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Foliar application of Bacillus subtilis AUBS1 reduces sheath blight and triggers defense mechanisms in rice

Blattapplikation von Bacillus subtilis AUBS1 reduziert die Blattscheidendürre und induziert Abwehrmechanismen in Reis

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Abstract

Foliar application of Bacillus subtilis strain AUBS1 significantly reduced the sheath blight disease of rice (Oryza sativa L.) under greenhouse conditions. Treatment with B. subtilis led to an increase in the activities of phenylalanine ammonia-lyase (PAL) and peroxidase (PO) and an accumulation of pathogenesis-related (PR) proteins in rice leaves. Application of B. subtilis also induced an accumulation of a thaumatin-like protein (17 kDa) and two ß-1-3-glucanases (30 kDa and 33 kDa). Western blotting analysis for chitinase revealed the induction of two chitinases with apparent molecular masses of 30 kDa and 35 kDa in the induced plants. The coordinate upregulation of different defense mechanisms in induced plants suggests that these defense responses may be involved in the restriction of Rhizoctonia solani.

Zusammenfassung

Blattapplikation von Bacillus subtilis Stamm AUBSl reduzierte unter Gewächshausbedingungen die durch Rhizoctonia solani verursachte Blattscheidendürre an Reis (Oryza sativa L.). Behandlung mit B. subtilis führte zu einer Zunahme der Aktivitäten von Phenylalaninammonium-Lyase (PAL) und Peroxidase (PO) sowie zu einer Akkumulation von Pr-Proteinen in Reisblättern. Ferner wurde die Zunahme eines Thaumatin ähnlichen Proteins (17 kDa) und von zwei ß-1,3-Glucanasen (30 kDa und 33 kDa) induziert. Durch die western blotting-Analyse für Chitinasen konnte die Induktion von zwei Chitinasen mit den molekularen Massen von 30 kDa und 35 kDa nachgewiesen werden. Die koordinierte Regulierung von verschiedenen Abwehrmechanismen in induzierten Pflanzen deutet darauf hin, dass diese Abwehrreaktionen an der Restriktion von Rhizoctonia solani beteiligt sind.

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Authors and Affiliations

  1. Department of Biochemistry, Kansas State University, Manhattan, Kansas, 66506, USA

    J. Jayaraj & S. Muthukrishnan

  2. Department of Agronomy, Kansas State University, Manhattan, Kansas, 66506, USA

    H. Yi & G. H. Liang

  3. Department of Plant Pathology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai, 625 104, Tamil Nadu, India

    R. Velazhahan

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  1. J. Jayaraj
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Jayaraj, J., Yi, H., Liang, G.H. et al. Foliar application of Bacillus subtilis AUBS1 reduces sheath blight and triggers defense mechanisms in rice. J Plant Dis Prot 111, 115–125 (2004). https://doi.org/10.1007/BF03356138

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