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Correlation between porcinePPARGC1A mRNA expression and its downstream target genes in backfat andlongissimus dorsi muscle

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Abstract

Knowledge of in vivo relationship between the coactivatorPPARGC1A and its target genes is very limited, especially in the pig. In this study, a real-time PCR experiment was performed onlongissimus dorsi muscle (MLD) and backfat with 10 presumedPPARGC1A downstream target genes, involved in energy and fat metabolism, to identify possible relationships withPPARGC1A mRNA expression in vivo in the pig (n = 20). Except forUCP3 andLPL, a very significant difference in expression was found between MLD and backfat for all genes (P < 0.01). Hierarchical cluster analysis and the significant pairing of mRNA expression data between sampling locations suggested a genetic regulation of the expression of several target genes. A positive correlation withPPARGC1A was found forCPT1B, GLUT4, PDK4, andTFAM (P < 0.0001). A negative correlation was found forUCP2, FABP4, LEP (P < 0.0001), andTNF (P = 0.0071). No significant correlation was detected forUCP3 andLPL. This study provides evidence for a clear difference in mRNA expression of crucial genes in fat and energy metabolism between 2 important tissues. Our data suggest a clear impact ofPPARGC1A on energy and lipid metabolism in vivo in the pig, through several of these downstream target genes.

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Authors and Affiliations

  1. Department of Nutrition, Genetics and Ethology, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, 9820, Merelbeke, Belgium

    T. Erkens, A. Van Zeveren & L. J. Peelman

  2. Centre for Medical Genetics Ghent, Ghent University Hospital, Belgium

    J. Vandesompele

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  1. T. Erkens
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  2. J. Vandesompele
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  3. A. Van Zeveren
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  4. L. J. Peelman
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Correspondence to T. Erkens.

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Erkens, T., Vandesompele, J., Van Zeveren, A. et al. Correlation between porcinePPARGC1A mRNA expression and its downstream target genes in backfat andlongissimus dorsi muscle. J Appl Genet 50, 361–369 (2009). https://doi.org/10.1007/BF03195694

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  • DOI: https://doi.org/10.1007/BF03195694

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