Summary
Fusion of unilamellar lipid vesicles with red blood cells has been used to deliver entrapped inositol hexaphosphate (IHP) to the cells. Incorporated IHP tightly bound to hemoglobin produces a significant and lasting “right-shift” of the O2-binding curve of red blood cells. The O2 release capacity of red blood cells is increased by this method up to 270 % of the normal value. The Bohr effect is increased so that carbon dioxide transport is also enhanced. The fine structure of the erythrocytes does not seem to be altered by the lipid vesicle incorporation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Benesch, R., Benesch, R.E.: The effect of organic phosphates from the human erythrocyte on the allosteric properties of hemoglobin. Biophys. Biochem. Res. Commun.26, 162–167 (1967)
Chanutin, A., Curnish, R.R.: Effect of organic and inorganic phosphates on the oxygen equilibrum of human erythrocytes. Arch. Biochem. Biophys.121, 96–105 (1967)
Rapoport, S., Luebering, J.: The formation of 2,3-diphosphoglycerate in rabbit erythrocytes: The existence of a diphosphoglycerate mutase. J. Biol. Chem.183, 507–516 (1950)
Imai, K., Yonetani, T.: Thermodynamical studies of oxygen equilibrium of hemoglobin. Nonuniform heats and entropy changes for the individual oxygenation steps and enthalpyentropy compensation. J. Biol. Chem.250, 7093–7098 (1975)
Ruckpaul, K., Rein, H., Ristau, O., Jänig, G.-R., Jung, F.: Interaction of hemoglobin with ions. Allosteric effects of the binding of anions. Biochim. Biophys. Acta236, 211–221 (1971)
Nicolau, C., Gersonde, K.: Incorporation of inositol hexaphosphate into intact red blood cells. I. Fusion of effector-containing lipid vesicles with erythrocytes. Naturwissenschaften (submitted) (1979)
Gersonde, K., Nicolau, C.: Incorporation of inositol hexaphosphate into intact red blood cells. II. Enhacement of the gas transport. Naturwissenschaften (submitted) (1979)
Poste, G., Papahadjopoulos, D., Vail, W.J.: Lipid vesicles as carrier for introducing biologically active materials into cells. Methods in cell biology, Vol. XIV. Prescott, D.M., (ed.), pp. 33–71. New York: Academic Press 1976
Pagano, R.E., Weinstein, J.N.: Interactions of liposomes with mammalian cells. Ann. Rev. Biophys. Bioeng.7, 435–468 (1978)
Blumenthal, R., Weinstein, J.N., Sharrow, S.O., Henkart, P.: Liposomes-lymphocyte interaction: Saturable sites for transfer and intracellular release of liposome contents. Proc. Natl. Acad. Sci. USA74, 5603–5697 (1977)
Sick, H., Gersonde, K.: Method for continuous registration of O2-binding curves of hemoproteins by means of a diffusion chamber. Anal. Biochem.32, 362–376 (1969)
Sick, H., Gersonde, K.: Theory and application of the diffusion technique for measurement and analysis of O2-binding properties of very autoxidizable hemoproteins. Anal. Biochem.47, 46–56 (1972)
Benesch, R.E., Edalji, R., Benesch, R.: Reciprocal interaction of hemoglobin with oxygen and protons. The influence of allosteric polyanions. Biochemistry16, 2594–2597 (1977)
Rossi-Bernardi, L., Roughton, F. J. W.: The specific influence of carbon dioxide and carbamate compounds on the buffer power and Bohr effects in human haemoglobin solutions. J. Physiol. (London)189, 1–20 (1967)
Johnson, S.M., Nicolau, C.: The distribution of 1,6-diphenyl hexatrien fluorescence in normal human lymphocytes. Biophys. Biochem. Res. Commun.76, 869–874 (1977)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gersonde, K., Nicolau, C. Improvement of the red blood cell O2 release capacity by lipid vesicle-mediated incorporation of inositol hexaphosphate. Blut 39, 1–7 (1979). https://doi.org/10.1007/BF01008069
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01008069