:154–159

    Article  CAS  Google Scholar 

  • Sugimoto T, Yoshida S, Kaga A, Hajika M, Watanabe K, Aino M, Tatsuda K, Yamamoto R, Matoh T, Walker DR, Biggs AR, Ishimoto M (2011) Genetic analysis and Identification of DNA markers linked to a novel Phytophthora sojae resistance gene in the Japanese soybean cultivar Waseshiroge. Euphytica 182:133–145

    Article  CAS  Google Scholar 

  • Sugimoto T, Kato M, Yoshida S, Matsumoto I, Kobayashi T, Kaga A, Hajika M, Yamamoto R, Watanabe K, Aino M, Matoh T, David R, Walker DR, Biggs AR, Ishimoto M (2012) Pathogenic diversity of Phytophthora sojae and breeding strategies to develop Phytophthora-resistant soybeans. Breed Sci 61:511–522

    Article  PubMed  CAS  Google Scholar 

  • Sun S, Wu XL, Zhao JM, Wang YC, Tang QH, Yu DY, Gai JY, Xing H (2011) Characterization and mapping of RpsYu25, a novel resistance gene to Phytophthora sojae. Plant Breed 130:139–143

    Article  CAS  Google Scholar 

  • Tang QH, Cui LK, Li DL, Dai TT, Yin WX, Dong SM, Xing H, Zheng XB, Wang YC (2010) Resistance evaluation of soybean germplasm from Huanghuai Valley to Phytophthora Root Rot. Sci Agric Sinica 43:2246–2252

    Google Scholar 

  • Tyler BM (2007) Phytophthora sojae: root rot pathogen of soybean and model oomycete. Mol Plant Pathol 8:1–8

    Article  PubMed  CAS  Google Scholar 

  • Van Ooijen JW (2006) JoinMap 4 Software for the calculation of genetic linkage maps in experimental populations. Kyazma B.V., Wageningen

    Google Scholar 

  • Weng C, Yu K, Anderson TR, Poysa V (2001) Mapping genes conferring resistance to Phytophthora root rot of soybean, Rps1a and Rps7. J Hered 92:442–446

    Article  PubMed  CAS  Google Scholar 

  • Wrather JA, Stienstra WC, Koenning SR (2001) Soybean disease loss estimates for the United States from 1996 to 1998. Can J Plant Pathol 23:122–131

    Article  Google Scholar 

  • Xia CJ, Zhang JQ, Wang XM, Wu XF, Liu ZX, Zhu ZD (2011) Analyses of resistance genes to Phytophthora root rot in soybean germplasm. Chin J Oil Crop Sci 33:396–401

    CAS  Google Scholar 

  • Yao HY, Wang XM, Wu XF, Xiao YN, Zhu ZD (2010) Molecular mapping of Phytophthora resistance gene in soybean cultivar Zaoshu18. J Plant Genet Resour 11:213–217

    CAS  Google Scholar 

  • Yu AL, Xu PF, Wang JS, Zhang SZ, Wu JJ, Li WB, Chen WY, Li NH, Fan SJ, Wang X, Jiang LY (2010) Genetic analysis and SSR mapping of gene resistance to Phytophthora sojae race 1 in soybean cv Suinong 10. Chin J Oil Crop Sci 32:462–466

    Google Scholar 

  • Zhang SZ, Xu PF, Wu JJ, Xue AG, Zhang JX, Li WB, Chen C, Chen WY, Lv HY (2010) Races of Phytophthora sojae and their virulences on soybean cultivars in Heilongjiang, China. Plant Dis 94:87–91

    Article  Google Scholar 

  • Zhu ZD, Wang HB, Wang XM, Chang RZ, Wu XF (2003) Distribution and virulence diversity of Phytophthora sojae in China. Sci Agric Sinica 36:793–799

    Google Scholar 

  • Zhu ZD, Huo YL, Wang XM, Huang JB, Wu XF (2006) Screening for resistance sources to Phytophthora root rot in soybean. J Plant Genet Resour 7:24–30

    Google Scholar 

  • Zhu ZD, Huo YL, Wang XM, Huang JB, Wu XF (2007) Molecular identification of a novel Phytophthora resistance gene in soybean. Acta Agron Sinica 33:154–157

    CAS  Google Scholar 

Download references

Acknowledgments

The work was supported by the Special Fund for Agro-scientific Research in the Public Interest (3-20, 201303018) and the Program of Protection of Crop Germplasm Resources (NB2010-2130135-25-14) from the Ministry of Agriculture of the People’s Republic of China. We thank Dr. Schmitthenner at the Ohio State University, USA, for providing the cultivars/lines carrying a single Rps gene at the Rps1 to Rps8 loci and the susceptible control cultivar Williams.

Author information

Authors and Affiliations

  1. MOA Key Lab of Soybean Biology (Beijing), the National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing, 100081, People’s Republic of China

    Jiqing Zhang, Changjian Xia, Xiaoming Wang, Canxing Duan, Suli Sun, Xiaofei Wu & Zhendong Zhu

Authors
  1. Jiqing Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Changjian Xia
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiaoming Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Canxing Duan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Suli Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Xiaofei Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Zhendong Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhendong Zhu.

Additional information

Communicated by D. Lightfoot.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 35 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, J., Xia, C., Wang, X. et al. Genetic characterization and fine mapping of the novel Phytophthora resistance gene in a Chinese soybean cultivar. Theor Appl Genet 126, 1555–1561 (2013). https://doi.org/10.1007/s00122-013-2073-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-013-2073-1

Keywords

Search

Navigation