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Mapping of AFLP markers linked to seed coat colour loci in Brassica juncea (L.) Czern

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Abstract

Association mapping of the seed-coat colour with amplified fragment length polymorphism (AFLP) markers was carried out in 39 Brassica juncea lines. The lines had genetically diverse parentages and varied for seed-coat colour and other morphological characters. Eleven AFLP primer combinations were used to screen the 39 B. juncea lines, and a total of 335 polymorphic bands were detected. The bands were analysed for association with seed-coat colour using multiple regression analysis. This analysis revealed 15 markers associated with seed-coat colour, obtained with eight AFLP primer combinations. The marker E-ACA/M-CTG350 explained 69% of the variation in seed-coat colour. This marker along with markers E-AAC/M-CTC235 and E-AAC/M-CTA250 explained 89% of the total variation. The 15 associated markers were validated for linkage with the seed-coat colour loci using a recombinant inbred line (RIL) mapping population. Bands were amplified with the eight AFLP primer combinations in 54 RIL progenies. Of the 15 associated markers, 11 mapped on two linkage groups. Eight markers were placed on linkage group 1 at a marker density of 6.0 cM, while the remaining three were mapped on linkage group 2 at a marker density of 3.6 cM. Marker E-ACA/M-CTG350 co-segregated with Gene1 controlling seed-coat colour; it was specific for yellow seed-coat colour and mapped to linkage group 1. Marker E-AAC/M-CTC235 (AFLP8), which had been studied previously, was present on linkage group 2; it was specific for brown seed-coat colour. Since AFLP markers are not adapted for large-scale applications in plant breeding, it is important to convert these to sequence-characterised amplified region (SCAR) markers. Marker E-AAC/M-CTC235 (AFLP8) had been previously converted into a SCAR. Work is in progress to convert the second of the linked markers, E-ACA/M-CTG350, to a SCAR. The two linked AFLP markers converted to SCARs will be useful for developing yellow-seeded B. juncea lines by means of marker-assisted selection.

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Acknowledgements

The authors are grateful to Dr. R.K. Pachauri, Director General, TERI, and Dr. T.P. Singh, Director, TERI School of Advanced Studies, for their kind support and encouragement. The Senior Research Fellowship from the Council of Scientific and Industrial Research to Vaishali Sabharwal is duly acknowledged.

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Authors and Affiliations

  1. TERI School of Advanced Studies (TERI SAS), Lodhi Road, Darbari Seth Block, Habitat Place, 110 003, New Delhi, India

    V. Sabharwal

  2. Plant Molecular Biology Laboratory, Bioresources and Biotechnology Division, The Energy and Resources Institute (TERI), Lodhi Road, Darbari Seth Block, Habitat Place, 110 003, New Delhi, India

    M. Lakshmikumaran

  3. Department of Plant Breeding, Punjab Agricultural University, 141 004, Ludhiana, India

    S. S. Banga

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  1. V. Sabharwal
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  2. M. S. Negi
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  3. S. S. Banga
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  4. M. Lakshmikumaran
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Correspondence to M. Lakshmikumaran.

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Communicated by H.F. Linskens

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Sabharwal, V., Negi, M.S., Banga, S.S. et al. Mapping of AFLP markers linked to seed coat colour loci in Brassica juncea (L.) Czern. Theor Appl Genet 109, 160–166 (2004). https://doi.org/10.1007/s00122-004-1610-3

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  • DOI: https://doi.org/10.1007/s00122-004-1610-3

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