Abstract
Objective:To investigate whether ketamine could inhibit lipopolysaccharide (LPS)-induced intracellular calcium elevation and NF-kappa B activation in monocytes.
Materials and methods:Isolated rat monocytes were challenged with 10 μg/ml LPS with or without the presence of various concentrations of ketamine (10, 100, 1000 μM). Intracellular calcium was monitored by laser confocal microscopy. NF-kappa B activity of the nuclear extracts of monocytes was analyzed by electrophoretic mobility shift assay (EMSA).
Results:LPS provoked a significant calcium elevation and enhanced NF-kappa B activity in monocytes. Ketamine above concentration of 100 μM inhibited endotoxin-induced intracellular calcium elevation and NF-kappa B activity. Ketamine itself had no effect on either of them.
Conclusions:These findings suggest that ketamine could suppress NF-kappa B in monocytes exposed to endotoxin, and this anti-inflammatory effect might act through attenuating intracellular calcium elevation.
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Received 31 October 2003; returned for revision 18 December 2003; accepted by I. Ahnfelt-Rønne 26 Januaryy 2004
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Sun, J., Zhou, Z.Q., Lv, R. et al. Ketamine inhibits LPS-induced calcium elevation and NF-kappa B activation in monocytes. Inflamm. res. 53, 304–308 (2004). https://doi.org/10.1007/s00011-004-1262-4
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DOI: https://doi.org/10.1007/s00011-004-1262-4