Abstract
Phytophthora capsici causes devastating disease on many crop species, including Capsicum. Resistance in Capsicum annuum is genetically and physiologically complex. A panel of Capsicum germplasm that included genotypes from both C. annuum and C. chinense showing highly resistant, highly susceptible and intermediate or tolerant responses to the pathogen, respectively, was screened with a series of randomly amplified polymorphic sequence primers to determine which genomic regions contribute to the highest level of resistance. One primer, OpD04, amplified a single band only in those C. annuum and C. chinense genotypes showing the highest level of resistance. The amplified product was cloned, sequenced and used to design longer primers in order to generate a sequence characterized amplified region marker which was then mapped in a reference mapping population and a screened population segregating for resistance to P. capsici. These primers were observed to define a locus on pepper chromosome 5 tightly linked to Phyto.5.2, one of six quantitative trait loci (QTL) previously reported to contribute to P. capsici resistance. These results indicate that the Phyto.5.2 QTL may be widely distributed in highly resistant germplasm and provide improved resolution for this QTL. This work also defines the first breeding tools for this system, allowing for the rapid selection of genotypes likely to be highly resistant to P. capsici.
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Acknowledgements
This work was funded in part by USDA-IFAFS Plant Genome Award No. 2001-52100-113347 with additional support from NSF Plant Genome Award No. 0218166 and from the California Pepper Commission/California Pepper Improvement Foundation, the Vegetable Breeding Institute at Cornell University and Seminis. The QTL analysis was funded in part by NIH-MBRS-SCORE Award No. 5S06GM061223 to Dr. James Prince. We acknowledge important contributions of materials, protocols and critical review of the manuscript from Bob Heisey, and for DNA samples from Seminis, Mark Lewis, Sakata Seed America, Leon Mur and Nanne Faber, Enza Zaden and Steve Czaplewski, Syngenta Seeds. We thank George Moriarty for assistance in the greenhouse, Mary Kreitinger, Julie Izykowski and Liz Cirulli for technical assistance.
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Quirin, E.A., Ogundiwin, E.A., Prince, J.P. et al. Development of sequence characterized amplified region (SCAR) primers for the detection of Phyto.5.2, a major QTL for resistance to Phytophthora capsici Leon. in pepper. Theor Appl Genet 110, 605–612 (2005). https://doi.org/10.1007/s00122-004-1874-7
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DOI: https://doi.org/10.1007/s00122-004-1874-7