Abstract
The presence of a major resistance gene (Bru1) for brown rust in the sugarcane cultivar R570 (2n about 115) was confirmed by analyzing segregation of rust resistance in a large population of 658 individuals, derived from selfing of clone R570. A subset of this population was analyzed with AFLP and bulked segregant analysis (BSA) to develop a detailed genetic map around the resistance gene. Four hundred and forty three primer pairs were used resulting in the identification of eight AFLP markers surrounding the resistance gene in an interval of 10 cM, with the closest markers located at 1.9 and 2.2 cM on each side of the gene. Efficiency of the AFLP/BSA applied to the complex polyploid genome of sugarcane is discussed, as well as the potential of the newly identified AFLP markers for developing a map-based cloning approach exploiting, synteny conservation with sorghum.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Asnaghi C, Paulet F, Kaye C, Grivet L, Glaszmann JC, D’Hont A (2000) Application of synteny across the Poaceae to determine the map location of a rust resistance gene of sugarcane. Theor Appl Genet 10:962–969
Asnaghi C, D’Hont A, Glaszmann JC, Rott P (2001) Resistance of sugarcane cultivar R570 to Puccinia melanocephala from different geographic locations. Plant Dis 85:282–286
Bennetzen JL (2000) Comparative sequence analysis of plant nuclear genomes: microcolinearity and its many exceptions. Plant Cell 12:1021–1029
Bennetzen JL, Ramakrishna W (2002) Numerous small rearrangements of gene content, order and orientation differentiate grass genomes. Plant Mol Biol 48:821–7
Boivin K, Deu M, Rami J-F, Trouche, Hamon P (1999) Toward a saturated sorghum map using RFLP and AFLP markers. Theor Appl Genet 98:320–328
Bowers J, Schertz K, Abbey C, Anderson S, Chang C, Chittenden L, Draye X, Hoppe A, Jessup R, Lennington J, Lin Y, Liu S, Ming R, Qiang D, Reischmann K, Skinner N, Wang Y, Paterson A (2000) A high-density 2,399-locus map of Sorghum. The Poster in Plant Genome VIII
Chen M, SanMiguel P, de Oliveira AC, Woo SS, Zhang H, Wing RA, Bennetzen JL (1997) Microcolinearity in sh2-homologous regions of the maize, rice and sorghum genomes. Proc Natl Acad Sci USA 94:3431–3435
Comstock JC, Shine JM, Raid RN (1992a) Effect of early rust infection on subsequent sugar cane growth. Sugar Cane 4:7–9
Comstock JC, Wu KK, Schnell RJ (1992b) Heritability of resistance to sugar cane rust. Sugar Cane 6:7–10
D’Hont A, Lu YH, Gonzàlez de Leòn D, Grivet L, Feldmann P, Lanaud C, Glaszmann JC (1994) A molecular approach to unraveling the genetics of sugarcane, a complex polyploid of the Andropogoneae tribe. Genome 37:222–230
D’Hont A, Grivet L, Feldmann P, Rao P, Berding N, Glaszmann JC (1996) Characterisation of the double genome structure of modern sugarcane cultivars (Saccharum spp.) by molecular cytogenetics. Mol Gen Genet 250:405–413
Daugrois JH, Grivet L, Roques D, Hoarau JY, Lombard H, Glaszmann JC, D’Hont A (1996) A putative major gene for rust resistance linked with a RFLP marker in sugarcane cultivar “R570”. Theor Appl Genet 92:1059–1064
Dufour P, Grivet L, D’Hont A, Deu M, Trouche G, Glaszmann JC, Hamon P (1996) Comparative genetic mapping between duplicated segments on maize chromosomes 3 and 8 and homoeologous regions in sorghum and sugarcane. Theor Appl Genet 92:1024–1030
Dufour P, Deu M, Grivet L, D’Hont A, Paulet F, Bouet A, Lanaud C, Glaszmann JC, Hamon P (1997) Construction of a composite sorghum genome map and comparison with sugarcane, a related complex polyploid. Theor Appl Genet 94:409–418
Dunford RP, Kurata N, Laurie DA, Money TA, Minobe Y, Moore G (1995) Conservation of fine-scale DNA marker order in the genomes of rice and Triticeae. Nucleic Acids Res 23:2724–2728
