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Molecular mapping and characterization of a single dominant gene controlling CMV resistance in peppers (Capsicum annuum L.)

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Abstract

Cucumber mosaic virus (CMV) is one of the most destructive viruses in the Solanaceae family. Simple inheritance of CMV resistance in peppers has not previously been documented; all previous studies have reported that resistance to this virus is mediated by several partially dominant and recessive genes. In this study, we showed that the Capsicum annuum cultivar ‘Bukang’ contains a single dominant resistance gene against CMVKorean and CMVFNY strains. We named this resistance gene Cmr1 (Cucumber mosaic resistance 1). Analysis of the cellular localization of CMV using a CMV green fluorescent protein construct showed that in ‘Bukang,’ systemic movement of the virus from the epidermal cell layer to mesophyll cells is inhibited. Genetic mapping and FISH analysis revealed that the Cmr1 gene is located at the centromeric region of LG2, a position syntenic to the ToMV resistance locus (Tm-1) in tomatoes. Three SNP markers were developed by comparative genetic mapping: one intron-based marker using a pepper homolog of Tm-1, and two SNP markers using tomato and pepper BAC sequences mapped near Cmr1. We expect that the SNP markers developed in this study will be useful for developing CMV-resistant cultivars and for fine mapping the Cmr1 gene.

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Acknowledgments

This work was supported by a grant (Code20070401034028) from the BioGreen21 program, Rural Development Administration, Republic of Korea and by a grant (GC1132-1) from the Crop Functional Genomics Center of the 21st Century Frontier Research Program, funded by the Ministry of Education, Science and Technology of the Republic of Korea.

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Authors and Affiliations

  1. Department of Plant Science, Research Institute for Agriculture and Life Sciences, Plant Genomics and Breeding Institute, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul, 151-921, Korea

    Won-Hee Kang, Ngoc Huy Hoang, Hee-Bum Yang, Jin-Kyung Kwon, Doil Choi & Byoung-Cheorl Kang

  2. Bioinformatics Research Center, KRIBB, Taejon, Korea

    Sung-Hwan Jo

  3. Department of Agricultural Biotechnology, Seoul National University, Seoul, 151-921, Korea

    Jang-Kyun Seo & Kook-Hyung Kim

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  1. Won-Hee Kang
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Correspondence to Byoung-Cheorl Kang.

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Communicated by I. Paran.

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Kang, WH., Hoang, N.H., Yang, HB. et al. Molecular mapping and characterization of a single dominant gene controlling CMV resistance in peppers (Capsicum annuum L.). Theor Appl Genet 120, 1587–1596 (2010). https://doi.org/10.1007/s00122-010-1278-9

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  • DOI: https://doi.org/10.1007/s00122-010-1278-9

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