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The anti-inflammatory effects of platinum nanoparticles on the lipopolysaccharide-induced inflammatory response in RAW 264.7 macrophages

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Abstract

Objective

Platinum nanoparticles (nano-Pt) have been reported to possess anti-oxidant and anti-tumor activities. However, the biological activity and mechanism of action of nano-Pt in inflammation are still unknown. The present study was designed to determine the in-vitro anti-inflammatory effects of nano-Pt on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.

Methods

RAW 264.7 macrophages were used for the study. The LPS-induced production of reactive oxygen species (ROS) was determined by flow cytometry. The prostaglandin E2 (PGE2) concentration was measured using a PGE2 assay kit. The protein levels and mRNA expression of the pro-inflammatory cytokines [tumor necrosis factor-α, interleukin (IL)-1β and IL-6], along with cyclooxygenase (COX-2) and inducible nitric oxide synthase, were analyzed by Western blotting and reverse transcription–polymerase chain reaction analysis. The phosphorylation of extracellular signal regulated kinase (ERK1/2) and Akt, and the phosphorylation and degradation of inhibitory kappa B-alpha (IκB-α) was determined by Western blot analysis.

Results

Nano-Pt significantly reduced the LPS-induced production of intracellular ROS and inflammatory mediators. In addition, nano-Pt suppressed the phosphorylation of ERK1/2 and Akt, and significantly inhibited the phosphorylation/degradation of IκB-α as well as nuclear factor kappa-B (NFκB) transcriptional activity.

Conclusion

These results suggest that the anti-inflammatory properties of nano-Pt may be attributed to their downregulation of the NFκB signaling pathway in macrophages, thus supporting the use of nano-Pt as an anti-inflammatory agent.

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Abbreviations

Nano-Pt:

Platinum nanoparticles

RT:

Reverse transcription

PGE2 :

Prostaglandin E2

iNOS:

Inducible nitric oxide synthase

MAPK:

Mitogen activated protein kinases

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Acknowledgments

This research was supported by a Grant-in-Aid for Scientific Research (No. 20591337) from the Japan Society for the Promotion of Science.

Conflict of interest

The authors declare no conflicts of interest.

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Authors and Affiliations

  1. Department of Dermatology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Sugitani, Toyama, Japan

    Mati Ur Rehman, Yoko Yoshihisa & Tadamichi Shimizu

  2. Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Chiba, Japan

    Yusei Miyamoto

Authors
  1. Mati Ur Rehman
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  2. Yoko Yoshihisa
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  3. Yusei Miyamoto
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  4. Tadamichi Shimizu
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Corresponding author

Correspondence to Tadamichi Shimizu.

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Responsible Editor: Graham Wallace.

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Rehman, M.U., Yoshihisa, Y., Miyamoto, Y. et al. The anti-inflammatory effects of platinum nanoparticles on the lipopolysaccharide-induced inflammatory response in RAW 264.7 macrophages. Inflamm. Res. 61, 1177–1185 (2012). https://doi.org/10.1007/s00011-012-0512-0

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  • DOI: https://doi.org/10.1007/s00011-012-0512-0

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