Foote T, Roberts M, Kurata N, Sasaki T, Moore G (1997) Detailed comparative mapping of cereal chromosome regions corresponding to the Ph1 locus in wheat. Genetics 147:801–807
Gallego F, Feuillet C, Messner M, Penger A, Graner A, Yano M, Sasaki T, Keller B (1998) Comparative mapping of the two wheat leaf-rust resistance loci Lr1 and Lr10 in rice and barley. Genome 41:328–336
Glaszmann JC, Dufour P, Grivet L, D’Hont A, Deu M, Paulet F, Hamon P (1997) Comparative genome analysis between several tropical grasses. Euphytica 96:13–21
Grivet L, D’Hont A, Dufour P, Hamon P, Roques D, Glaszmann JC (1994) Comparative genome mapping of sugarcane with other species within the Andropogoneae tribe. Heredity 73:500–508
Grivet L, D’Hont A, Roques D, Feldmann P, Lanaud C, Glaszmann JC (1996) RFLP mapping in cultivated sugarcane (Saccharum spp.): genome organization in a highly polyploid and aneuploid interspecific hybrid. Genetics 142:987–1000
Guimaraes CT, Sills GR, Sobral BW (1997) Comparative mapping of Andropogoneae: Saccharum L (sugarcane) and its relation to sorghum and maize. Proc Natl Acad Sci USA 94:14261–14266
Hogarth DM, Ryan CC, Taylor PWJ (1993) Quantitative inheritance of rust resistance in sugarcane. Field Crops Res 34:187–193
Hoarau JY, Offmann B, D’Hont A, Risterucci AM, Roques D, Glaszmann JC, Grivet L (2001) Genetic dissection of a modern cultivar (Saccharum spp.). I. Genome mapping with AFLP. Theor Appl Genet 103:84–97
Hoisington D (1992) Laboratory protocols, CIMMYT. Applied Molecular Genetics Laboratory, Mexico, DF CIMMYT
Kilian A, Kudrna DA, Kleinhofs A, Yano M, Kurata N, Steffenson B, Sasaki T (1995) Rice-barley synteny and its application to saturation mapping of the barley Rpg1 region. Nucleic Acids Res 23:2729–2733
Lander E, Green P, Abrahamson J, Barlow A, Daly MJ Lincoln SE, Newberg S (1987) Mapmaker: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using a segregating population. Proc Natl Acad Sci USA 88:9828–9832
Ming R, Liu SC, Lin YR, Da Silva J, Wilson W, Braga D, Van Deynze A, Wenslaff TF, Wu KK, Moore PH, Burnquist W, Sorrels ME, Irvine JE, Paterson AH (1998) Detailed alignment of Saccharum and sorghum chromosomes: comparative organization of closely related diploid and polyploid genomes. Genetics 150:1663–1682
Ramdoyal R, Sullivan S, Lim Shin Chong L, Badaloo G, Sautally S, Domaingue R (2000) The genetics of rust resistance in sugarcane seedling populations. Theor Appl Genet 100:557–563
Tai PYP, Miller JD, Dean JL (1981) Inheritance of resistance to rust in the sugarcane field. Crops Res 4:261–268
Tomkins JP, Yu Y, Miller-Smith H, Frisch DA, Woo SS, Wing R (1999) A bacterial artificial chromosome library for sugarcane. Theor Appl Genet 99:419–424
Vos P, Hogers R, Bleeker M, Reijans M, van der Lee T, Hornes M, Fritjers A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414
Wu KK, Burnquist W, Sorrells ME, Tew TL, Moore PH, Tanksley SD (1992) The detection and estimation of linkage in polyploids using single-dose restriction fragments. Theor Appl Genet 83:294–300
Acknowledgments
We thank the International Consortium for Sugarcane Biotechnology for the financial support of this work, and J.C. Glaszmann for critical reading of the manuscript.
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by H. F. Linskens
Rights and permissions
About this article
Cite this article
Asnaghi, C., Roques, D., Ruffel, S. et al. Targeted mapping of a sugarcane rust resistance gene (Bru1) using bulked segregant analysis and AFLP markers. Theor Appl Genet 108, 759–764 (2004). https://doi.org/10.1007/s00122-003-1487-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00122-003-1487-6