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Aerobic reduction of perchlorate by bacteria isolated in Kerala, South India

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Abstract

Possibility of perchlorate reduction by microbes raises hope for an eco-friendly mode of degradation of this toxic rocket fuel. This study reports 3 isolates (A1, A2 and A3) capable of molybdenum-independent degradation of perchlorate under aerobic conditions. The rate of degradation was the highest when perchlorate concentration was 17 mM, and then 3.2 mM, 4.7 mM and 4.1 mM of perchlorate was reduced by isolates A1, A2 and A3 (respectively) after 72 h at 28°C under aerobic conditions. Presence of perchlorate at a concentration higher than 17 mM resulted in some inhibition of perchlorate reduction. 16S ribosomal RNA gene analysis revealed isolate A1 to bePseudomonas stutzeri (Proteobacteria) while isolates A2 ad A3 where found to belong to the genusArthrobacter (Actinobacteria). The study, apart from demonstrating ribotyping as a rapid method of identification of economically important soil microbes, also raised prospects for using artificial consortia for environmental degradation of perchlorate, without apparent domination ofDechloromonas spp. (a group of microbes known for perchlorate remediation in the environment).

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Authors and Affiliations

  1. Genomics Division, geneOmbio Technologies, Krishna Chambers, Pashan, Maharashtra, India

    Anita Shete, Pratap N. Mukhopadhyaya, Arpan Acharya & Bikash A. Aich

  2. Department of Chemistry, University of Pune, Maharashtra, India

    Suresh Joshi & Vikram S. Ghole

  3. R&D Operations, geneOmbio Technologies, Krishna Chambers, 4th Floor, Pashan-Sus Road, Pashan, Pune, 411 021, Maharashtra, India

    Pratap N. Mukhopadhyaya

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  1. Anita Shete
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  2. Pratap N. Mukhopadhyaya
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  3. Arpan Acharya
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  4. Bikash A. Aich
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  5. Suresh Joshi
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  6. Vikram S. Ghole
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Correspondence to Pratap N. Mukhopadhyaya.

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Shete, A., Mukhopadhyaya, P.N., Acharya, A. et al. Aerobic reduction of perchlorate by bacteria isolated in Kerala, South India. J Appl Genet 49, 425–431 (2008). https://doi.org/10.1007/BF03195643

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